Annotations: 7AFT

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	SecY-like	8080650	3000333	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Protein translocase subunit SecE-like	8053310	3002174	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
F	F_UNCLASSIFIED	e7aftF1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: F_UNCLASSIFIED	ECOD (1.6)
A	PF00344,PF10559	e7aftA1	A: alpha complex topology	X: Preprotein translocase SecY subunit (From Topology)	H: Preprotein translocase SecY subunit (From Topology)	T: Preprotein translocase SecY subunit	F: PF00344,PF10559	ECOD (1.6)
B	PF03911	e7aftB1	A: extended segments	X: Sec-beta subunit (From Topology)	H: Sec-beta subunit (From Topology)	T: Sec-beta subunit	F: PF03911	ECOD (1.6)
C	PF00584	e7aftC1	A: extended segments	X: Preprotein translocase SecE subunit (From Topology)	H: Preprotein translocase SecE subunit (From Topology)	T: Preprotein translocase SecE subunit	F: PF00584	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF10559	Plug domain of Sec61p (Plug_translocon)	Plug domain of Sec61p	The Sec61/SecY translocon mediates translocation of proteins across the membrane and integration of membrane proteins into the lipid bilayer. The structure of the translocon revealed a plug domain blocking the pore on the lumenal side.The plug is unl ...	The Sec61/SecY translocon mediates translocation of proteins across the membrane and integration of membrane proteins into the lipid bilayer. The structure of the translocon revealed a plug domain blocking the pore on the lumenal side.The plug is unlikely to be important for sealing the translocation pore in yeast but it plays a role in stabilising Sec61p during translocon formation. The domain runs from residues 52-74 [1].	Domain
A	PF00344	SecY (SecY)	SecY	-	Family
B	PF03911	Sec61beta family (Sec61_beta)	Sec61beta family	-	Family
C	PF00584	SecE/Sec61-gamma subunits of protein translocation complex (SecE)	SecE/Sec61-gamma subunits of protein translocation complex	-	Family
E	PF09802	Preprotein translocase subunit Sec66 (Sec66)	Preprotein translocase subunit Sec66	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
F	Translocation protein SEC72	macromolecule transmembrane transporter activity protein transporter activity protein transmembrane transporter activity transporter activity transmembrane transporter activity	protein localization establishment of protein localization cellular localization nitrogen compound transport transport protein targeting localization protein transmembrane transport cellular macromolecule localization establishment of localization in cell post-translational protein targeting to membrane, translocation establishment of protein localization to endoplasmic reticulum protein localization to endoplasmic reticulum establishment of protein localization to membrane protein localization establishment of protein localization cellular localization nitrogen compound transport transport protein targeting localization protein transmembrane transport cellular macromolecule localization establishment of localization in cell post-translational protein targeting to membrane, translocation establishment of protein localization to endoplasmic reticulum protein localization to endoplasmic reticulum establishment of protein localization to membrane intracellular protein transmembrane transport organic substance transport intracellular transport post-translational protein targeting to endoplasmic reticulum membrane localization within membrane protein localization to membrane establishment of localization protein targeting to membrane transmembrane transport establishment of protein localization to organelle protein transport macromolecule localization protein localization to organelle cellular process intracellular protein transport protein targeting to ER	membrane-bounded organelle protein-containing complex intracellular anatomical structure organelle subcompartment membrane membrane protein complex cellular anatomical entity intracellular membrane-bounded organelle endomembrane system rough endoplasmic reticulum endoplasmic reticulum subcompartment intracellular organelle bounding membrane of organelle translocon complex membrane-bounded organelle protein-containing complex intracellular anatomical structure organelle subcompartment membrane membrane protein complex cellular anatomical entity intracellular membrane-bounded organelle endomembrane system rough endoplasmic reticulum endoplasmic reticulum subcompartment intracellular organelle bounding membrane of organelle translocon complex rough endoplasmic reticulum membrane organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane Sec62/Sec63 complex endoplasmic reticulum Sec complex
