6U8Y

Structure of the membrane-bound sulfane sulfur reductase (MBS), an archaeal respiratory membrane complex

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
K [auth L]	SCOP2B Superfamily	HydB/Nqo4-like	8097970	3001849	SCOP2B (2022-06-29)
X [auth l]	SCOP2 Family	Nqo4-like	8097969	4000433	SCOP2 (2022-06-29)
X [auth l]	SCOP2 Superfamily	HydB/Nqo4-like	8097970	3001849	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	NADH-quinone oxidoreductase subunit 11-like	8097968	3002200	SCOP2B (2022-06-29)
O [auth b]	SCOP2 Family	Monovalent cation/H+ antiporter MBH subunit F	8097967	4007804	SCOP2 (2022-06-29)
O [auth b]	SCOP2 Superfamily	NADH-quinone oxidoreductase subunit 11-like	8097968	3002200	SCOP2 (2022-06-29)
C	SCOP2B Superfamily	NADH-quinone oxidoreductase subunit 11-like	8097962	3002200	SCOP2B (2022-06-29)
P [auth c]	SCOP2 Family	Monovalent cation/H+ antiporter MBH subunit G	8097961	4007805	SCOP2 (2022-06-29)
P [auth c]	SCOP2 Superfamily	NADH-quinone oxidoreductase subunit 11-like	8097962	3002200	SCOP2 (2022-06-29)
F [auth G]	SCOP2B Superfamily	NADH-quinone oxidoreductase subunit 11-like	8097964	3002200	SCOP2B (2022-06-29)
S [auth g]	SCOP2 Family	NADH-quinone oxidoreductase NDH subunit 11-like	8097963	4005793	SCOP2 (2022-06-29)
S [auth g]	SCOP2 Superfamily	NADH-quinone oxidoreductase subunit 11-like	8097964	3002200	SCOP2 (2022-06-29)
I [auth J]	SCOP2B Superfamily	Nqo6/HydA N-terminal domain-like	8097966	3002291	SCOP2B (2022-06-29)
V [auth j]	SCOP2 Family	Nq06/HydA N-terminal domain-like	8097965	4007641	SCOP2 (2022-06-29)
V [auth j]	SCOP2 Superfamily	Nqo6/HydA N-terminal domain-like	8097966	3002291	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
J [auth K]	PF00329	e6u8yK1	A: a+b two layers	X: Nqo5-like	H: Nqo5-like (From Topology)	T: Nqo5-like	F: PF00329	ECOD (1.6)
W [auth k]	PF00329	e6u8yk1	A: a+b two layers	X: Nqo5-like	H: Nqo5-like (From Topology)	T: Nqo5-like	F: PF00329	ECOD (1.6)
K [auth L]	PF00346	e6u8yL1	A: alpha complex topology	X: alpha-helical domain in nickel-iron hydrogenase, large subunit (From Topology)	H: alpha-helical domain in nickel-iron hydrogenase, large subunit (From Topology)	T: alpha-helical domain in nickel-iron hydrogenase, large subunit	F: PF00346	ECOD (1.6)
K [auth L]	PF00346	e6u8yL2	A: a+b two layers	X: FAD-linked reductases, C-terminal domain-like	H: Ni-Fe binding domain in nickel-iron hydrogenase large subunit (From Topology)	T: Ni-Fe binding domain in nickel-iron hydrogenase large subunit	F: PF00346	ECOD (1.6)
X [auth l]	PF00346	e6u8yl3	A: alpha complex topology	X: alpha-helical domain in nickel-iron hydrogenase, large subunit (From Topology)	H: alpha-helical domain in nickel-iron hydrogenase, large subunit (From Topology)	T: alpha-helical domain in nickel-iron hydrogenase, large subunit	F: PF00346	ECOD (1.6)
X [auth l]	PF00346	e6u8yl2	A: a+b two layers	X: FAD-linked reductases, C-terminal domain-like	H: Ni-Fe binding domain in nickel-iron hydrogenase large subunit (From Topology)	T: Ni-Fe binding domain in nickel-iron hydrogenase large subunit	F: PF00346	ECOD (1.6)
L [auth M]	PF00146	e6u8yM1	A: alpha complex topology	X: Sodium/proton antiporter subunits-like (From Homology)	H: Sodium/proton antiporter subunits-like	T: NADH-quinone oxidoreductase subunit 8 (Nqo8)-related	F: PF00146	ECOD (1.6)
Y [auth m]	PF00146	e6u8ym1	A: alpha complex topology	X: Sodium/proton antiporter subunits-like (From Homology)	H: Sodium/proton antiporter subunits-like	T: NADH-quinone oxidoreductase subunit 8 (Nqo8)-related	F: PF00146	ECOD (1.6)
F [auth G]	PF00420	e6u8yG1	A: alpha complex topology	X: NADH-quinone oxidoreductase subunit K (From Topology)	H: NADH-quinone oxidoreductase subunit K (From Topology)	T: NADH-quinone oxidoreductase subunit K	F: PF00420	ECOD (1.6)
S [auth g]	PF00420	e6u8yg1	A: alpha complex topology	X: NADH-quinone oxidoreductase subunit K (From Topology)	H: NADH-quinone oxidoreductase subunit K (From Topology)	T: NADH-quinone oxidoreductase subunit K	F: PF00420	ECOD (1.6)
