6PEK

Structure of Spastin Hexamer (Subunit A-E) in complex with substrate peptide

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C	PF09336,PF17862	e6pekC2	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: PF09336,PF17862	ECOD (1.6)
C	PF00004	e6pekC1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00004	ECOD (1.6)
A	PF09336,PF17862	e6pekA1	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: PF09336,PF17862	ECOD (1.6)
A	PF00004	e6pekA2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00004	ECOD (1.6)
B	PF09336,PF17862	e6pekB2	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: PF09336,PF17862	ECOD (1.6)
B	PF00004	e6pekB1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00004	ECOD (1.6)
D	PF09336,PF17862	e6pekD2	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: PF09336,PF17862	ECOD (1.6)
D	PF00004	e6pekD1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00004	ECOD (1.6)
E	PF17862	e6pekE2	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: PF17862	ECOD (1.6)
E	PF00004	e6pekE1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00004	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E	PF09336	Vps4 C terminal oligomerisation domain (Vps4_C)	Vps4 C terminal oligomerisation domain	This domain is found at the C terminal of ATPase proteins involved in vacuolar sorting. It forms an alpha helix structure and is required for oligomerisation [1].	Domain
A, B, C, D, E	PF17862	AAA+ lid domain (AAA_lid_3)	AAA+ lid domain	This entry represents the alpha helical AAA+ lid domain that is found to the C-terminus of AAA domains.	Domain
A, B, C, D, E	PF00004	ATPase family associated with various cellular activities (AAA) (AAA)	ATPase family associated with various cellular activities (AAA)	AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E	Spastin	cytoskeletal protein binding protein binding binding tubulin binding alpha-tubulin binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding cytoskeletal protein binding protein binding binding tubulin binding alpha-tubulin binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding microtubule binding protein-containing complex binding protein-containing complex destabilizing activity microtubule severing ATPase activity catalytic activity, acting on a protein catalytic activity ATP-dependent activity isomerase activity pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides beta-tubulin binding	regulation of cytokinesis regulation of biological process regulation of cellular process positive regulation of cell division regulation of cell cycle process biological regulation positive regulation of biological process cytokinesis positive regulation of cell cycle regulation of cell division positive regulation of cytokinesis positive regulation of cell cycle process cellular process cell division regulation of cytokinesis regulation of biological process regulation of cellular process positive regulation of cell division regulation of cell cycle process biological regulation positive regulation of biological process cytokinesis positive regulation of cell cycle regulation of cell division positive regulation of cytokinesis positive regulation of cell cycle process cellular process cell division cell cycle regulation of cell cycle positive regulation of cellular process cell cycle process cellular component organization organelle organization microtubule severing microtubule cytoskeleton organization microtubule-based process cellular component organization or biogenesis cytoskeleton organization cellular component disassembly spindle disassembly mitotic cell cycle mitotic spindle organization mitotic cell cycle process organelle disassembly mitotic spindle disassembly microtubule cytoskeleton organization involved in mitosis spindle organization membrane organization membrane biogenesis nuclear membrane reassembly membrane assembly nuclear membrane organization cellular component biogenesis nucleus organization cellular component assembly nuclear envelope organization endomembrane system organization cellular localization anterograde axonal transport transport intracellular transport axonal transport establishment of localization localization axo-dendritic transport establishment of localization in cell microtubule-based transport transport along microtubule cytoskeleton-dependent intracellular transport microtubule-based movement endoplasmic reticulum to Golgi vesicle-mediated transport Golgi vesicle transport vesicle-mediated transport multicellular organismal process axonogenesis cellular developmental process cell projection organization plasma membrane bounded cell projection morphogenesis cell development neuron projection development cell morphogenesis neurogenesis multicellular organism development cell projection morphogenesis neuron differentiation neuron