6FAH

Molecular basis of the flavin-based electron-bifurcating caffeyl-CoA reductase reaction

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B	d6fahb_	Alpha and beta proteins (a/b)	Adenine nucleotide alpha hydrolase-like	Adenine nucleotide alpha hydrolases-like	automated matches	automated matches	(Acetobacterium woodii DSM 1030 ) [TaxId: 931626 ],	SCOPe (2.08)
F	d6fahf_	Alpha and beta proteins (a/b)	Adenine nucleotide alpha hydrolase-like	Adenine nucleotide alpha hydrolases-like	automated matches	automated matches	(Acetobacterium woodii DSM 1030 ) [TaxId: 931626 ],	SCOPe (2.08)
D	d6fahd2	All alpha proteins	Bromodomain-like	Acyl-CoA dehydrogenase C-terminal domain-like	automated matches	automated matches	(Acetobacterium woodii DSM 1030 ) [TaxId: 931626 ],	SCOPe (2.08)
D	d6fahd1	Multi-domain proteins (alpha and beta)	Acyl-CoA dehydrogenase NM domain-like	Acyl-CoA dehydrogenase NM domain-like	automated matches	automated matches	(Acetobacterium woodii DSM 1030 ) [TaxId: 931626 ],	SCOPe (2.08)
C	d6fahc2	All alpha proteins	Bromodomain-like	Acyl-CoA dehydrogenase C-terminal domain-like	automated matches	automated matches	(Acetobacterium woodii DSM 1030 ) [TaxId: 931626 ],	SCOPe (2.08)
C	d6fahc1	Multi-domain proteins (alpha and beta)	Acyl-CoA dehydrogenase NM domain-like	Acyl-CoA dehydrogenase NM domain-like	automated matches	automated matches	(Acetobacterium woodii DSM 1030 ) [TaxId: 931626 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2 Family	ETFP subunits	8080635	4003848	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Adenine nucleotide alpha hydrolases-like	8080636	3001593	SCOP2 (2022-06-29)
F	SCOP2B Superfamily	Adenine nucleotide alpha hydrolases-like	8080636	3001593	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Fer4_21_1	e6fahA2	A: a+b two layers	X: 4Fe-4S ferredoxin (From Topology)	H: 4Fe-4S ferredoxin (From Topology)	T: 4Fe-4S ferredoxin	F: Fer4_21_1	ECOD (1.6)
A	ETF_alpha	e6fahA3	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: DHS-like NAD/FAD-binding domain	F: ETF_alpha	ECOD (1.6)
A	ETF	e6fahA1	A: a/b three-layered sandwiches	X: HUP domain-like	H: HUP domains (From Topology)	T: HUP domains	F: ETF	ECOD (1.6)
E	Fer4_21_1	e6fahE3	A: a+b two layers	X: 4Fe-4S ferredoxin (From Topology)	H: 4Fe-4S ferredoxin (From Topology)	T: 4Fe-4S ferredoxin	F: Fer4_21_1	ECOD (1.6)
E	ETF_alpha	e6fahE2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: DHS-like NAD/FAD-binding domain	F: ETF_alpha	ECOD (1.6)
E	ETF	e6fahE1	A: a/b three-layered sandwiches	X: HUP domain-like	H: HUP domains (From Topology)	T: HUP domains	F: ETF	ECOD (1.6)
B	ETF	e6fahB1	A: a/b three-layered sandwiches	X: HUP domain-like	H: HUP domains (From Topology)	T: HUP domains	F: ETF	ECOD (1.6)
F	ETF	e6fahF1	A: a/b three-layered sandwiches	X: HUP domain-like	H: HUP domains (From Topology)	T: HUP domains	F: ETF	ECOD (1.6)
D	Acyl-CoA_dh_M	e6fahD2	A: beta complex topology	X: Acyl-CoA dehydrogenase middle domain-like (From Topology)	H: Acyl-CoA dehydrogenase middle domain-like (From Topology)	T: Acyl-CoA dehydrogenase middle domain-like	F: Acyl-CoA_dh_M	ECOD (1.6)
D	Acyl-CoA_dh_N	e6fahD3	A: alpha arrays	X: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	H: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase N-terminal domain-like	F: Acyl-CoA_dh_N	ECOD (1.6)
D	Acyl-CoA_dh_1	e6fahD1	A: alpha bundles	X: Bromodomain-like	H: Acyl-CoA dehydrogenase C-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase C-terminal domain-like	F: Acyl-CoA_dh_1	ECOD (1.6)
C	Acyl-CoA_dh_M	e6fahC3	A: beta complex topology	X: Acyl-CoA dehydrogenase middle domain-like (From Topology)	H: Acyl-CoA dehydrogenase middle domain-like (From Topology)	T: Acyl-CoA dehydrogenase middle domain-like	F: Acyl-CoA_dh_M	ECOD (1.6)
