1JJR
The Three-Dimensional Structure of the C-terminal DNA Binding Domain of Human Ku70
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_13C-separated_NOESY | 1.0 mM Ku70_CTD 15N,13C; 100mM Phosphate buffer pH6.0 | 100% D2O | 100 mM Na phosphate | 6 | 1 atm | 293 | |
| 2 | 3D_15N-separated_NOESY | 0.8 mM Ku70_CTD 15N; 100mM Phosphate buffer pH6.0 | 90% H2O/10% D2O | 100 mM Na phosphate | 6 | 1 atm | 293 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | UNITYPLUS | 500 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | Felix | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (lowest energy) |
| Additional NMR Experimental Information | |
|---|---|
| Details | The structure was determined using triple-resonance NMR spectroscopy. |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | processing | Felix | 98 | MSI |
| 2 | collection | VNMR | 6.1B | Varian |
| 3 | structure solution | DYANA | 1.5 | Peter Gntert, Christian Mumenthaler & Torsten Herrmann |
| 4 | structure solution | X-PLOR | CNS 1.0 | A.T.Brunger, P.D.Adams, G.M.Clore, W.L.Delano, P.Gros, R.W.Grosse-Kunstleve, |
| 5 | refinement | X-PLOR | CNS 1.0 | A.T.Brunger, P.D.Adams, G.M.Clore, W.L.Delano, P.Gros, R.W.Grosse-Kunstleve, |
