1B9U
MEMBRANE DOMAIN OF THE SUBUNIT B OF THE E.COLI ATP SYNTHASE
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | DQF-COSY | CHLOROFORM/METHANOL/WATER=4/4/1 | 50 mM NACL | 6.0 | 1 atm | 300 | ||
| 2 | TOCSY | CHLOROFORM/METHANOL/WATER=4/4/1 | 50 mM NACL | 6.0 | 1 atm | 300 | ||
| 3 | NOESY | CHLOROFORM/METHANOL/WATER=4/4/1 | 50 mM NACL | 6.0 | 1 atm | 300 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DMX | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | DYANA, DISCOVER (MSI) | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | LOWEST TARGET FUNCTION VALUE |
| Conformers Calculated Total Number | 200 |
| Conformers Submitted Total Number | 10 |
| Additional NMR Experimental Information | |
|---|---|
| Details | WATER SUPPESSION USING PULSED FIELD GRADIENTS |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | DYANA, DISCOVER (MSI) | 1.5 | GUENTERT ET AL. |
| 2 | structure solution | DYANA | 1.5 | |
