6C9K

Single-Particle reconstruction of DARP14 - A designed protein scaffold displaying ~17kDa DARPin proteins

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
E	SCOP2B Superfamily	Tautomerase/MIF	8084399	3001770	SCOP2B (2022-06-29)
M	SCOP2B Superfamily	Tautomerase/MIF	8084399	3001770	SCOP2B (2022-06-29)
O	SCOP2B Superfamily	Tautomerase/MIF	8084399	3001770	SCOP2B (2022-06-29)
P	SCOP2B Superfamily	Tautomerase/MIF	8084399	3001770	SCOP2B (2022-06-29)
U	SCOP2B Superfamily	Tautomerase/MIF	8084399	3001770	SCOP2B (2022-06-29)
V	SCOP2B Superfamily	Tautomerase/MIF	8084399	3001770	SCOP2B (2022-06-29)
X	SCOP2B Superfamily	Tautomerase/MIF	8084399	3001770	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Tautomerase/MIF	8084399	3001770	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Tautomerase/MIF	8084399	3001770	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Tautomerase/MIF	8084399	3001770	SCOP2B (2022-06-29)
N	SCOP2B Superfamily	Tautomerase/MIF	8084399	3001770	SCOP2B (2022-06-29)
W	SCOP2B Superfamily	Tautomerase/MIF	8084399	3001770	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF01923	e6c9kA2	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Cobalamin adenosyltransferase (From Topology)	T: Cobalamin adenosyltransferase	F: PF01923	ECOD (1.6)
A	PF13857	e6c9kA1	A: alpha superhelices	X: Repetitive alpha hairpins	H: Ankyrin repeat (From Topology)	T: Ankyrin repeat	F: PF13857	ECOD (1.6)
C	PF01923	e6c9kC2	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Cobalamin adenosyltransferase (From Topology)	T: Cobalamin adenosyltransferase	F: PF01923	ECOD (1.6)
C	PF13857	e6c9kC1	A: alpha superhelices	X: Repetitive alpha hairpins	H: Ankyrin repeat (From Topology)	T: Ankyrin repeat	F: PF13857	ECOD (1.6)
J	PF01923	e6c9kJ2	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Cobalamin adenosyltransferase (From Topology)	T: Cobalamin adenosyltransferase	F: PF01923	ECOD (1.6)
J	PF13857	e6c9kJ1	A: alpha superhelices	X: Repetitive alpha hairpins	H: Ankyrin repeat (From Topology)	T: Ankyrin repeat	F: PF13857	ECOD (1.6)
L	PF01923	e6c9kL2	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Cobalamin adenosyltransferase (From Topology)	T: Cobalamin adenosyltransferase	F: PF01923	ECOD (1.6)
L	PF13857	e6c9kL1	A: alpha superhelices	X: Repetitive alpha hairpins	H: Ankyrin repeat (From Topology)	T: Ankyrin repeat	F: PF13857	ECOD (1.6)
Q	PF01923	e6c9kQ2	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Cobalamin adenosyltransferase (From Topology)	T: Cobalamin adenosyltransferase	F: PF01923	ECOD (1.6)
Q	PF13857	e6c9kQ1	A: alpha superhelices	X: Repetitive alpha hairpins	H: Ankyrin repeat (From Topology)	T: Ankyrin repeat	F: PF13857	ECOD (1.6)
R	PF01923	e6c9kR1	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Cobalamin adenosyltransferase (From Topology)	T: Cobalamin adenosyltransferase	F: PF01923	ECOD (1.6)
R	PF13857	e6c9kR2	A: alpha superhelices	X: Repetitive alpha hairpins	H: Ankyrin repeat (From Topology)	T: Ankyrin repeat	F: PF13857	ECOD (1.6)