G [auth H]	PF00361	e6u8yH1	A: alpha complex topology	X: Sodium/proton antiporter subunits-like (From Homology)	H: Sodium/proton antiporter subunits-like	T: NADH-quinone oxidoreductase subunit L (NuoL)-related	F: PF00361	ECOD (1.6)
T [auth h]	PF00361	e6u8yh1	A: alpha complex topology	X: Sodium/proton antiporter subunits-like (From Homology)	H: Sodium/proton antiporter subunits-like	T: NADH-quinone oxidoreductase subunit L (NuoL)-related	F: PF00361	ECOD (1.6)
H [auth X]	PF00361	e6u8yX1	A: alpha complex topology	X: Sodium/proton antiporter subunits-like (From Homology)	H: Sodium/proton antiporter subunits-like	T: NADH-quinone oxidoreductase subunit L (NuoL)-related	F: PF00361	ECOD (1.6)
U [auth x]	PF00361	e6u8yx1	A: alpha complex topology	X: Sodium/proton antiporter subunits-like (From Homology)	H: Sodium/proton antiporter subunits-like	T: NADH-quinone oxidoreductase subunit L (NuoL)-related	F: PF00361	ECOD (1.6)
I [auth J]	PF01058	e6u8yJ1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: CheY-like	F: PF01058	ECOD (1.6)
V [auth j]	PF01058	e6u8yj1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: CheY-like	F: PF01058	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, N [auth a]	PF01899	Na+/H+ ion antiporter subunit (MNHE)	Na+/H+ ion antiporter subunit	-	Family
J [auth K], W [auth k]	PF00329	Respiratory-chain NADH dehydrogenase, 30 Kd subunit (Complex1_30kDa)	Respiratory-chain NADH dehydrogenase, 30 Kd subunit	-	Family
K [auth L], X [auth l]	PF00346	Respiratory-chain NADH dehydrogenase, 49 Kd subunit (Complex1_49kDa)	Respiratory-chain NADH dehydrogenase, 49 Kd subunit	-	Family
L [auth M], Y [auth m]	PF00146	NADH dehydrogenase (NADHdh)	NADH dehydrogenase	-	Family
M [auth N], Z [auth n]	PF12838	4Fe-4S dicluster domain (Fer4_7)	4Fe-4S dicluster domain	Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contai ...	Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contains two 4Fe4S clusters.	Domain
B, O [auth b]	PF04066	Multiple resistance and pH regulation protein F (MrpF / PhaF) (MrpF_PhaF)	Multiple resistance and pH regulation protein F (MrpF / PhaF)	-	Family
C, P [auth c]	PF03334	Na+/H+ antiporter subunit (PhaG_MnhG_YufB)	Na+/H+ antiporter subunit	-	Family
D, Q [auth d]	PF13244	MBH, subunit D (MbhD)	MBH, subunit D	Hydrogen gas-evolving membrane-bound hydrogenase (MBH) is a respiratory complex homologous to the quinone-reducing Complex I. Like Complex I, MBH has peripheral and membrane arms. MBH is made of 14 subunits (MbhA-N). MbhJ, K, L, N and M form the Mem ...	Hydrogen gas-evolving membrane-bound hydrogenase (MBH) is a respiratory complex homologous to the quinone-reducing Complex I. Like Complex I, MBH has peripheral and membrane arms. MBH is made of 14 subunits (MbhA-N). MbhJ, K, L, N and M form the Membrane-anchored hydrogenase module. MbhJ, K, L, N are predicted to be exposed to the cytoplasm and form the peripheral arm. The remaining 10 subunits are predicted to be integral membrane proteins forming the membrane arm, made of 44 transmembrane helices (TMH) [2, 3]. MbhA, B, C and F form the Sodium translocation module. MbhD, E, G and H form the Proton translocation module. MbhI is the linker between the hydrogenase module and the proton-translocating membrane module. It anchors the discontinuous TMH7 of MbhH via its middle lateral helix and the C-terminal of TMH2, found in MbhE. MbhD and MbhE together are equivalent to Nqo10 of Complex I [1]. MbhD has three TM helices.	Domain
E, R [auth e]	PF04039	Domain related to MnhB subunit of Na+/H+ antiporter (MnhB)	Domain related to MnhB subunit of Na+/H+ antiporter	-	Family