A	Protein transport protein SEC61	peptide transmembrane transporter activity transporter activity transmembrane transporter activity macromolecule transmembrane transporter activity protein transporter activity protein transmembrane transporter activity signal sequence binding binding peptide binding ribonucleoprotein complex binding protein-containing complex binding ribosome binding active transmembrane transporter activity primary active transmembrane transporter activity peptide transmembrane transporter activity transporter activity transmembrane transporter activity macromolecule transmembrane transporter activity protein transporter activity protein transmembrane transporter activity signal sequence binding binding peptide binding ribonucleoprotein complex binding protein-containing complex binding ribosome binding active transmembrane transporter activity primary active transmembrane transporter activity ATPase-coupled transmembrane transporter activity protein-transporting ATPase activity ATP-dependent activity	protein localization cellular localization establishment of protein localization nitrogen compound transport transport misfolded protein transport organic substance transport intracellular transport establishment of localization localization protein transport establishment of localization in cell cellular macromolecule localization macromolecule localization protein localization cellular localization establishment of protein localization nitrogen compound transport transport misfolded protein transport organic substance transport intracellular transport establishment of localization localization protein transport establishment of localization in cell cellular macromolecule localization macromolecule localization cellular process intracellular protein transport protein transmembrane import into intracellular organelle transmembrane transport establishment of protein localization to organelle protein transmembrane transport protein localization to organelle protein targeting post-translational protein targeting to membrane, translocation establishment of protein localization to endoplasmic reticulum protein localization to endoplasmic reticulum establishment of protein localization to membrane intracellular protein transmembrane transport post-translational protein targeting to endoplasmic reticulum membrane localization within membrane protein localization to membrane protein targeting to membrane protein targeting to ER cellular response to stimulus protein exit from endoplasmic reticulum response to organic substance catabolic process response to stimulus proteolysis involved in protein catabolic process macromolecule catabolic process ERAD pathway organic substance metabolic process cellular response to stress response to stress proteolysis protein metabolic process response to endoplasmic reticulum stress organonitrogen compound catabolic process organonitrogen compound metabolic process endoplasmic reticulum to cytosol transport response to organonitrogen compound response to nitrogen compound proteasomal protein catabolic process primary metabolic process protein catabolic process response to chemical retrograde protein transport, ER to cytosol metabolic process macromolecule metabolic process organic substance catabolic process SRP-dependent cotranslational protein targeting to membrane cotranslational protein targeting to membrane SRP-dependent cotranslational protein targeting to membrane, translocation	membrane-bounded organelle protein-containing complex intracellular anatomical structure Sec61 translocon complex membrane protein complex endomembrane system rough endoplasmic reticulum endoplasmic reticulum subcompartment intracellular organelle bounding membrane of organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane organelle subcompartment membrane-bounded organelle protein-containing complex intracellular anatomical structure Sec61 translocon complex membrane protein complex endomembrane system rough endoplasmic reticulum endoplasmic reticulum subcompartment intracellular organelle bounding membrane of organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane organelle subcompartment membrane cellular anatomical entity intracellular membrane-bounded organelle translocon complex rough endoplasmic reticulum membrane organelle endoplasmic reticulum cytoplasm organelle membrane
B	Protein transport protein SBH1	macromolecule transmembrane transporter activity protein transporter activity protein transmembrane transporter activity transporter activity transmembrane transporter activity GTPase regulator activity enzyme regulator activity guanyl-nucleotide exchange factor activity nucleoside-triphosphatase regulator activity molecular function regulator activity	protein localization establishment of protein localization cellular localization nitrogen compound transport transport protein targeting localization protein transmembrane transport cellular macromolecule localization establishment of localization in cell post-translational protein targeting to membrane, translocation establishment of protein localization to endoplasmic reticulum protein localization to endoplasmic reticulum establishment of protein localization to membrane protein localization establishment of protein localization cellular localization nitrogen compound transport transport protein targeting localization protein transmembrane transport cellular macromolecule localization establishment of localization in cell post-translational protein targeting to membrane, translocation establishment of protein localization to endoplasmic reticulum protein localization to endoplasmic reticulum establishment of protein localization to membrane intracellular protein transmembrane transport organic substance transport intracellular transport post-translational protein