B	PF01923	e6c9kB2	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Cobalamin adenosyltransferase (From Topology)	T: Cobalamin adenosyltransferase	F: PF01923	ECOD (1.6)
B	PF13857	e6c9kB1	A: alpha superhelices	X: Repetitive alpha hairpins	H: Ankyrin repeat (From Topology)	T: Ankyrin repeat	F: PF13857	ECOD (1.6)
D	PF01923	e6c9kD2	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Cobalamin adenosyltransferase (From Topology)	T: Cobalamin adenosyltransferase	F: PF01923	ECOD (1.6)
D	PF13857	e6c9kD1	A: alpha superhelices	X: Repetitive alpha hairpins	H: Ankyrin repeat (From Topology)	T: Ankyrin repeat	F: PF13857	ECOD (1.6)
I	PF01923	e6c9kI1	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Cobalamin adenosyltransferase (From Topology)	T: Cobalamin adenosyltransferase	F: PF01923	ECOD (1.6)
I	PF13857	e6c9kI2	A: alpha superhelices	X: Repetitive alpha hairpins	H: Ankyrin repeat (From Topology)	T: Ankyrin repeat	F: PF13857	ECOD (1.6)
K	PF01923	e6c9kK2	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Cobalamin adenosyltransferase (From Topology)	T: Cobalamin adenosyltransferase	F: PF01923	ECOD (1.6)
K	PF13857	e6c9kK1	A: alpha superhelices	X: Repetitive alpha hairpins	H: Ankyrin repeat (From Topology)	T: Ankyrin repeat	F: PF13857	ECOD (1.6)
S	PF01923	e6c9kS1	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Cobalamin adenosyltransferase (From Topology)	T: Cobalamin adenosyltransferase	F: PF01923	ECOD (1.6)
S	PF13857	e6c9kS2	A: alpha superhelices	X: Repetitive alpha hairpins	H: Ankyrin repeat (From Topology)	T: Ankyrin repeat	F: PF13857	ECOD (1.6)
T	PF01923	e6c9kT1	A: alpha bundles	X: Ferritin/Heme oxygenase/4-helical cytokines	H: Cobalamin adenosyltransferase (From Topology)	T: Cobalamin adenosyltransferase	F: PF01923	ECOD (1.6)
T	PF13857	e6c9kT2	A: alpha superhelices	X: Repetitive alpha hairpins	H: Ankyrin repeat (From Topology)	T: Ankyrin repeat	F: PF13857	ECOD (1.6)
E	PF02962	e6c9kE1	A: a+b two layers	X: Tautomerase/MIF-like	H: Tautomerase/MIF (From Topology)	T: Tautomerase/MIF	F: PF02962	ECOD (1.6)
M	PF02962	e6c9kM1	A: a+b two layers	X: Tautomerase/MIF-like	H: Tautomerase/MIF (From Topology)	T: Tautomerase/MIF	F: PF02962	ECOD (1.6)
O	PF02962	e6c9kO1	A: a+b two layers	X: Tautomerase/MIF-like	H: Tautomerase/MIF (From Topology)	T: Tautomerase/MIF	F: PF02962	ECOD (1.6)
P	PF02962	e6c9kP1	A: a+b two layers	X: Tautomerase/MIF-like	H: Tautomerase/MIF (From Topology)	T: Tautomerase/MIF	F: PF02962	ECOD (1.6)
U	PF02962	e6c9kU1	A: a+b two layers	X: Tautomerase/MIF-like	H: Tautomerase/MIF (From Topology)	T: Tautomerase/MIF	F: PF02962	ECOD (1.6)
V	PF02962	e6c9kV1	A: a+b two layers	X: Tautomerase/MIF-like	H: Tautomerase/MIF (From Topology)	T: Tautomerase/MIF	F: PF02962	ECOD (1.6)
X	PF02962	e6c9kX1	A: a+b two layers	X: Tautomerase/MIF-like	H: Tautomerase/MIF (From Topology)	T: Tautomerase/MIF	F: PF02962	ECOD (1.6)
F	PF02962	e6c9kF1	A: a+b two layers	X: Tautomerase/MIF-like	H: Tautomerase/MIF (From Topology)	T: Tautomerase/MIF	F: PF02962	ECOD (1.6)
