6MHY

Structure of connexin-50 intercellular gap junction channel at 3.4 angstrom resolution by cryoEM

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD
Membrane Protein Annotation: mpstruc

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Connexin-like	8079721	4005615	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Claudin-like	8092545	3002606	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00029	e6mhyA1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
B	PF00029	e6mhyB1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
H	PF00029	e6mhyH1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
I	PF00029	e6mhyI1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
C	PF00029	e6mhyC1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
D	PF00029	e6mhyD1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
E	PF00029	e6mhyE1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
F	PF00029	e6mhyF1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
G	PF00029	e6mhyG1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
J	PF00029	e6mhyJ1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
K	PF00029	e6mhyK1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
L	PF00029	e6mhyL1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	PF00029	Connexin (Connexin)	Connexin	Connexin proteins form gap-junctions between cells. They carry four transmembrane regions, hence why this family now includes Connexin_CCC, which represented the second pair of TMs.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	Gap junction alpha-8 protein, connexin-50	wide pore channel activity passive transmembrane transporter activity channel activity transporter activity gap junction channel activity transmembrane transporter activity	gap junction-mediated intercellular transport localization intercellular transport transport cellular process establishment of localization regulation of biological process signaling biological regulation cell-cell signaling cell communication	cell-cell junction protein-containing complex anchoring junction plasma membrane protein complex gap junction membrane plasma membrane membrane protein complex cellular anatomical entity cell periphery cell junction connexin complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR038359	Connexin, N-terminal domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR002266	Gap junction alpha-8 protein (Cx50)	Family
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR019570	Gap junction protein, cysteine-rich domain	Domain
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR000500	Connexin	Family
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR017990	Connexin, conserved site	Conserved Site
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR013092	Connexin, N-terminal	Domain

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	OPM	Transmembrane	Alpha-helical polytopic	Tetraspan junctional complexes	Connexin

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	MemProtMD	Annotated as membrane protein by MemProtMD

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	Channels: Gap Junctions and Related Channels	connexin (Cx)-46/50 intercellular gap junction channels. Cx46 channel

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.