1E95

Solution structure of the pseudoknot of SRV-1 RNA, involved in ribosomal frameshifting

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	NOESY			100 MM NACL, 10 MM K2HPO4/KH2PO4	6.4		303
2	DQF-COSY			100 MM NACL, 10 MM K2HPO4/KH2PO4	6.4		303
3	TOCSY			100 MM NACL, 10 MM K2HPO4/KH2PO4	6.4		303
4	31P-1H HETCOR			100 MM NACL, 10 MM K2HPO4/KH2PO4	6.4		303
5	HMQC			100 MM NACL, 10 MM K2HPO4/KH2PO4	6.4		303
6	31P-1H HETERO-TOCSY-NOESY			100 MM NACL, 10 MM K2HPO4/KH2PO4	6.4		303
7	NOESY-HMQC			100 MM NACL, 10 MM K2HPO4/KH2PO4	6.4		303
8	HCCH-TOCSY			100 MM NACL, 10 MM K2HPO4/KH2PO4	6.4		303

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DMX	500
2	Varian	INOVA	600
3	Varian	INOVA	750

NMR Refinement
Method	Details	Software
torsion angle dynamics		X-PLOR

NMR Ensemble Information
Conformer Selection Criteria	LEAST RESTRAINT VIOLATIONS, LOWEST ENERGY
Conformers Calculated Total Number	100
Conformers Submitted Total Number	15

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	refinement	X-PLOR	3.851	BRUNGER
2	structure solution	XPLOR

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.