targeting to endoplasmic reticulum membrane localization within membrane protein localization to membrane establishment of localization protein targeting to membrane transmembrane transport establishment of protein localization to organelle protein transport macromolecule localization protein localization to organelle cellular process intracellular protein transport protein targeting to ER SRP-dependent cotranslational protein targeting to membrane cotranslational protein targeting to membrane SRP-dependent cotranslational protein targeting to membrane, translocation	membrane-bounded organelle protein-containing complex intracellular anatomical structure Sec61 translocon complex membrane protein complex endomembrane system rough endoplasmic reticulum endoplasmic reticulum subcompartment intracellular organelle bounding membrane of organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane organelle subcompartment membrane-bounded organelle protein-containing complex intracellular anatomical structure Sec61 translocon complex membrane protein complex endomembrane system rough endoplasmic reticulum endoplasmic reticulum subcompartment intracellular organelle bounding membrane of organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane organelle subcompartment membrane cellular anatomical entity intracellular membrane-bounded organelle translocon complex rough endoplasmic reticulum membrane organelle endoplasmic reticulum cytoplasm organelle membrane cytosol
C	Protein transport protein SSS1	macromolecule transmembrane transporter activity protein transporter activity protein transmembrane transporter activity transporter activity transmembrane transporter activity structural molecule activity	protein localization establishment of protein localization cellular localization establishment of protein localization to membrane protein targeting localization within membrane protein localization to membrane establishment of localization protein targeting to membrane localization SRP-dependent cotranslational protein targeting to membrane establishment of protein localization to organelle cellular macromolecule localization macromolecule localization protein localization to organelle protein localization establishment of protein localization cellular localization establishment of protein localization to membrane protein targeting localization within membrane protein localization to membrane establishment of localization protein targeting to membrane localization SRP-dependent cotranslational protein targeting to membrane establishment of protein localization to organelle cellular macromolecule localization macromolecule localization protein localization to organelle cotranslational protein targeting to membrane cellular process establishment of protein localization to endoplasmic reticulum protein localization to endoplasmic reticulum protein targeting to ER nitrogen compound transport transport protein transmembrane transport establishment of localization in cell post-translational protein targeting to membrane, translocation intracellular protein transmembrane transport organic substance transport intracellular transport post-translational protein targeting to endoplasmic reticulum membrane transmembrane transport protein transport intracellular protein transport SRP-dependent cotranslational protein targeting to membrane, translocation	membrane-bounded organelle protein-containing complex intracellular anatomical structure Sec61 translocon complex membrane protein complex endomembrane system rough endoplasmic reticulum endoplasmic reticulum subcompartment intracellular organelle bounding membrane of organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane organelle subcompartment membrane-bounded organelle protein-containing complex intracellular anatomical structure Sec61 translocon complex membrane protein complex endomembrane system rough endoplasmic reticulum endoplasmic reticulum subcompartment intracellular organelle bounding membrane of organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane organelle subcompartment membrane cellular anatomical entity intracellular membrane-bounded organelle translocon complex rough endoplasmic reticulum membrane organelle endoplasmic reticulum cytoplasm organelle membrane organelle inner membrane nuclear membrane nucleus organelle envelope nuclear inner membrane nuclear envelope Ssh1 translocon complex
D	Protein translocation protein SEC63	macromolecule transmembrane transporter activity protein transporter activity protein transmembrane transporter activity transporter activity transmembrane transporter activity	protein localization establishment of protein localization cellular localization establishment of protein localization to membrane protein targeting localization within membrane protein localization to membrane establishment of localization protein targeting to membrane localization SRP-dependent cotranslational protein targeting to membrane establishment of protein localization to organelle cellular macromolecule localization macromolecule localization protein localization to organelle protein localization establishment of protein localization cellular localization establishment of protein localization to membrane protein targeting localization