1BJ2
RNA LOOP-LOOP COMPLEX: THE COLE1 INVERTED LOOP SEQUENCE, NMR, 8 STRUCTURES
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | NOESY | H2O | 25 mM | 6.5 | 1 atm | 308 | ||
| 2 | DQF-COSY | H2O | 25 mM | 6.5 | 1 atm | 308 | ||
| 3 | 15N/14N SELECTED NOESY | H2O | 25 mM | 6.5 | 1 atm | 308 | ||
| 4 | 13C/12C SELECTED NOESY | H2O | 25 mM | 6.5 | 1 atm | 308 | ||
| 5 | HCCH-COSY | H2O | 25 mM | 6.5 | 1 atm | 308 | ||
| 6 | HCCH-COSY | H2O | 25 mM | 6.5 | 1 atm | 308 | ||
| 7 | 31P | H2O | 25 mM | 6.5 | 1 atm | 308 | ||
| 8 | MQHCN | H2O | 25 mM | 6.5 | 1 atm | 308 | ||
| 9 | 13C-HMQC | H2O | 25 mM | 6.5 | 1 atm | 308 | ||
| 10 | 15N-HMQC | H2O | 25 mM | 6.5 | 1 atm | 308 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | UNITY | 500 |
| 2 | GE | UNITY | 600 |
| 3 | GE | OMEGA | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| TORSION ANGLE MOLECULAR DYNAMICS | X-PLOR | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | NO EXPERIMENTAL DATA VIOLATIONS |
| Conformers Calculated Total Number | 80 |
| Conformers Submitted Total Number | 8 |
| Representative Model | 1 (n/a) |
| Additional NMR Experimental Information | |
|---|---|
| Details | FOR REPRESENTATIVE MODEL, SEE 2BJ2. THE STRUCTURE WAS DETERMINED USING DOUBLE AND TRIPLE RESONANCE NMR SPECTROSCOPY ON 13C, 15N LABELED RNA. |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | X-PLOR | 3.851 | BRUNGER |
| 2 | structure solution | X-PLOR | ||
