Annotations: 7BTQ

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
F	SCOP2B Superfamily	RecA-like P-loop NTPases	8091625	3002019	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Restriction endonuclease-like	8091621	3000104	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	HsdR C-terminal domain-like	8091622	3002528	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	RecA-like P-loop NTPases	8091624	3002019	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF12161	e7btqA2	A: alpha arrays	X: N-terminal helical domain in restriction-modification system methylation subunit-like	H: N-terminal helical domain in restriction-modification system methylation subunit (From Topology)	T: N-terminal helical domain in restriction-modification system methylation subunit	F: PF12161	ECOD (1.6)
A	PF02384	e7btqA1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: PF02384	ECOD (1.6)
D	PF12161	e7btqD2	A: alpha arrays	X: N-terminal helical domain in restriction-modification system methylation subunit-like	H: N-terminal helical domain in restriction-modification system methylation subunit (From Topology)	T: N-terminal helical domain in restriction-modification system methylation subunit	F: PF12161	ECOD (1.6)
D	PF02384	e7btqD1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: PF02384	ECOD (1.6)
E	PF01420	e7btqE1	A: a+b complex topology	X: DNA methylase specificity domain (From Topology)	H: DNA methylase specificity domain (From Topology)	T: DNA methylase specificity domain	F: PF01420	ECOD (1.6)
F	PF12008	e7btqF3	A: alpha arrays	X: HSDR subunit helical domain (From Topology)	H: HSDR subunit helical domain (From Topology)	T: HSDR subunit helical domain	F: PF12008	ECOD (1.6)
F	PF12008	e7btqF1	A: alpha complex topology	X: EcoR124I HsdR C-terminal domain (From Topology)	H: EcoR124I HsdR C-terminal domain (From Topology)	T: EcoR124I HsdR C-terminal domain	F: PF12008	ECOD (1.6)
F	PF18766	e7btqF2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF18766	ECOD (1.6)
F	PF22679	e7btqF4	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF22679	ECOD (1.6)
F	PF04313	e7btqF5	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: PF04313	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, D	PF12161	HsdM N-terminal domain (HsdM_N)	HsdM N-terminal domain	This domain is found at the N-terminus of the methylase subunit of Type I DNA methyltransferases. This domain family is found in bacteria and archaea, and is typically between 123 and 138 amino acids in length. The family is found in association with ...	This domain is found at the N-terminus of the methylase subunit of Type I DNA methyltransferases. This domain family is found in bacteria and archaea, and is typically between 123 and 138 amino acids in length. The family is found in association with Pfam:PF02384. Mutations in this region of EcoKI methyltransferase Swiss:P08957 abolish the normally strong preference of this system for methylating hemimethylated substrate [1]. The structure of this domain has been shown to be all alpha-helical.	Domain
E	PF01420	Type I restriction modification DNA specificity domain (Methylase_S)	Type I restriction modification DNA specificity domain	This domain is also known as the target recognition domain (TRD). Restriction-modification (R-M) systems protect a bacterial cell against invasion of foreign DNA by endonucleolytic cleavage of DNA that lacks a site specific modification. The host ge ...	This domain is also known as the target recognition domain (TRD). Restriction-modification (R-M) systems protect a bacterial cell against invasion of foreign DNA by endonucleolytic cleavage of DNA that lacks a site specific modification. The host genome is protected from cleavage by methylation of specific nucleotides in the target sites. In type I systems, both restriction and modification activities are present in one heteromeric enzyme complex composed of one DNA specificity subunit (this family), two modification (M) subunits and two restriction (R) subunits [2].	Domain
F	PF18766	SWI2/SNF2 ATPase (SWI2_SNF2)	SWI2/SNF2 ATPase	-	Family
F	PF04313	Type I restriction enzyme R protein N terminus (HSDR_N) (HSDR_N)	Type I restriction enzyme R protein N terminus (HSDR_N)	-	Family
F	PF12008	Type I restriction and modification enzyme - subunit R C terminal (EcoR124_C)	Type I restriction and modification enzyme - subunit R C terminal	This enzyme has been characterised and shown to belong to a new family of the type I class of restriction and modification enzymes. This family is involved in bacterial defence by making double strand breaks in specific double stranded DNA sequences, ...	This enzyme has been characterised and shown to belong to a new family of the type I class of restriction and modification enzymes. This family is involved in bacterial defence by making double strand breaks in specific double stranded DNA sequences, e.g. that of invading bacteriophages. EcoR124 is made up of three subunits, HsdR, HsdS and HsdM. The R subunit has ATPase and restriction endonuclease activity. This domain is the C terminal of the R subunit [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, D	Type I restriction enzyme EcoR124II M protein	DNA binding binding organic cyclic compound binding nucleic acid binding transferase activity, transferring one-carbon groups N-methyltransferase activity transferase activity methyltransferase activity catalytic activity DNA-methyltransferase activity catalytic activity, acting on DNA site-specific DNA-methyltransferase (adenine-specific) activity catalytic activity, acting on a nucleic acid S-adenosylmethionine-dependent