1KN6

Solution Structure of the Mouse Prohormone Convertase 1 Pro-Domain

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D NOESY	100mM phosphate buffer; uniform (random) labeling with 15N, 13C	90% H2O/10% D2O	2mM	7.0	ambient	298
2	3D_13C-separated_NOESY	100mM phosphate buffer; uniform (random) labeling with 15N, 13C	90% H2O/10% D2O	2mM	7.0	ambient	298
3	3D_15N-separated_NOESY	100mM phosphate buffer; uniform (random) labeling with 15N, 13C	90% H2O/10% D2O	2mM	7.0	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DRX	600
2	Bruker	DRX	500

NMR Refinement
Method	Details	Software
simulated annealing		NMRPipe

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	280
Conformers Submitted Total Number	20
Representative Model	3 (closest to the average)

Additional NMR Experimental Information
Details	This structure was determined using standard 3D heteronuclear techniques

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	processing	NMRPipe	1.8	Delaglio, F., et al

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.