G	PF02962	e6c9kG1	A: a+b two layers	X: Tautomerase/MIF-like	H: Tautomerase/MIF (From Topology)	T: Tautomerase/MIF	F: PF02962	ECOD (1.6)
H	PF02962	e6c9kH1	A: a+b two layers	X: Tautomerase/MIF-like	H: Tautomerase/MIF (From Topology)	T: Tautomerase/MIF	F: PF02962	ECOD (1.6)
N	PF02962	e6c9kN1	A: a+b two layers	X: Tautomerase/MIF-like	H: Tautomerase/MIF (From Topology)	T: Tautomerase/MIF	F: PF02962	ECOD (1.6)
W	PF02962	e6c9kW1	A: a+b two layers	X: Tautomerase/MIF-like	H: Tautomerase/MIF (From Topology)	T: Tautomerase/MIF	F: PF02962	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
E	3.30.429.10	Alpha Beta	2-Layer Sandwich	Macrophage Migration Inhibitory Factor	Macrophage Migration Inhibitory Factor	CATH (4.3.0)
M	3.30.429.10	Alpha Beta	2-Layer Sandwich	Macrophage Migration Inhibitory Factor	Macrophage Migration Inhibitory Factor	CATH (4.3.0)
O	3.30.429.10	Alpha Beta	2-Layer Sandwich	Macrophage Migration Inhibitory Factor	Macrophage Migration Inhibitory Factor	CATH (4.3.0)
P	3.30.429.10	Alpha Beta	2-Layer Sandwich	Macrophage Migration Inhibitory Factor	Macrophage Migration Inhibitory Factor	CATH (4.3.0)
U	3.30.429.10	Alpha Beta	2-Layer Sandwich	Macrophage Migration Inhibitory Factor	Macrophage Migration Inhibitory Factor	CATH (4.3.0)
V	3.30.429.10	Alpha Beta	2-Layer Sandwich	Macrophage Migration Inhibitory Factor	Macrophage Migration Inhibitory Factor	CATH (4.3.0)
X	3.30.429.10	Alpha Beta	2-Layer Sandwich	Macrophage Migration Inhibitory Factor	Macrophage Migration Inhibitory Factor	CATH (4.3.0)
F	3.30.429.10	Alpha Beta	2-Layer Sandwich	Macrophage Migration Inhibitory Factor	Macrophage Migration Inhibitory Factor	CATH (4.3.0)
G	3.30.429.10	Alpha Beta	2-Layer Sandwich	Macrophage Migration Inhibitory Factor	Macrophage Migration Inhibitory Factor	CATH (4.3.0)
H	3.30.429.10	Alpha Beta	2-Layer Sandwich	Macrophage Migration Inhibitory Factor	Macrophage Migration Inhibitory Factor	CATH (4.3.0)
N	3.30.429.10	Alpha Beta	2-Layer Sandwich	Macrophage Migration Inhibitory Factor	Macrophage Migration Inhibitory Factor	CATH (4.3.0)
W	3.30.429.10	Alpha Beta	2-Layer Sandwich	Macrophage Migration Inhibitory Factor	Macrophage Migration Inhibitory Factor	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
E, F, G, H, M E, F, G, H, M, N, O, P, U, V, W, X	PF02962	5-carboxymethyl-2-hydroxymuconate isomerase (CHMI)	5-carboxymethyl-2-hydroxymuconate isomerase	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, I A, B, C, D, I, J, K, L, Q, R, S, T	DARP14 - Subunit A with DARPin	-	-	-
E, F, G, H, M E, F, G, H, M, N, O, P, U, V, W, X	DARP14 - Subunit B	isomerase activity intramolecular oxidoreductase activity, transposing C=C bonds intramolecular oxidoreductase activity 5-carboxymethyl-2-hydroxymuconate delta-isomerase activity catalytic activity	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
E, F, G, H, M E, F, G, H, M, N, O, P, U, V, W, X	IPR014347	Tautomerase/MIF superfamily	Homologous Superfamily
E, F, G, H, M E, F, G, H, M, N, O, P, U, V, W, X	IPR004220	5-carboxymethyl-2-hydroxymuconate isomerase	Family