Annotations: 8HIJ

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Cytochromes	8057879	3001526	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF07361	e8hijA3	A: alpha bundles	X: Four-helical up-and-down bundle	H: Cytochromes (From Topology)	T: Cytochromes	F: PF07361	ECOD (1.6)
A	PF01770	e8hijA1	A: alpha complex topology	X: Major facilitator superfamily (MFS) general substrate transporter (From Topology)	H: Major facilitator superfamily (MFS) general substrate transporter (From Topology)	T: Major facilitator superfamily (MFS) general substrate transporter	F: PF01770	ECOD (1.6)
B [auth H]	PF07654	e8hijH2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF07654	ECOD (1.6)
B [auth H]	PF07686	e8hijH1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF07686	ECOD (1.6)
C [auth N]	PF07686	e8hijN1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF07686	ECOD (1.6)
D [auth L]	PF07654	e8hijL2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF07654	ECOD (1.6)
D [auth L]	PF07686	e8hijL1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF07686	ECOD (1.6)

SAbDab Antibody Annotation SAbDab Database Homepage

Chain	Chain Class	Chain Subclass	Chain Type	Antigen Name(s)	Provenance Source
B [auth H]	Heavy Chain	IGHV3	-	bril-slc19a1 chimera	SAbDab (2024-05-31)
C [auth N]	Heavy Chain	IGHV1	-	-	SAbDab (2024-05-31)
D [auth L]	Light Chain	IGKV1	Kappa	bril-slc19a1 chimera	SAbDab (2024-05-31)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF01770	Reduced folate carrier (Folate_carrier)	Reduced folate carrier	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	BRIL-SLC19A1 chimera	monoatomic anion transmembrane transporter activity folic acid transmembrane transporter activity vitamin transmembrane transporter activity amide transmembrane transporter activity carboxylic acid transmembrane transporter activity modified amino acid transmembrane transporter activity secondary active transmembrane transporter activity folate:monoatomic anion antiporter activity antiporter activity active monoatomic ion transmembrane transporter activity active transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity organic acid transmembrane transporter activity transmembrane transporter activity monoatomic anion transmembrane transporter activity folic acid transmembrane transporter activity vitamin transmembrane transporter activity amide transmembrane transporter activity carboxylic acid transmembrane transporter activity modified amino acid transmembrane transporter activity secondary active transmembrane transporter activity folate:monoatomic anion antiporter activity antiporter activity active monoatomic ion transmembrane transporter activity active transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity organic acid transmembrane transporter activity transmembrane transporter activity organic anion transmembrane transporter activity xenobiotic transmembrane transporter activity dicarboxylic acid transmembrane transporter activity methotrexate transmembrane transporter activity nucleotide transmembrane transporter activity guanine nucleotide transmembrane transporter activity purine ribonucleotide transmembrane transporter activity adenine nucleotide transmembrane transporter activity purine nucleotide transmembrane transporter activity carbohydrate derivative transmembrane transporter activity nucleobase-containing compound transmembrane transporter activity cyclic-GMP-AMP transmembrane transporter activity organophosphate ester transmembrane transporter activity cyclic GMP-AMP binding binding guanyl nucleotide binding adenyl ribonucleotide binding cyclic nucleotide binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding 2',3'-cyclic GMP-AMP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding amide binding organic acid binding modified amino acid binding vitamin binding folic acid binding carboxylic acid binding tetrapyrrole binding heme binding electron transfer activity cation binding transition metal ion binding iron ion binding metal ion binding	organic acid transmembrane transport import into cell organic anion transport nitrogen compound transport carboxylic acid transport carboxylic acid transmembrane transport transport organic substance transport vitamin transport import across plasma membrane folate transmembrane transport establishment of localization organic acid transport transmembrane transport localization organic acid transmembrane transport import into cell organic anion transport nitrogen compound