6MX6

The Prp8 intein of Cryptococcus neoformans

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF05203	e6mx6A1	A: beta complex topology	X: Hedgehog/intein (From Homology)	H: Hedgehog/intein	T: Hint	F: PF05203	ECOD (1.6)
B	PF05203	e6mx6B1	A: beta complex topology	X: Hedgehog/intein (From Homology)	H: Hedgehog/intein	T: Hint	F: PF05203	ECOD (1.6)
C	PF05203	e6mx6C1	A: beta complex topology	X: Hedgehog/intein (From Homology)	H: Hedgehog/intein	T: Hint	F: PF05203	ECOD (1.6)
D	PF05203	e6mx6D1	A: beta complex topology	X: Hedgehog/intein (From Homology)	H: Hedgehog/intein	T: Hint	F: PF05203	ECOD (1.6)
E	PF05203	e6mx6E1	A: beta complex topology	X: Hedgehog/intein (From Homology)	H: Hedgehog/intein	T: Hint	F: PF05203	ECOD (1.6)
F	PF05203	e6mx6F1	A: beta complex topology	X: Hedgehog/intein (From Homology)	H: Hedgehog/intein	T: Hint	F: PF05203	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F	PF10596	U6-snRNA interacting domain of PrP8 (U6-snRNA_bdg)	U6-snRNA interacting domain of PrP8	This domain incorporates the interacting site for the U6-snRNA as part of the U4/U6.U5 tri-snRNPs complex of the spliceosome, and is the prime candidate for the role of cofactor for the spliceosome's RNA core. The essential spliceosomal protein Prp8 ...	This domain incorporates the interacting site for the U6-snRNA as part of the U4/U6.U5 tri-snRNPs complex of the spliceosome, and is the prime candidate for the role of cofactor for the spliceosome's RNA core. The essential spliceosomal protein Prp8 interacts with U5 and U6 snRNAs and with specific pre-mRNA sequences that participate in catalysis. This close association with crucial RNA sequences, together with extensive genetic evidence, suggests that Prp8 could directly affect the function of the catalytic core, perhaps acting as a splicing cofactor [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F	Pre-mRNA-processing-splicing factor 8	peptidase activity catalytic activity, acting on a protein metallopeptidase activity hydrolase activity catalytic activity RNA binding U6 snRNA binding snRNA binding binding organic cyclic compound binding nucleic acid binding U5 snRNA binding	RNA splicing cellular biosynthetic process cellular metabolic process RNA metabolic process primary metabolic process gene expression mRNA processing mRNA metabolic process macromolecule biosynthetic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process biosynthetic process RNA splicing, via transesterification reactions RNA splicing cellular biosynthetic process cellular metabolic process RNA metabolic process primary metabolic process gene expression mRNA processing mRNA metabolic process macromolecule biosynthetic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process biosynthetic process RNA splicing, via transesterification reactions mRNA splicing, via spliceosome organic substance biosynthetic process metabolic process cellular process RNA splicing, via transesterification reactions with bulged adenosine as nucleophile macromolecule metabolic process RNA processing	membrane-bounded organelle protein-containing complex intracellular anatomical structure nucleus nuclear protein-containing complex intracellular membrane-bounded organelle cellular anatomical entity intracellular organelle spliceosomal complex organelle ribonucleoprotein complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F	IPR043173	Prp8 RNase domain IV, fingers region	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR036844	Hint domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR012984	PROCT domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR012591	PRO8NT domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR021983	PRP8 domain IV core	Domain
A, B, C, D, E A, B, C, D, E, F	IPR019580	Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding	Domain
A, B, C, D, E A, B, C, D, E, F	IPR019581	Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding	Domain
A, B, C, D, E A, B, C, D, E, F	IPR027652	Pre-mRNA-processing-splicing factor 8	Family
A, B, C, D, E A, B, C, D, E, F	IPR019582	RNA recognition motif, spliceosomal PrP8	Domain
A, B, C, D, E A, B, C, D, E, F	IPR043172	Prp8 RNase domain IV, palm region	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR012592	PROCN domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR042516	Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR012337	Ribonuclease H-like superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR000555	JAB1/MPN/MOV34 metalloenzyme domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.