1ESK

SOLUTION STRUCTURE OF NCP7 FROM HIV-1


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
12D NOESY2mM (12-53)NCp7, 90%H2O, 10% D2O, pH 6.090% H2O/10% D2On.a.6.0ambient293
2DQF-COSY2mM (12-53)NCp7, 90%H2O, 10% D2O, pH 6.090% H2O/10% D2On.a.6.0ambient293
3TOCSY2mM (12-53)NCp7, 90%H2O, 10% D2O, pH 6.090% H2O/10% D2On.a.6.0ambient293
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerAMX600
NMR Refinement
MethodDetailsSoftware
simulated annealingthe structures are based on a total of 444 NOE-derived distance constraintsUXNMR
NMR Ensemble Information
Conformer Selection Criteriastructures with acceptable covalent geometry,structures with favorable non-bond energy,structures with the least restraint violations,structures with the lowest energy
Conformers Calculated Total Number50
Conformers Submitted Total Number9
Representative Model1 (lowest energy)
Additional NMR Experimental Information
DetailsThis structure was determined using standard 2D homonuclear techniques.
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionUXNMR940501Bruker
2refinementDiscoverMSI