C	Acyl-CoA_dh_N	e6fahC2	A: alpha arrays	X: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	H: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase N-terminal domain-like	F: Acyl-CoA_dh_N	ECOD (1.6)
C	Acyl-CoA_dh_1	e6fahC1	A: alpha bundles	X: Bromodomain-like	H: Acyl-CoA dehydrogenase C-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase C-terminal domain-like	F: Acyl-CoA_dh_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	3.40.50.620	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	HUPs	CATH (4.3.0)
F	3.40.50.620	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	HUPs	CATH (4.3.0)
D	1.10.540.10	Mainly Alpha	Orthogonal Bundle	Butyryl-Coa Dehydrogenase, subunit A	domain 1	CATH (4.3.0)
D	2.40.110.10	Mainly Beta	Beta Barrel	Butyryl-CoA Dehydrogenase, subunit A	domain 2	CATH (4.3.0)
D	1.20.140.10	Mainly Alpha	Up-down Bundle	Butyryl-CoA Dehydrogenase, subunit A	domain 3	CATH (4.3.0)
C	1.10.540.10	Mainly Alpha	Orthogonal Bundle	Butyryl-Coa Dehydrogenase, subunit A	domain 1	CATH (4.3.0)
C	2.40.110.10	Mainly Beta	Beta Barrel	Butyryl-CoA Dehydrogenase, subunit A	domain 2	CATH (4.3.0)
C	1.20.140.10	Mainly Alpha	Up-down Bundle	Butyryl-CoA Dehydrogenase, subunit A	domain 3	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, E	PF00766	Electron transfer flavoprotein FAD-binding domain (ETF_alpha)	Electron transfer flavoprotein FAD-binding domain	This domain found at the C-terminus of electron transfer flavoprotein alpha chain and binds to FAD [1]. The fold consists of a five-stranded parallel beta sheet as the core of the domain, flanked by alternating helices. A small part of this domain i ...	This domain found at the C-terminus of electron transfer flavoprotein alpha chain and binds to FAD [1]. The fold consists of a five-stranded parallel beta sheet as the core of the domain, flanked by alternating helices. A small part of this domain is donated by the beta chain [1].	Domain
A, E	PF14697	4Fe-4S dicluster domain (Fer4_21)	4Fe-4S dicluster domain	Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contai ...	Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contains two 4Fe4S clusters.	Domain
A, E	PF01012	Electron transfer flavoprotein domain (ETF)	Electron transfer flavoprotein domain	This family includes the homologous domain shared between the alpha and beta subunits of the electron transfer flavoprotein [1].	Domain
B, F	PF01012	Electron transfer flavoprotein domain (ETF)	Electron transfer flavoprotein domain	This family includes the homologous domain shared between the alpha and beta subunits of the electron transfer flavoprotein [1].	Domain
C, D	PF02771	Acyl-CoA dehydrogenase, N-terminal domain (Acyl-CoA_dh_N)	Acyl-CoA dehydrogenase, N-terminal domain	The N-terminal domain of Acyl-CoA dehydrogenase is an all-alpha domain.	Domain
C, D	PF02770	Acyl-CoA dehydrogenase, middle domain (Acyl-CoA_dh_M)	Acyl-CoA dehydrogenase, middle domain	Central domain of Acyl-CoA dehydrogenase has a beta-barrel fold.	Domain
C, D	PF00441	Acyl-CoA dehydrogenase, C-terminal domain (Acyl-CoA_dh_1)	Acyl-CoA dehydrogenase, C-terminal domain	C-terminal domain of Acyl-CoA dehydrogenase is an all-alpha, four helical up-and-down bundle.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, E	Caffeyl-CoA reductase-Etf complex subunit CarE	FAD binding flavin adenine dinucleotide binding binding nucleoside phosphate binding ion binding organic cyclic compound binding anion binding nucleotide binding heterocyclic compound binding small molecule binding oxidoreductase activity oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on the CH-CH group of donors catalytic activity FAD binding flavin adenine dinucleotide binding binding nucleoside phosphate binding ion binding organic cyclic compound binding anion binding nucleotide binding heterocyclic compound binding small molecule binding oxidoreductase activity oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on the CH-CH group of donors catalytic activity iron-sulfur cluster binding 4 iron, 4 sulfur cluster binding metal cluster binding electron transfer activity cation binding metal ion binding	-	intracellular anatomical structure cytoplasm cellular anatomical entity
