Annotations: 6GWJ

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth B]	PF09341	e6gwjB1	A: a+b two layers	X: protein PCC1 (From Topology)	H: protein PCC1 (From Topology)	T: protein PCC1	F: PF09341	ECOD (1.6)
B [auth D]	PF15387	e6gwjD1	A: a+b two layers	X: EKC/KEOPS complex subunit GON7 (From Topology)	H: EKC/KEOPS complex subunit GON7 (From Topology)	T: EKC/KEOPS complex subunit GON7	F: PF15387	ECOD (1.6)
C [auth K]	PF00814	e6gwjK2	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: PF00814	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C [auth K]	3.30.420.40	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (utative)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth B]	PF09341	Transcription factor Pcc1 (Pcc1)	Transcription factor Pcc1	-	Family
B [auth D]	PF15387	Domain of unknown function (DUF4611) (DUF4611)	Domain of unknown function (DUF4611)	-	Family
C [auth K]	PF00814	tRNA N6-adenosine threonylcarbamoyltransferase (TsaD)	tRNA N6-adenosine threonylcarbamoyltransferase	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth B]	EKC/KEOPS complex subunit LAGE3	-	cellular biosynthetic process cellular metabolic process RNA metabolic process primary metabolic process gene expression macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process biosynthetic process tRNA processing organic substance biosynthetic process nucleic acid catabolic process cellular biosynthetic process cellular metabolic process RNA metabolic process primary metabolic process gene expression macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process biosynthetic process tRNA processing organic substance biosynthetic process nucleic acid catabolic process metabolic process cellular process macromolecule metabolic process RNA processing tRNA threonylcarbamoyladenosine metabolic process	protein-containing complex EKC/KEOPS complex intracellular anatomical structure cytoplasm cellular anatomical entity membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nuclear body intracellular organelle nucleoplasm organelle lumen protein-containing complex EKC/KEOPS complex intracellular anatomical structure cytoplasm cellular anatomical entity membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nuclear body intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen
B [auth D]	EKC/KEOPS complex subunit GON7	-	tRNA threonylcarbamoyladenosine modification cellular biosynthetic process RNA metabolic process nucleobase-containing compound metabolic process organic substance metabolic process tRNA processing organic substance biosynthetic process tRNA threonylcarbamoyladenosine metabolic process RNA processing cellular metabolic process tRNA modification primary metabolic process gene expression macromolecule biosynthetic process tRNA threonylcarbamoyladenosine modification cellular biosynthetic process RNA metabolic process nucleobase-containing compound metabolic process organic substance metabolic process tRNA processing organic substance biosynthetic process tRNA threonylcarbamoyladenosine metabolic process RNA processing cellular metabolic process tRNA modification primary metabolic process gene expression macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process organic cyclic compound metabolic process RNA modification biosynthetic process macromolecule modification nucleic acid catabolic process metabolic process cellular process macromolecule metabolic process	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle nucleolus intracellular organelle lumen intracellular organelle organelle lumen organelle intracellular non-membrane-bounded organelle intracellular anatomical structure cytoplasm cellular anatomical entity cytosol membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle nucleolus intracellular organelle lumen intracellular organelle organelle lumen organelle intracellular non-membrane-bounded organelle nuclear lumen non-membrane-bounded organelle protein-containing complex EKC/KEOPS complex nucleoplasm
C [auth K]	Probable tRNA N6-adenosine threonylcarbamoyltransferase	acyltransferase activity N(6)-L-threonylcarbamoyladenine synthase activity acyltransferase activity, transferring groups other than amino-acyl groups transferase activity catalytic activity cation binding ion binding binding metal ion binding small molecule binding	cellular biosynthetic process cellular metabolic process tRNA modification RNA metabolic process primary metabolic process gene expression macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process RNA modification organic substance metabolic process biosynthetic process tRNA processing cellular biosynthetic process cellular metabolic process tRNA modification RNA metabolic process primary metabolic process gene expression macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process RNA modification organic substance metabolic process biosynthetic process tRNA processing macromolecule modification organic substance biosynthetic process nucleic acid catabolic process metabolic process cellular process macromolecule metabolic process RNA processing tRNA threonylcarbamoyladenosine modification tRNA threonylcarbamoyladenosine metabolic process	protein-containing complex EKC/KEOPS complex intracellular anatomical structure cytoplasm cellular anatomical entity cytosol membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen protein-containing complex EKC/KEOPS complex intracellular anatomical structure cytoplasm cellular anatomical entity cytosol membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth B]	IPR015419	CTAG/Pcc1 family	Family
B [auth D]	IPR027893	EKC/KEOPS complex subunit GON7, metazoa	Family
C [auth K]	IPR017860	Peptidase M22, conserved site	Conserved Site
C [auth K]	IPR043129	ATPase, nucleotide binding domain	Homologous Superfamily
C [auth K]	IPR000905	Gcp-like domain	Domain
C [auth K]	IPR034680	tRNA N6-adenosine threonylcarbamoyltransferase Kae1, archaeal/eukaryotic	Family
C [auth K]	IPR017861	Kae1/TsaD family	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A [auth B]	Pharos: Q14657	Galloway-Mowat syndrome 2, X-linked Galloway-Mowat syndrome breast cancer epilepsy microcephaly breast carcinoma invasive ductal breast carcinoma ovarian cancer ductal breast carcinoma in situ Galloway-Mowat syndrome 1 malignant mesothelioma psoriasis
B [auth D]	Pharos: Q9BXV9	acute myeloid leukemia Galloway-Mowat syndrome osteosarcoma adenoma malignant ependymoma pancreatic ductal adenocarcinoma epilepsy nasopharyngeal carcinoma tuberculosis
C [auth K]	Pharos: Q9NPF4	Galloway-Mowat syndrome 3 Galloway-Mowat syndrome diabetes mellitus Galloway-Mowat syndrome 1 Malformations of Cortical Development osteosarcoma Pick disease Takayasu arteritis epilepsy medulloblastoma peripheral nervous system disease psoriasis intellectual disability microcephaly peripheral neuropathy Galloway-Mowat syndrome 3 Galloway-Mowat syndrome diabetes mellitus Galloway-Mowat syndrome 1 Malformations of Cortical Development osteosarcoma Pick disease Takayasu arteritis epilepsy medulloblastoma peripheral nervous system disease psoriasis intellectual disability microcephaly peripheral neuropathy renal tubular acidosis

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.