E, R [auth e]	PF20501	MBH, subunit E (MbhE)	MBH, subunit E	Hydrogen gas-evolving membrane-bound hydrogenase (MBH) is a respiratory complex homologous to the quinone-reducing Complex I. Like Complex I, MBH has peripheral and membrane arms. MBH is made of 14 subunits (MbhA-N). MbhJ, K, L, N and M form the Mem ...	Hydrogen gas-evolving membrane-bound hydrogenase (MBH) is a respiratory complex homologous to the quinone-reducing Complex I. Like Complex I, MBH has peripheral and membrane arms. MBH is made of 14 subunits (MbhA-N). MbhJ, K, L, N and M form the Membrane-anchored hydrogenase module. MbhJ, K, L, N are predicted to be exposed to the cytoplasm and form the peripheral arm. The remaining 10 subunits are predicted to be integral membrane proteins forming the membrane arm, made of 44 transmembrane helices (TMH) [2, 3]. MbhA, B, C and F form the Sodium translocation module. MbhD, E, G and H form the Proton translocation module. MbhI is the linker between the hydrogenase module and the proton-translocating membrane module. It anchors the discontinuous TMH7 of MbhH via its middle lateral helix and the C-terminal of TMH2, found in MbhE. MbhD and MbhE together are equivalent to Nqo10 of Complex I [1]. MbhE has two transmembrane helices: TMH1 and TMH2.	Domain
F [auth G], S [auth g]	PF00420	NADH-ubiquinone/plastoquinone oxidoreductase chain 4L (Oxidored_q2)	NADH-ubiquinone/plastoquinone oxidoreductase chain 4L	-	Family
G [auth H], T [auth h]	PF00361	Proton-conducting membrane transporter (Proton_antipo_M)	Proton-conducting membrane transporter	-	Family
H [auth X], U [auth x]	PF00361	Proton-conducting membrane transporter (Proton_antipo_M)	Proton-conducting membrane transporter	-	Family
I [auth J], V [auth j]	PF01058	NADH ubiquinone oxidoreductase, 20 Kd subunit (Oxidored_q6)	NADH ubiquinone oxidoreductase, 20 Kd subunit	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, N [auth a]	Monovalent cation/H+ antiporter subunit E	monoatomic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity	-	cell periphery plasma membrane membrane cellular anatomical entity
J [auth K], W [auth k]	NADH dehydrogenase subunit C	monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity oxidoreductase activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity oxidoreductase activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity catalytic activity	-	-
K [auth L], X [auth l]	NADH dehydrogenase subunit D	NAD binding binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding adenyl nucleotide binding nucleotide binding heterocyclic compound binding small molecule binding oxidoreductase activity oxidoreductase activity, acting on NAD(P)H catalytic activity quinone binding	-	-
L [auth M], Y [auth m]	NADH dehydrogenase subunit	-	-	membrane cellular anatomical entity
M [auth N], Z [auth n]	NADH dehydrogenase subunit I	oxidoreductase activity oxidoreductase activity, acting on NAD(P)H catalytic activity iron-sulfur cluster binding binding 4 iron, 4 sulfur cluster binding metal cluster binding small molecule binding	-	membrane cellular anatomical entity
B, O [auth b]	Monovalent cation/H+ antiporter subunit F	monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity	-	cell periphery plasma membrane membrane cellular anatomical entity
C, P [auth c]	Monovalent cation/H+ antiporter subunit G	antiporter activity active transmembrane transporter activity transporter activity transmembrane transporter activity secondary active transmembrane transporter activity	transmembrane transport localization transport cellular process inorganic ion transmembrane transport establishment of localization inorganic cation transmembrane transport	membrane cellular anatomical entity
D, Q [auth d]	DUF4040 domain-containing protein	-	-	cell periphery plasma membrane membrane cellular anatomical entity
E, R [auth e]	Monovalent cation/H+ antiporter subunit B	-	-	cell periphery plasma membrane membrane cellular anatomical entity
F [auth G], S [auth g]	Monovalent cation/H+ antiporter subunit C	-	-	cell periphery plasma membrane membrane cellular anatomical entity
G [auth H], T [auth h]	NADH dehydrogenase subunit N	-	-	membrane cellular anatomical entity
