7MIB

Half integration complex of Cas4/Cas1/Cas2 with Cas4 still on the Non-PAM side

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B [auth A]	PF01867	e7mibA1	A: alpha complex topology	X: Cas1 (From Topology)	H: Cas1 (From Topology)	T: Cas1	F: PF01867	ECOD (1.6)
C [auth B]	PF01867	e7mibB1	A: alpha complex topology	X: Cas1 (From Topology)	H: Cas1 (From Topology)	T: Cas1	F: PF01867	ECOD (1.6)
E [auth D]	PF01867	e7mibD1	A: alpha complex topology	X: Cas1 (From Topology)	H: Cas1 (From Topology)	T: Cas1	F: PF01867	ECOD (1.6)
D [auth C]	PF01867	e7mibC1	A: alpha complex topology	X: Cas1 (From Topology)	H: Cas1 (From Topology)	T: Cas1	F: PF01867	ECOD (1.6)
D [auth C]	PF01930	e7mibC2	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: PF01930	ECOD (1.6)
F [auth E]	PF09827	e7mibE1	A: a+b two layers	X: Alpha-beta plaits	H: CRISPR associated protein Cas2-like (From Topology)	T: CRISPR associated protein Cas2-like	F: PF09827	ECOD (1.6)
G [auth F]	PF09827	e7mibF1	A: a+b two layers	X: Alpha-beta plaits	H: CRISPR associated protein Cas2-like (From Topology)	T: CRISPR associated protein Cas2-like	F: PF09827	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
B [auth A], C [auth B], D [auth C], E [auth D]	PF01867	CRISPR associated protein Cas1 (Cas_Cas1)	CRISPR associated protein Cas1	-	Family
B [auth A], C [auth B], D [auth C], E [auth D]	PF12705	PD-(D/E)XK nuclease superfamily (PDDEXK_1)	PD-(D/E)XK nuclease superfamily	Members of this family belong to the PD-(D/E)XK nuclease superfamily	Domain
B [auth A], C [auth B], D [auth C], E [auth D]	PF01930	Domain of unknown function DUF83 (Cas_Cas4)	Domain of unknown function DUF83	-	Family
F [auth E], G [auth F]	PF09827	CRISPR associated protein Cas2 (CRISPR_Cas2)	CRISPR associated protein Cas2	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth G]	DNA (31-MER)	-	-	-
B [auth A], C [auth B], D [auth C], E [auth D]	CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion	nuclease activity hydrolase activity, acting on ester bonds hydrolase activity exonuclease activity catalytic activity DNA binding binding organic cyclic compound binding nucleic acid binding endonuclease activity iron-sulfur cluster binding 4 iron, 4 sulfur cluster binding metal cluster binding small molecule binding nuclease activity hydrolase activity, acting on ester bonds hydrolase activity exonuclease activity catalytic activity DNA binding binding organic cyclic compound binding nucleic acid binding endonuclease activity iron-sulfur cluster binding 4 iron, 4 sulfur cluster binding metal cluster binding small molecule binding cation binding ion binding metal ion binding	maintenance of CRISPR repeat elements chromosome organization primary metabolic process DNA metabolic process maintenance of DNA repeat elements nucleic acid metabolic process cellular component organization nucleobase-containing compound metabolic process organic cyclic compound metabolic process organelle organization organic substance metabolic process cellular process metabolic process cellular component organization or biogenesis maintenance of CRISPR repeat elements chromosome organization primary metabolic process DNA metabolic process maintenance of DNA repeat elements nucleic acid metabolic process cellular component organization nucleobase-containing compound metabolic process organic cyclic compound metabolic process organelle organization organic substance metabolic process cellular process metabolic process cellular component organization or biogenesis macromolecule metabolic process biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to stress response to external biotic stimulus response to virus	-
F [auth E], G [auth F]	CRISPR-associated endoribonuclease Cas2	endonuclease activity nuclease activity hydrolase activity, acting on ester bonds catalytic activity, acting on RNA RNA endonuclease activity catalytic activity, acting on a nucleic acid RNA nuclease activity hydrolase activity catalytic activity cation binding ion binding binding metal ion binding small molecule binding	biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to stress response to external biotic stimulus response to virus maintenance of CRISPR repeat elements chromosome organization biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to stress response to external biotic stimulus response to virus maintenance of CRISPR repeat elements chromosome organization primary metabolic process DNA metabolic process maintenance of DNA repeat elements nucleic acid metabolic process cellular component organization nucleobase-containing compound metabolic process organic cyclic compound metabolic process organelle organization organic substance metabolic process cellular process metabolic process cellular component organization or biogenesis macromolecule metabolic process	-
H	DNA (64-MER)	-	-	-
I	DNA (5'-D(PCPGPGPAPAPAPAPGPAPGPCPC)-3')	-	-	-
J	DNA (45-MER)	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
B [auth A], C [auth B], D [auth C], E [auth D]	IPR002729	CRISPR-associated protein Cas1	Family
B [auth A], C [auth B], D [auth C], E [auth D]	IPR042206	CRISPR-associated endonuclease Cas1, C-terminal domain	Homologous Superfamily
B [auth A], C [auth B], D [auth C], E [auth D]	IPR042211	CRISPR-associated endonuclease Cas1, N-terminal domain	Homologous Superfamily
B [auth A], C [auth B], D [auth C], E [auth D]	IPR011604	PD-(D/E)XK endonuclease-like domain superfamily	Homologous Superfamily
B [auth A], C [auth B], D [auth C], E [auth D]	IPR022765	Dna2/Cas4, domain of unknown function DUF83	Domain
B [auth A], C [auth B], D [auth C], E [auth D]	IPR013343	CRISPR-associated protein Cas4	Family
F [auth E], G [auth F]	IPR019199	Virulence-associated protein D / CRISPR associated protein Cas2	Family
F [auth E], G [auth F]	IPR021127	CRISPR-associated endonuclease Cas2	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.