1IUR
DnaJ domain of human KIAA0730 protein
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_13C-separated_NOESY | 1.3MM PROTEIN, 20MM SODIUM PHOSPHATE, 100MM SODIUM CHLORIDE, 90% H2O, 10% D2O | 20mM Sodium Phosphate, 100mM Sodium chloride, 0.02% Sodium azide | 100mM | 6.0 | ambient | 298 | |
| 2 | 3D_15N-separated_NOESY | 1.3MM PROTEIN, 20MM SODIUM PHOSPHATE, 100MM SODIUM CHLORIDE, 90% H2O, 10% D2O | 20mM Sodium Phosphate, 100mM Sodium chloride, 0.02% Sodium azide | 100mM | 6.0 | ambient | 298 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AVANCE | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | XwinNMR | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 50 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (lowest energy) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | collection | XwinNMR | 2.6 | |
| 2 | processing | NMRPipe | 1.8 | Delaglio |
| 3 | data analysis | NMRView | 5.03 | Johnson |
| 4 | structure solution | DYANA | Guentert | |
| 5 | refinement | CNS | 1.1 | Brunger |