within membrane protein localization to membrane establishment of localization protein targeting to membrane localization SRP-dependent cotranslational protein targeting to membrane establishment of protein localization to organelle cellular macromolecule localization macromolecule localization protein localization to organelle cotranslational protein targeting to membrane cellular process establishment of protein localization to endoplasmic reticulum protein localization to endoplasmic reticulum protein targeting to ER post-translational protein targeting to endoplasmic reticulum membrane transmembrane transport transport establishment of localization in cell intracellular transport cytosol to endoplasmic reticulum transport nitrogen compound transport protein transmembrane transport post-translational protein targeting to membrane, translocation intracellular protein transmembrane transport organic substance transport protein transport intracellular protein transport	membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle organelle inner membrane nuclear membrane membrane nucleus endomembrane system organelle envelope membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle organelle inner membrane nuclear membrane membrane nucleus endomembrane system organelle envelope nuclear inner membrane nuclear envelope organelle membrane protein-containing complex organelle subcompartment membrane protein complex rough endoplasmic reticulum endoplasmic reticulum subcompartment bounding membrane of organelle translocon complex rough endoplasmic reticulum membrane nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane Sec62/Sec63 complex endoplasmic reticulum Sec complex
E	Translocation protein SEC66	-	growth filamentous growth protein localization establishment of protein localization cellular localization nitrogen compound transport transport protein targeting localization protein transmembrane transport cellular macromolecule localization establishment of localization in cell post-translational protein targeting to membrane, translocation establishment of protein localization to endoplasmic reticulum growth filamentous growth protein localization establishment of protein localization cellular localization nitrogen compound transport transport protein targeting localization protein transmembrane transport cellular macromolecule localization establishment of localization in cell post-translational protein targeting to membrane, translocation establishment of protein localization to endoplasmic reticulum protein localization to endoplasmic reticulum establishment of protein localization to membrane intracellular protein transmembrane transport organic substance transport intracellular transport post-translational protein targeting to endoplasmic reticulum membrane localization within membrane protein localization to membrane establishment of localization protein targeting to membrane transmembrane transport establishment of protein localization to organelle protein transport macromolecule localization protein localization to organelle cellular process intracellular protein transport protein targeting to ER	cell periphery cellular anatomical entity membrane-bounded organelle protein-containing complex intracellular anatomical structure organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system rough endoplasmic reticulum endoplasmic reticulum subcompartment intracellular organelle bounding membrane of organelle cell periphery cellular anatomical entity membrane-bounded organelle protein-containing complex intracellular anatomical structure organelle subcompartment membrane membrane protein complex intracellular membrane-bounded organelle endomembrane system rough endoplasmic reticulum endoplasmic reticulum subcompartment intracellular organelle bounding membrane of organelle translocon complex rough endoplasmic reticulum membrane organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane Sec62/Sec63 complex endoplasmic reticulum Sec complex
G	Translocation protein SEC62,Translocation protein SEC62	macromolecule transmembrane transporter activity protein transporter activity protein transmembrane transporter activity transporter activity transmembrane transporter activity	protein localization establishment of protein localization cellular localization nitrogen compound transport transport protein targeting localization protein transmembrane transport cellular macromolecule localization establishment of localization in cell post-translational protein targeting to membrane, translocation establishment of protein localization to endoplasmic reticulum protein localization to endoplasmic reticulum establishment of protein localization to membrane protein localization establishment of protein localization cellular localization nitrogen compound transport transport protein targeting localization protein transmembrane transport cellular macromolecule localization establishment of localization in cell post-translational protein targeting to membrane, translocation establishment of protein localization to endoplasmic reticulum protein localization to endoplasmic reticulum establishment of protein localization to membrane intracellular protein transmembrane transport organic substance transport intracellular