projection morphogenesis cell differentiation anatomical structure development system development developmental process plasma membrane bounded cell projection organization nervous system development axon development cell morphogenesis involved in neuron differentiation generation of neurons anatomical structure morphogenesis neuron development establishment of organelle localization organelle transport along microtubule establishment of mitochondrion localization mitochondrion localization establishment of mitochondrion localization, microtubule-mediated mitochondrion transport along microtubule organelle localization axonal transport of mitochondrion microtubule bundle formation cytoskeleton-dependent cytokinesis mitotic cytokinesis protein hexamerization protein-containing complex organization protein complex oligomerization protein-containing complex assembly metabolic process mitotic nuclear division organelle fission cell cycle phase transition exit from mitosis nuclear division mitotic cell cycle phase transition cytokinetic process mitotic nuclear membrane reassembly mitotic nuclear membrane organization membrane fission positive regulation of organelle organization regulation of cytoskeleton organization regulation of microtubule polymerization or depolymerization regulation of cellular component organization positive regulation of cellular component organization protein-containing complex disassembly positive regulation of microtubule depolymerization regulation of protein-containing complex disassembly microtubule polymerization or depolymerization regulation of microtubule depolymerization positive regulation of protein-containing complex disassembly positive regulation of microtubule polymerization or depolymerization regulation of protein depolymerization positive regulation of protein depolymerization positive regulation of cytoskeleton organization regulation of microtubule-based process positive regulation of supramolecular fiber organization regulation of organelle organization microtubule depolymerization regulation of supramolecular fiber organization supramolecular fiber organization protein depolymerization regulation of microtubule cytoskeleton organization protein homooligomerization	intracellular organelle intracellular anatomical structure cytoskeleton spindle organelle intracellular non-membrane-bounded organelle spindle pole cellular anatomical entity microtubule cytoskeleton non-membrane-bounded organelle membrane-bounded organelle nuclear membrane membrane nucleus intracellular organelle intracellular anatomical structure cytoskeleton spindle organelle intracellular non-membrane-bounded organelle spindle pole cellular anatomical entity microtubule cytoskeleton non-membrane-bounded organelle membrane-bounded organelle nuclear membrane membrane nucleus intracellular membrane-bounded organelle endomembrane system organelle envelope nuclear envelope organelle membrane extracellular exosome extracellular vesicle extracellular organelle extracellular region extracellular space vesicle extracellular membrane-bounded organelle cytoplasm cytosol perinuclear region of cytoplasm axon cell projection neuron projection plasma membrane bounded cell projection polymeric cytoskeletal fiber supramolecular fiber microtubule supramolecular polymer supramolecular complex neuron projection cytoplasm axon cytoplasm cytoplasmic region plasma membrane bounded cell projection cytoplasm midbody centrosome microtubule organizing center membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen organelle subcompartment endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum endoplasmic reticulum membrane cytoplasmic vesicle intracellular vesicle endoplasmic reticulum tubular network endosome lipid droplet
F [auth G]	substrate peptide, TYR-GLU-TYR-GLU-TYR-GLU-TYR-GLU	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E	IPR003959	ATPase, AAA-type, core	Domain
A, B, C, D, E	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A, B, C, D, E	IPR007330	MIT domain	Domain
A, B, C, D, E	IPR017179	Spastin	Family
A, B, C, D, E	IPR003593	AAA+ ATPase domain	Domain
A, B, C, D, E	IPR041569	AAA ATPase, AAA+ lid domain	Domain
A, B, C, D, E	IPR035106	Spastin, chordate	Family
A, B, C, D, E	IPR015415	Spastin/Vps4, C-terminal	Domain
A, B, C, D, E	IPR003960	ATPase, AAA-type, conserved site	Conserved Site

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C, D, E	Pharos: Q9UBP0	hereditary spastic paraplegia 4 hereditary spastic paraplegia paraplegia Neurodevelopmental Disorders adenoma leukodystrophy medulloblastoma non-small cell lung carcinoma Sjogren syndrome Charcot-Marie-Tooth disease lung cancer neurodegenerative disease Gaucher disease intellectual disability primitive neuroectodermal tumor

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.