6KYT

The structure of the M. tb toxin MazEF-mt1 complex

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d6kyta_	All beta proteins	SH3-like barrel	Cell growth inhibitor/plasmid maintenance toxic component	automated matches	automated matches	(Mycobacterium tuberculosis H37Rv ) [TaxId: 83332 ],	SCOPe (2.08)
B	d6kytb1	All beta proteins	SH3-like barrel	Cell growth inhibitor/plasmid maintenance toxic component	automated matches	automated matches	(Mycobacterium tuberculosis H37Rv ) [TaxId: 83332 ],	SCOPe (2.08)
B	d6kytb2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Mycobacterium tuberculosis H37Rv ) [TaxId: 83332 ],	SCOPe (2.08)
D	d6kytd1	All beta proteins	SH3-like barrel	Cell growth inhibitor/plasmid maintenance toxic component	automated matches	automated matches	(Mycobacterium tuberculosis H37Rv ) [TaxId: 83332 ],	SCOPe (2.08)
D	d6kytd2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Mycobacterium tuberculosis H37Rv ) [TaxId: 83332 ],	SCOPe (2.08)
E	d6kyte_	All beta proteins	SH3-like barrel	Cell growth inhibitor/plasmid maintenance toxic component	automated matches	automated matches	(Mycobacterium tuberculosis H37Rv ) [TaxId: 83332 ],	SCOPe (2.08)
G	d6kytg1	All beta proteins	SH3-like barrel	Cell growth inhibitor/plasmid maintenance toxic component	automated matches	automated matches	(Mycobacterium tuberculosis H37Rv ) [TaxId: 83332 ],	SCOPe (2.08)
G	d6kytg2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Mycobacterium tuberculosis H37Rv ) [TaxId: 83332 ],	SCOPe (2.08)
H	d6kyth1	All beta proteins	SH3-like barrel	Cell growth inhibitor/plasmid maintenance toxic component	automated matches	automated matches	(Mycobacterium tuberculosis H37Rv ) [TaxId: 83332 ],	SCOPe (2.08)
H	d6kyth2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Mycobacterium tuberculosis H37Rv ) [TaxId: 83332 ],	SCOPe (2.08)
I [auth J]	d6kytj1	All beta proteins	SH3-like barrel	Cell growth inhibitor/plasmid maintenance toxic component	automated matches	automated matches	(Mycobacterium tuberculosis H37Rv ) [TaxId: 83332 ],	SCOPe (2.08)
I [auth J]	d6kytj2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Mycobacterium tuberculosis H37Rv ) [TaxId: 83332 ],	SCOPe (2.08)
J [auth K]	d6kytk1	All beta proteins	SH3-like barrel	Cell growth inhibitor/plasmid maintenance toxic component	automated matches	automated matches	(Mycobacterium tuberculosis H37Rv ) [TaxId: 83332 ],	SCOPe (2.08)
J [auth K]	d6kytk2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Mycobacterium tuberculosis H37Rv ) [TaxId: 83332 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Cell growth inhibitor/plasmid maintenance toxic component	8076061	3000352	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Cell growth inhibitor/plasmid maintenance toxic component	8076061	3000352	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Cell growth inhibitor/plasmid maintenance toxic component	8076061	3000352	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Cell growth inhibitor/plasmid maintenance toxic component	8076061	3000352	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Cell growth inhibitor/plasmid maintenance toxic component	8076061	3000352	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Cell growth inhibitor/plasmid maintenance toxic component	8076061	3000352	SCOP2B (2022-06-29)
I [auth J]	SCOP2B Superfamily	Cell growth inhibitor/plasmid maintenance toxic component	8076061	3000352	SCOP2B (2022-06-29)
J [auth K]	SCOP2B Superfamily	Cell growth inhibitor/plasmid maintenance toxic component	8076061	3000352	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PemK_toxin	e6kytA1	A: beta barrels	X: SH3	H: SH3	T: SH3	F: PemK_toxin	ECOD (1.6)
B	PemK_toxin	e6kytB1	A: beta barrels	X: SH3	H: SH3	T: SH3	F: PemK_toxin	ECOD (1.6)
D	PemK_toxin	e6kytD1	A: beta barrels	X: SH3	H: SH3	T: SH3	F: PemK_toxin	ECOD (1.6)
E	PemK_toxin	e6kytE1	A: beta barrels	X: SH3	H: SH3	T: SH3	F: PemK_toxin	ECOD (1.6)
G	PemK_toxin	e6kytG1	A: beta barrels	X: SH3	H: SH3	T: SH3	F: PemK_toxin	ECOD (1.6)
H	PemK_toxin	e6kytH1	A: beta barrels	X: SH3	H: SH3	T: SH3	F: PemK_toxin	ECOD (1.6)
I [auth J]	PemK_toxin	e6kytJ1	A: beta barrels	X: SH3	H: SH3	T: SH3	F: PemK_toxin	ECOD (1.6)
J [auth K]	PemK_toxin	e6kytK1	A: beta barrels	X: SH3	H: SH3	T: SH3	F: PemK_toxin	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, D, E, G, A, B, D, E, G, H, I [auth J], J [auth K]	PF02452	PemK-like, MazF-like toxin of type II toxin-antitoxin system (PemK_toxin)	PemK-like, MazF-like toxin of type II toxin-antitoxin system	PemK is a growth inhibitor in E. coli known to bind to the promoter region of the Pem operon, auto-regulating synthesis. This family represents the toxin molecule of a typical bacterial toxin-antitoxin system pairing. The family includes a number of ...	PemK is a growth inhibitor in E. coli known to bind to the promoter region of the Pem operon, auto-regulating synthesis. This family represents the toxin molecule of a typical bacterial toxin-antitoxin system pairing. The family includes a number of different toxins, such as MazF, Kid, PemK, ChpA, ChpB and ChpAK.	Domain
