Experiment: 1EZO

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	4D_13C/15N-separated_NOESY	1.4 mM maltodextrin binding protein U-15N,13C,2H, with 1H amide and methyl on Val, Leu, and Ile(delta1) 2mM beta-cyclodextrin, 20 mM sodium phosphate pH 7.2, 3uM NaN3, 100uM EDTA.	90% H2O/10% D2O		7.2	ambient	310
2	4D_15N-separated_NOESY	1.4 mM maltodextrin binding protein U-15N,13C,2H, with 1H amide and methyl on Val, Leu, and Ile(delta1) 2mM beta-cyclodextrin, 20 mM sodium phosphate pH 7.2, 3uM NaN3, 100uM EDTA.	90% H2O/10% D2O		7.2	ambient	310
3	3D_13C-separated_NOESY	1.4 mM maltodextrin binding protein U-15N,13C,2H, with 1H amide and methyl on Val, Leu, and Ile(delta1) 2mM beta-cyclodextrin, 20 mM sodium phosphate pH 7.2, 3uM NaN3, 100uM EDTA.	90% H2O/10% D2O		7.2	ambient	310
4	(HM)CMCB(CMHM)-NOESY	1.4 mM maltodextrin binding protein U-15N,13C,2H, with 1H amide and methyl on Val, Leu, and Ile(delta1) 2mM beta-cyclodextrin, 20 mM sodium phosphate pH 7.2, 3uM NaN3, 100uM EDTA.	90% H2O/10% D2O		7.2	ambient	310

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	600

NMR Refinement
Method	Details	Software
simulated annealing from extended coordinates, torsion angle dynamics, and finish with cartesian dynamics		NMRPipe

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	384
Conformers Submitted Total Number	10

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	processing	NMRPipe	1.7
2	data analysis	NMRView	3.0
3	structure solution	CNS	0.5
4	refinement	CNS	0.5

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.