transport carboxylic acid transport carboxylic acid transmembrane transport transport organic substance transport vitamin transport import across plasma membrane folate transmembrane transport establishment of localization organic acid transport transmembrane transport localization folate import across plasma membrane amide transport folic acid transport dicarboxylic acid transport cellular process modified amino acid transport vitamin transmembrane transport multi-multicellular organism process multicellular organismal process reproductive process female pregnancy multi-organism reproductive process xenobiotic transmembrane transport xenobiotic transport organonitrogen compound metabolic process oxoacid metabolic process cellular metabolic process dicarboxylic acid metabolic process carboxylic acid metabolic process organic acid metabolic process vitamin metabolic process cellular modified amino acid metabolic process organic cyclic compound metabolic process organic substance metabolic process folic acid-containing compound metabolic process small molecule metabolic process metabolic process amide metabolic process water-soluble vitamin metabolic process pteridine-containing compound metabolic process folic acid metabolic process guanine nucleotide transmembrane transport nucleotide transport carbohydrate derivative transport purine nucleotide transport adenine nucleotide transport cyclic-GMP-AMP transmembrane import across plasma membrane guanine nucleotide transport nucleobase-containing compound transport purine-containing compound transmembrane transport purine ribonucleotide transport nucleotide transmembrane transport cyclic nucleotide transport organophosphate ester transport system process circulatory system process vascular process in circulatory system vascular transport transport across blood-brain barrier methotrexate transport electron transport chain generation of precursor metabolites and energy	cell periphery apical part of cell membrane plasma membrane plasma membrane region cellular anatomical entity apical plasma membrane basal part of cell basal plasma membrane basolateral plasma membrane brush border membrane cluster of actin-based cell projections plasma membrane bounded cell projection brush border cell periphery apical part of cell membrane plasma membrane plasma membrane region cellular anatomical entity apical plasma membrane basal part of cell basal plasma membrane basolateral plasma membrane brush border membrane cluster of actin-based cell projections plasma membrane bounded cell projection brush border cell projection cell projection membrane periplasmic space
B [auth H]	Anti-BRIL Fab heavy chain	-	-	-
C [auth N]	Anti-Fab nanobody	-	-	-
D [auth L]	Anti-BRIL Fab light chain	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR002666	Reduced folate carrier	Family
A	IPR028339	Reduced folate transporter SLC19A1	Family
A	IPR036259	MFS transporter superfamily	Homologous Superfamily
A	IPR010980	Cytochrome c/b562	Homologous Superfamily
A	IPR009155	Cytochrome b562	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P41440	cleft palate Down syndrome malignant colon neoplasm urinary bladder cancer nervous system disorder follicular thyroid adenoma adenoma microcystic adenoma papillary adenoma colorectal cancer Crohn disease Down Syndrome, Partial Trisomy 21 familial hemolytic anemia malignant glioma mature T-cell and NK-cell non-Hodgkin lymphoma cleft palate Down syndrome malignant colon neoplasm urinary bladder cancer nervous system disorder follicular thyroid adenoma adenoma microcystic adenoma papillary adenoma colorectal cancer Crohn disease Down Syndrome, Partial Trisomy 21 familial hemolytic anemia malignant glioma mature T-cell and NK-cell non-Hodgkin lymphoma autism breast cancer ovarian cancer colorectal carcinoma tuberculosis urinary bladder neoplasm lung adenocarcinoma malignant mesothelioma medulloblastoma non-small cell lung carcinoma childhood acute lymphoblastic leukemia pancreatic intraductal papillary-mucinous neoplasm colonic neoplasm ulcerative colitis large cell medulloblastoma malignant pleural mesothelioma benign colon neoplasm osteosarcoma cleft lip psoriasis

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A	OPM	Transmembrane	Alpha-helical polytopic	Major Facilitator Superfamily (MFS)	Reduced folate carrier

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A	MemProtMD	Annotated as membrane protein by MemProtMD

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.