1BYN
SOLUTION STRUCTURE OF THE CALCIUM-BOUND FIRST C2-DOMAIN OF SYNAPTOTAGMIN I
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D | 100 mM NACL | 5.0 | 307 | ||||
| 2 | 3D AND 4D HOMONUCLEAR | 100 mM NACL | 5.0 | 307 | ||||
| 3 | HETERONUCLEAR | 100 mM NACL | 5.0 | 307 | ||||
| 4 | TRIPLE RESONANCE EXPERIMENTS | 100 mM NACL | 5.0 | 307 | ||||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | UNITY 500 | 500 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | Discover | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | FEWEST RESTRAINTS VIOLATIONS |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 20 |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | Discover | BIOSYM | |
| 2 | structure solution | Discover | ||
| 3 | structure solution | NMRCHITECT | ||