transport post-translational protein targeting to endoplasmic reticulum membrane localization within membrane protein localization to membrane establishment of localization protein targeting to membrane transmembrane transport establishment of protein localization to organelle protein transport macromolecule localization protein localization to organelle cellular process intracellular protein transport protein targeting to ER	membrane-bounded organelle protein-containing complex intracellular anatomical structure organelle subcompartment membrane membrane protein complex cellular anatomical entity intracellular membrane-bounded organelle endomembrane system rough endoplasmic reticulum endoplasmic reticulum subcompartment intracellular organelle bounding membrane of organelle translocon complex membrane-bounded organelle protein-containing complex intracellular anatomical structure organelle subcompartment membrane membrane protein complex cellular anatomical entity intracellular membrane-bounded organelle endomembrane system rough endoplasmic reticulum endoplasmic reticulum subcompartment intracellular organelle bounding membrane of organelle translocon complex rough endoplasmic reticulum membrane organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum protein-containing complex endoplasmic reticulum membrane Sec62/Sec63 complex endoplasmic reticulum Sec complex
H	Mating factor alpha-1,Mating factor alpha-1	cation binding cupric ion binding transition metal ion binding copper ion binding ion binding binding metal ion binding small molecule binding pheromone activity protein binding molecular function activator activity signaling receptor binding mating pheromone activity molecular function regulator activity cation binding cupric ion binding transition metal ion binding copper ion binding ion binding binding metal ion binding small molecule binding pheromone activity protein binding molecular function activator activity signaling receptor binding mating pheromone activity molecular function regulator activity signaling receptor regulator activity receptor ligand activity signaling receptor activator activity	sexual reproduction reproductive process mating multi-organism reproductive process cellular response to stimulus response to organic substance regulation of cellular process response to stimulus signaling regulation of reproductive process positive regulation of biological process regulation of conjugation with cellular fusion response to pheromone cellular response to pheromone sexual reproduction reproductive process mating multi-organism reproductive process cellular response to stimulus response to organic substance regulation of cellular process response to stimulus signaling regulation of reproductive process positive regulation of biological process regulation of conjugation with cellular fusion response to pheromone cellular response to pheromone cell communication positive regulation of conjugation with cellular fusion cellular response to organic substance signal transduction positive regulation of reproductive process pheromone-dependent signal transduction involved in conjugation with cellular fusion regulation of biological process signal transduction involved in positive regulation of conjugation with cellular fusion biological regulation conjugation with cellular fusion response to pheromone triggering conjugation with cellular fusion cellular response to chemical stimulus response to chemical cellular process	extracellular region cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
F	IPR011990	Tetratricopeptide-like helical domain superfamily	Homologous Superfamily
F	IPR019734	Tetratricopeptide repeat	Repeat
A	IPR002208	SecY/SEC61-alpha family	Family
A	IPR030659	SecY conserved site	Conserved Site
A	IPR023201	SecY domain superfamily	Homologous Superfamily
A	IPR019561	Translocon Sec61/SecY, plug domain	Domain
B	IPR030671	Protein transport Sec61-beta/Sbh	Family
B	IPR016482	Protein transport protein SecG/Sec61-beta/Sbh	Family
C	IPR008158	Protein translocase SEC61 complex, gamma subunit	Family
C	IPR001901	Protein translocase complex, SecE/Sec61-gamma subunit	Family
C	IPR023391	Protein translocase SecE domain superfamily	Homologous Superfamily
D	IPR018253	DnaJ domain, conserved site	Conserved Site
D	IPR036869	Chaperone J-domain superfamily	Homologous Superfamily
D	IPR004179	Sec63 domain	Domain
D	IPR001623	DnaJ domain	Domain
D	IPR014756	Immunoglobulin E-set	Homologous Superfamily
E	IPR018624	Translocation protein Sec66	Family
G	IPR011553	Translocation protein Sec62, ascomycota	Family
G	IPR004728	Translocation protein Sec62	Family
H	IPR006742	Mating factor alpha, C-terminal repeat	Repeat
H	IPR008675	Mating factor alpha precursor, N-terminal	Domain

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B, C, D, E A, B, C, D, E, G, H	OPM	Transmembrane	Alpha-helical polytopic	General secretory pathway (Sec)	Protein translocase

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B, C, D, E A, B, C, D, E, G, H	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A, B, C, D, E A, B, C, D, E, G, H	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	Sec, Translocase, and Insertase Proteins	Sec protein-translocation channel complex (Sec61-Sec63-Sec71-Sec72) post-translational complex