1HHW
Solution structure of LNA1:RNA hybrid
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | NOESY | 0.1 | 7.0 | 1 atm | 298 | |||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA | 750 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | A TOTAL OF 284 NOE DISTANCE RESTRAINTS WERE OBTAINED FROM A FULL RELAXATION MATRIX ANALYSIS WITH THE PROGRAM RANDMARDI. 16 J-COUPLING CONSTANT RESTRAINTS WERE DERIVED FROM A J-SCALED-1H-31P-HMBC SPECTRUM. 22 WATSON-CRICK BASE PAIRING RESTRAINTS. | AMBER6 |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | |
| Conformers Calculated Total Number | 40 |
| Conformers Submitted Total Number | 40 |
| Representative Model | 7 (n/a) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | AMBER6 | CASE, DA, KOLLMAN, PA | |
| 2 | structure solution | MARDIGRAS | ||
| 3 | structure solution | AMBER | ||
| 4 | structure solution | FELIX | ||
