1K2G

Structural basis for the 3'-terminal guanosine recognition by the group I intron

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D NOESY	10mM Phosphate buffer NA	99% D2O	100mM	5.5	ambient	283
2	DQF-COSY	10mM Phosphate buffer NA	99% D2O	100mM	5.5	ambient	283
3	2D NOESY	10mM Phosphate buffer NA	99% D2O	100mM	5.5	ambient	278

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DRX	600
2	Bruker	DMX	750

NMR Refinement
Method	Details	Software
simulated annealing molecular dynamics		X-PLOR

NMR Ensemble Information
Conformer Selection Criteria
Conformers Calculated Total Number
Conformers Submitted Total Number	1
Representative Model	(lowest energy)

Additional NMR Experimental Information
Details	This structure was determined using standard 2D homonuclear techniques

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	structure solution	X-PLOR	3.8	Brunger
2	data analysis	Felix	950
3	refinement	X-PLOR	3.8	Brunger

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.