1I9F

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D NOESY	1.6 mM RNA/peptide	90% H2O/10% D2O, 20 mM sodium phosphate, 100 mM sodium chloride, 0.1 mM EDTA, pH 6.5	20mM sodium phosphate, 100 mM sodium chloride	6.5	1 atm	298
2	3D_13C-separated_NOESY	1.6 mM RNA/peptide	90% H2O/10% D2O, 20 mM sodium phosphate, 100 mM sodium chloride, 0.1 mM EDTA, pH 6.5	20mM sodium phosphate, 100 mM sodium chloride	6.5	1 atm	298
3	3D_15N-separated_NOESY	1.6 mM RNA/peptide	90% H2O/10% D2O, 20 mM sodium phosphate, 100 mM sodium chloride, 0.1 mM EDTA, pH 6.5	20mM sodium phosphate, 100 mM sodium chloride	6.5	1 atm	298
4	DQF-COSY	1.6 mM RNA/peptide	90% H2O/10% D2O, 20 mM sodium phosphate, 100 mM sodium chloride, 0.1 mM EDTA, pH 6.5	20mM sodium phosphate, 100 mM sodium chloride	6.5	1 atm	298
5	HNCA-J	1.6 mM RNA/peptide	90% H2O/10% D2O, 20 mM sodium phosphate, 100 mM sodium chloride, 0.1 mM EDTA, pH 6.5	20mM sodium phosphate, 100 mM sodium chloride	6.5	1 atm	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1			500
2			600

NMR Ensemble Information
Conformer Selection Criteria
Conformers Calculated Total Number	20
Conformers Submitted Total Number	7
Representative Model	(fewest violations, lowest energy)