C [auth P], F, K [auth Q], L [auth C]	PF01402	Ribbon-helix-helix protein, copG family (RHH_1)	Ribbon-helix-helix protein, copG family	The structure of this protein repressor, which is the shortest reported to date and the first isolated from a plasmid, has a homodimeric ribbon-helix-helix arrangement [2]. The helix-turn-helix-like structure is involved in dimerisation and not DNA b ...	The structure of this protein repressor, which is the shortest reported to date and the first isolated from a plasmid, has a homodimeric ribbon-helix-helix arrangement [2]. The helix-turn-helix-like structure is involved in dimerisation and not DNA binding as might have been expected [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, D, E, G, A, B, D, E, G, H, I [auth J], J [auth K]	Endoribonuclease MazF9	DNA binding binding organic cyclic compound binding nucleic acid binding endonuclease activity nuclease activity hydrolase activity, acting on ester bonds catalytic activity, acting on RNA RNA endonuclease activity catalytic activity, acting on a nucleic acid RNA nuclease activity hydrolase activity catalytic activity	catabolic process RNA metabolic process primary metabolic process rRNA catabolic process nucleic acid metabolic process macromolecule catabolic process organic cyclic compound catabolic process nucleobase-containing compound catabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process ncRNA metabolic process nucleic acid catabolic process metabolic process catabolic process RNA metabolic process primary metabolic process rRNA catabolic process nucleic acid metabolic process macromolecule catabolic process organic cyclic compound catabolic process nucleobase-containing compound catabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process ncRNA metabolic process nucleic acid catabolic process metabolic process macromolecule metabolic process rRNA metabolic process organic substance catabolic process RNA catabolic process regulation of biological process regulation of growth negative regulation of growth negative regulation of biological process biological regulation growth regulation of cellular process cellular biosynthetic process negative regulation of cellular metabolic process organic substance biosynthetic process negative regulation of cellular process negative regulation of macromolecule metabolic process regulation of biosynthetic process mRNA catabolic process cellular metabolic process negative regulation of metabolic process regulation of cellular metabolic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process mRNA metabolic process negative regulation of cellular biosynthetic process regulation of cellular biosynthetic process regulation of gene expression negative regulation of gene expression biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process regulation of macromolecule biosynthetic process cellular process regulation of metabolic process biological process involved in interspecies interaction between organisms modulation by symbiont of host process modulation of process of another organism biological process involved in interaction with host biological process involved in symbiotic interaction	-
C [auth P], F, K [auth Q], L [auth C]	Antitoxin MazE9	molecular sequestering activity toxin sequestering activity	regulation of cellular process cellular biosynthetic process RNA metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process organic substance metabolic process organic substance biosynthetic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of cellular process cellular biosynthetic process RNA metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process organic substance metabolic process organic substance biosynthetic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of biological process cellular metabolic process biological regulation primary metabolic process regulation of cellular metabolic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process organic cyclic compound biosynthetic process metabolic process regulation of macromolecule biosynthetic process cellular process regulation of DNA-templated transcription macromolecule metabolic process regulation of metabolic process positive regulation of growth regulation of growth positive regulation of biological process growth response to stimulus response to chemical detoxification response to toxic substance	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, D, E, G, A, B, D, E, G, H, I [auth J], J [auth K]	IPR011067	Plasmid maintenance toxin/Cell growth inhibitor	Homologous Superfamily
A, B, D, E, G, A, B, D, E, G, H, I [auth J], J [auth K]	IPR003477	mRNA interferase PemK-like	Family
C [auth P], F, K [auth Q], L [auth C]	IPR002145	Ribbon-helix-helix protein, CopG	Domain

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.