1IRZ

Solution structure of ARR10-B belonging to the GARP family of plant Myb-related DNA binding motifs of the Arabidopsis response regulators

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_15N-separated_NOESY	1mM ARR10-B[U-15N,13C]; 50mM sodium phosphate buffer 500mM NaCl	90% H2O/10% D2O	500mM NaCl	6.9	ambient	288
2	4D_13C-separated_NOESY	1mM ARR10-B[U-15N,13C]; 50mM sodium phosphate buffer 500mM NaCl	100% D2O	500mM NaCl	6.9	ambient	288
3	HCCH-TOCSY	1mM ARR10-B[U-15N,13C]; 50mM sodium phosphate buffer 500mM NaCl	100% D2O	500mM NaCl	6.9	ambient	288
4	CBCA(CO)NH	1mM ARR10-B[U-15N,13C]; 50mM sodium phosphate buffer 500mM NaCl	90% H2O/10% D2O	500mM NaCl	6.9	ambient	288
5	HNCO	1mM ARR10-B[U-15N,13C]; 50mM sodium phosphate buffer 500mM NaCl	90% H2O/10% D2O	500mM NaCl	6.9	ambient	288
6	HNCA	1mM ARR10-B[U-15N,13C]; 50mM sodium phosphate buffer 500mM NaCl	90% H2O/10% D2O	500mM NaCl	6.9	ambient	288

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DMX	750

NMR Refinement
Method	Details	Software
distance geometry simulated annealing		NMRPipe

NMR Ensemble Information
Conformer Selection Criteria	structures with lowest energies
Conformers Calculated Total Number	50
Conformers Submitted Total Number	15

Additional NMR Experimental Information
Details	The structure was determined by multidimensional heteronuclear NMR spectroscopy.

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	processing	NMRPipe	1.8	Frank Delaglio
2	refinement	X-PLOR	3.1	Brunger
3	data analysis	PIPP/CAPP/STAPP	4.3.2	Dan Garrett

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.