1HO7
NMR STRUCTURE OF THE POTASSIUM CHANNEL FRAGMENT L45 IN TFE
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | DQF-COSY | 2mM NA-L45 | TFE:H2O ratio of 80:20 with 9% D2O | 0 | 2.7 | ambient | 300 | |
| 2 | TOCSY | 2mM NA-L45 | TFE:H2O ratio of 80:20 with 9% D2O | 0 | 2.7 | ambient | 300 | |
| 3 | NOESY | 2mM NA-L45 | TFE:H2O ratio of 80:20 with 9% D2O | 0 | 2.7 | ambient | 300 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA | 600 |
| 2 | Varian | INOVA | 750 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| Hybrid distance geometry/simulated annealing | DYANA | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | closest to average structure |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 1 |
| Representative Model | 1 (closest to the average) |
| Additional NMR Experimental Information | |
|---|---|
| Details | This structure was determined using standard 2D homonuclear techniques. |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | structure solution | DYANA | 1.5 | Guentert et al. |
| 2 | refinement | DYANA | 1.5 | Guentert et al. |