H [auth X], U [auth x]	NADH dehydrogenase subunit M	-	-	membrane cellular anatomical entity
I [auth J], V [auth j]	NADH dehydrogenase subunit B	iron-sulfur cluster binding binding 4 iron, 4 sulfur cluster binding metal cluster binding small molecule binding cation binding ion binding metal ion binding monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity iron-sulfur cluster binding binding 4 iron, 4 sulfur cluster binding metal cluster binding small molecule binding cation binding ion binding metal ion binding monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity oxidoreductase activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity catalytic activity quinone binding	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, N [auth a]	IPR002758	Na+/H+ antiporter subunit E	Family
J [auth K], W [auth k]	IPR037232	NADH:ubiquinone oxidoreductase, 30kDa subunit superfamily	Homologous Superfamily
J [auth K], W [auth k]	IPR001268	NADH:ubiquinone oxidoreductase, 30kDa subunit	Domain
K [auth L], X [auth l]	IPR022885	NAD(P)H-quinone oxidoreductase subunit D/H	Family
K [auth L], X [auth l]	IPR014029	NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site	Conserved Site
K [auth L], X [auth l]	IPR029014	[NiFe]-hydrogenase, large subunit	Homologous Superfamily
K [auth L], X [auth l]	IPR001135	NADH-quinone oxidoreductase, subunit D	Domain
L [auth M], Y [auth m]	IPR001694	NADH:ubiquinone oxidoreductase, subunit 1/F420H2 oxidoreductase subunit H	Family
M [auth N], Z [auth n]	IPR017900	4Fe-4S ferredoxin, iron-sulphur binding, conserved site	Conserved Site
M [auth N], Z [auth n]	IPR017896	4Fe-4S ferredoxin-type, iron-sulphur binding domain	Domain
M [auth N], Z [auth n]	IPR010226	NADH-quinone oxidoreductase, chain I	Family
B, O [auth b]	IPR007208	Na(+)/H(+) antiporter subunit F-like	Family
C, P [auth c]	IPR005133	Na+/H+ antiporter subunit G	Family
D, Q [auth d]	IPR025383	MrpA C-terminal/MbhD	Domain
D, Q [auth d]	IPR042106	NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ	Homologous Superfamily
E, R [auth e]	IPR007182	Na+/H+ antiporter MnhB subunit-related protein	Domain
E, R [auth e]	IPR046806	MrpA C-terminal/MbhE	Domain
F [auth G], S [auth g]	IPR039428	NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like	Family
G [auth H], T [auth h]	IPR001750	NADH:quinone oxidoreductase/Mrp antiporter, membrane subunit	Domain
H [auth X], U [auth x]	IPR001750	NADH:quinone oxidoreductase/Mrp antiporter, membrane subunit	Domain
I [auth J], V [auth j]	IPR006138	NADH-ubiquinone oxidoreductase, 20 Kd subunit	Family
I [auth J], V [auth j]	IPR006137	NADH:ubiquinone oxidoreductase-like, 20kDa subunit	Domain

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B, C, D, E A, B, C, D, E, F [auth G], G [auth H], H [auth X], L [auth M], N [auth a], O [auth b], P [auth c], Q [auth d], R [auth e], S [auth g], T [auth h], U [auth x], Y [auth m]	OPM	Transmembrane	Alpha-helical polytopic	Electron transport chain complex I (NADH dehydrogenase) and supercomplexes	H+ or Na+ translocating NADH dehydrogenase

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B, C, D, E A, B, C, D, E, F [auth G], G [auth H], H [auth X], L [auth M], N [auth a], O [auth b], P [auth c], Q [auth d], R [auth e], S [auth g], T [auth h], U [auth x], Y [auth m]	PDBTM	Annotated as membrane protein by PDBTM

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6U8Y

Structure of the membrane-bound sulfane sulfur reductase (MBS), an archaeal respiratory membrane complex

External Resource: Annotation

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Domain Annotation: ECOD Classification ECOD Database Homepage

Protein Family Annotation Pfam Database Homepage

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

InterPro: Protein Family Classification InterPro Database Homepage

Membrane Protein Annotation: OPM OPM Database Homepage

Membrane Protein Annotation: PDBTM PDBTM Database Homepage