1BVJ

HIV-1 RNA A-RICH HAIRPIN LOOP

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	NOESY			10mM NA PHOSPHATE	6.4		283
2	COSY 2D			10mM NA PHOSPHATE	6.4		283
3	COSY 3D			10mM NA PHOSPHATE	6.4		283

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	500

NMR Refinement
Method	Details	Software
simulated annealing	SEE REFERENCE ABOVE	NMRCHITECT

NMR Ensemble Information
Conformer Selection Criteria	LOWEST TOTAL AND RESTRAINT VIOLATION ENERGIES
Conformers Calculated Total Number	160
Conformers Submitted Total Number	21
Representative Model	14 (n/a)

Additional NMR Experimental Information
Details	THE STRUCTURE WAS DETERMINED USING TRIPLE RESONANCE HCN AND HCP EXPERIMENTS ON 15N,13C-LABELED RNA.

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	refinement	NMRCHITECT		MSI
2	structure solution	MSI NMRCHITECT	NMRCHITECT

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.