1K3K

Solution Structure of a Bcl-2 Homolog from Kaposi's Sarcoma Virus

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	HNCA	0.5-1.0 MM PROTEIN, 20 mM2 H-TRIS, 5 mM2 H-dithiothreitol		20 mM	7.8	ATMOSPHERIC atm	298
2	HN(CO)CA	0.5-1.0 MM PROTEIN, 20 mM2 H-TRIS, 5 mM2 H-dithiothreitol		20 mM	7.8	ATMOSPHERIC atm	298
3	HN(CA)CB	0.5-1.0 MM PROTEIN, 20 mM2 H-TRIS, 5 mM2 H-dithiothreitol		20 mM	7.8	ATMOSPHERIC atm	298
4	HN(COCA)CB	0.5-1.0 MM PROTEIN, 20 mM2 H-TRIS, 5 mM2 H-dithiothreitol		20 mM	7.8	ATMOSPHERIC atm	298
5	HNCO	0.5-1.0 MM PROTEIN, 20 mM2 H-TRIS, 5 mM2 H-dithiothreitol		20 mM	7.8	ATMOSPHERIC atm	298
6	HN(CA)CO	0.5-1.0 MM PROTEIN, 20 mM2 H-TRIS, 5 mM2 H-dithiothreitol		20 mM	7.8	ATMOSPHERIC atm	298
7	13C-NOESY	0.5-1.0 MM PROTEIN, 20 mM2 H-TRIS, 5 mM2 H-dithiothreitol		20 mM	7.8	ATMOSPHERIC atm	298
8	15N-NOESY	0.5-1.0 MM PROTEIN, 20 mM2 H-TRIS, 5 mM2 H-dithiothreitol		20 mM	7.8	ATMOSPHERIC atm	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DRX	500
2	Bruker	DRX	600
3	Bruker	DRX	800

NMR Refinement
Method	Details	Software
simulated annealing		CNX

NMR Ensemble Information
Conformer Selection Criteria	AVERAGE, MINIMIZED
Conformers Calculated Total Number	100
Conformers Submitted Total Number	1

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	structure solution	CNX
2	refinement	CNX

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.