B, F	Caffeyl-CoA reductase-Etf complex subunit CarD	oxidoreductase activity oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on the CH-CH group of donors catalytic activity FAD binding flavin adenine dinucleotide binding binding nucleoside phosphate binding ion binding organic cyclic compound binding anion binding nucleotide binding heterocyclic compound binding small molecule binding oxidoreductase activity oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on the CH-CH group of donors catalytic activity FAD binding flavin adenine dinucleotide binding binding nucleoside phosphate binding ion binding organic cyclic compound binding anion binding nucleotide binding heterocyclic compound binding small molecule binding electron transfer activity	-	intracellular anatomical structure cytoplasm cellular anatomical entity
C, D	Caffeyl-CoA reductase-Etf complex subunit CarC	oxidoreductase activity oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on the CH-CH group of donors catalytic activity FAD binding flavin adenine dinucleotide binding binding nucleoside phosphate binding ion binding organic cyclic compound binding anion binding nucleotide binding heterocyclic compound binding small molecule binding oxidoreductase activity oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on the CH-CH group of donors catalytic activity FAD binding flavin adenine dinucleotide binding binding nucleoside phosphate binding ion binding organic cyclic compound binding anion binding nucleotide binding heterocyclic compound binding small molecule binding oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor acyl-CoA dehydrogenase activity	-	intracellular anatomical structure cytoplasm cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, E	IPR014730	Electron transfer flavoprotein, alpha/beta-subunit, N-terminal	Domain
A, E	IPR001308	Electron transfer flavoprotein alpha subunit/FixB	Family
A, E	IPR014731	Electron transfer flavoprotein, alpha subunit, C-terminal	Domain
A, E	IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	Homologous Superfamily
A, E	IPR017900	4Fe-4S ferredoxin, iron-sulphur binding, conserved site	Conserved Site
A, E	IPR017896	4Fe-4S ferredoxin-type, iron-sulphur binding domain	Domain
A, E	IPR029035	DHS-like NAD/FAD-binding domain superfamily	Homologous Superfamily
A, E	IPR033947	Electron transfer flavoprotein, alpha subunit, N-terminal	Domain
B, F	IPR014730	Electron transfer flavoprotein, alpha/beta-subunit, N-terminal	Domain
B, F	IPR033948	Electron transfer flavoprotein, beta subunit, N-terminal	Domain
B, F	IPR000049	Electron transfer flavoprotein, beta-subunit, conserved site	Conserved Site
B, F	IPR012255	Electron transfer flavoprotein, beta subunit	Family
B, F	IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	Homologous Superfamily
C, D	IPR009100	Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain superfamily	Homologous Superfamily
C, D	IPR046373	Acyl-CoA oxidase/dehydrogenase, middle domain superfamily	Homologous Superfamily
C, D	IPR006091	Acyl-CoA oxidase/dehydrogenase, middle domain	Domain
C, D	IPR009075	Acyl-CoA dehydrogenase/oxidase, C-terminal	Domain
C, D	IPR006089	Acyl-CoA dehydrogenase, conserved site	Conserved Site
C, D	IPR036250	Acyl-CoA dehydrogenase-like, C-terminal	Homologous Superfamily
C, D	IPR013786	Acyl-CoA dehydrogenase/oxidase, N-terminal	Domain
C, D	IPR037069	Acyl-CoA dehydrogenase/oxidase, N-terminal domain superfamily	Homologous Superfamily