methyltransferase activity	biological process involved in interspecies interaction between organisms response to stimulus DNA restriction-modification system clearance of foreign intracellular nucleic acids response to biotic stimulus nucleobase-containing compound metabolic process DNA modification organic substance metabolic process response to stress response to external biotic stimulus response to other organism defense response defense response to symbiont response to external stimulus biological process involved in interspecies interaction between organisms response to stimulus DNA restriction-modification system clearance of foreign intracellular nucleic acids response to biotic stimulus nucleobase-containing compound metabolic process DNA modification organic substance metabolic process response to stress response to external biotic stimulus response to other organism defense response defense response to symbiont response to external stimulus primary metabolic process clearance of foreign intracellular DNA DNA metabolic process nucleic acid metabolic process organic cyclic compound metabolic process defense response to other organism macromolecule modification metabolic process macromolecule metabolic process	-
B	DNA (64-MER)	-	-	-
C	DNA (64-MER)	-	-	-
E	Type-1 restriction enzyme EcoR124II specificity protein	DNA binding binding organic cyclic compound binding nucleic acid binding	biological process involved in interspecies interaction between organisms response to stimulus DNA restriction-modification system clearance of foreign intracellular nucleic acids response to biotic stimulus nucleobase-containing compound metabolic process DNA modification organic substance metabolic process response to stress response to external biotic stimulus response to other organism defense response defense response to symbiont response to external stimulus biological process involved in interspecies interaction between organisms response to stimulus DNA restriction-modification system clearance of foreign intracellular nucleic acids response to biotic stimulus nucleobase-containing compound metabolic process DNA modification organic substance metabolic process response to stress response to external biotic stimulus response to other organism defense response defense response to symbiont response to external stimulus primary metabolic process clearance of foreign intracellular DNA DNA metabolic process nucleic acid metabolic process organic cyclic compound metabolic process defense response to other organism macromolecule modification metabolic process macromolecule metabolic process	-
F	Type I restriction enzyme R Protein	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding DNA binding nucleic acid binding endonuclease activity catalytic activity, acting on a nucleic acid endodeoxyribonuclease activity, producing 5'-phosphomonoesters hydrolase activity DNA nuclease activity ATP-dependent activity ATP-dependent activity, acting on DNA restriction endodeoxyribonuclease activity DNA endonuclease activity type I site-specific deoxyribonuclease activity nuclease activity hydrolase activity, acting on ester bonds catalytic activity, acting on DNA DNA translocase activity endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters catalytic activity	biological process involved in interspecies interaction between organisms response to stimulus DNA restriction-modification system clearance of foreign intracellular nucleic acids response to biotic stimulus nucleobase-containing compound metabolic process DNA modification organic substance metabolic process response to stress response to external biotic stimulus response to other organism defense response defense response to symbiont response to external stimulus biological process involved in interspecies interaction between organisms response to stimulus DNA restriction-modification system clearance of foreign intracellular nucleic acids response to biotic stimulus nucleobase-containing compound metabolic process DNA modification organic substance metabolic process response to stress response to external biotic stimulus response to other organism defense response defense response to symbiont response to external stimulus primary metabolic process clearance of foreign intracellular DNA DNA metabolic process nucleic acid metabolic process organic cyclic compound metabolic process defense response to other organism macromolecule modification metabolic process macromolecule metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, D	IPR004546	Restriction endonuclease, type I, methylase subunit	Family
A, D	IPR002052	DNA methylase, N-6 adenine-specific, conserved site	Conserved Site
A, D	IPR003356	DNA methylase, adenine-specific	Domain
A, D	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase superfamily	Homologous Superfamily
A, D	IPR022749	N6 adenine-specific DNA methyltransferase, N-terminal domain	Domain
A, D	IPR038333	Type I restriction enzyme EcoKI-like, methylase subunit, N-terminal domain superfamily	Homologous Superfamily
E	IPR044946	Type I restriction modification DNA specificity domain superfamily	Homologous Superfamily
E	IPR000055	Type I restriction modification DNA specificity domain	Domain
F	IPR007409	Restriction endonuclease, type I, HsdR, N-terminal	Domain
F	IPR004473	Restriction endonuclease, type I, HsdR	Family
F	IPR040980	SWI2/SNF2 ATPase	Domain
F	IPR014001	Helicase superfamily 1/2, ATP-binding domain	Domain
F	IPR022625	Type I restriction enzyme R protein, C-terminal	Domain
F	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.