6AHC

Cryo-EM structure of aldehyde-alcohol dehydrogenase reveals a high-order helical architecture critical for its activity

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00465	e6ahcA1	A: alpha bundles	X: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	H: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	T: alpha-helical domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)
A	PF00465	e6ahcA2	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Rossmann-like domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)
B	PF00465	e6ahcB3	A: alpha bundles	X: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	H: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	T: alpha-helical domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)
B	PF00465	e6ahcB4	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Rossmann-like domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)
B	PF00171	e6ahcB2	A: a/b three-layered sandwiches	X: ALDH-like (From Topology)	H: ALDH-like (From Topology)	T: ALDH-like	F: PF00171	ECOD (1.6)
C	PF00465	e6ahcC3	A: alpha bundles	X: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	H: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	T: alpha-helical domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)
C	PF00465	e6ahcC4	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Rossmann-like domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)
C	PF00171	e6ahcC2	A: a/b three-layered sandwiches	X: ALDH-like (From Topology)	H: ALDH-like (From Topology)	T: ALDH-like	F: PF00171	ECOD (1.6)
D	PF00465	e6ahcD3	A: alpha bundles	X: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	H: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	T: alpha-helical domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)
D	PF00465	e6ahcD4	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Rossmann-like domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)
D	PF00171	e6ahcD2	A: a/b three-layered sandwiches	X: ALDH-like (From Topology)	H: ALDH-like (From Topology)	T: ALDH-like	F: PF00171	ECOD (1.6)
E	PF00465	e6ahcE3	A: alpha bundles	X: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	H: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	T: alpha-helical domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)
E	PF00465	e6ahcE4	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Rossmann-like domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)
E	PF00171	e6ahcE2	A: a/b three-layered sandwiches	X: ALDH-like (From Topology)	H: ALDH-like (From Topology)	T: ALDH-like	F: PF00171	ECOD (1.6)
F	PF00465	e6ahcF3	A: alpha bundles	X: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	H: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	T: alpha-helical domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)
F	PF00465	e6ahcF4	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Rossmann-like domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)
F	PF00171	e6ahcF2	A: a/b three-layered sandwiches	X: ALDH-like (From Topology)	H: ALDH-like (From Topology)	T: ALDH-like	F: PF00171	ECOD (1.6)
G	PF00465	e6ahcG3	A: alpha bundles	X: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	H: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	T: alpha-helical domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)
G	PF00465	e6ahcG4	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Rossmann-like domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)
G	PF00171	e6ahcG2	A: a/b three-layered sandwiches	X: ALDH-like (From Topology)	H: ALDH-like (From Topology)	T: ALDH-like	F: PF00171	ECOD (1.6)
H	PF00465	e6ahcH2	A: alpha bundles	X: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	H: alpha-helical domain in dehydroquinate synthase-like enzymes (From Topology)	T: alpha-helical domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)
H	PF00465	e6ahcH1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Rossmann-like domain in dehydroquinate synthase-like enzymes	F: PF00465	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.1970	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (utative)
A	1.20.1090.10	Mainly Alpha	Up-down Bundle	Dehydroquinate synthase-like, alpha domain	Dehydroquinate synthase-like - alpha domain	CATH (utative)
H	3.40.50.1970	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (utative)
H	1.20.1090.10	Mainly Alpha	Up-down Bundle	Dehydroquinate synthase-like, alpha domain	Dehydroquinate synthase-like - alpha domain	CATH (utative)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G, H	PF00171	Aldehyde dehydrogenase family (Aldedh)	Aldehyde dehydrogenase family	-	Family
A, B, C, D, E A, B, C, D, E, F, G, H	PF00465	Iron-containing alcohol dehydrogenase (Fe-ADH)	Iron-containing alcohol dehydrogenase	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G, H	Aldehyde-alcohol dehydrogenase	cation binding transition metal ion binding iron ion binding ion binding binding ferrous iron binding metal ion binding small molecule binding oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors catalytic activity acetaldehyde dehydrogenase (acetylating) activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor alcohol dehydrogenase [NAD(P)+] activity cation binding transition metal ion binding iron ion binding ion binding binding ferrous iron binding metal ion binding small molecule binding oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors catalytic activity acetaldehyde dehydrogenase (acetylating) activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor alcohol dehydrogenase [NAD(P)+] activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on CH-OH group of donors alcohol dehydrogenase (NAD+) activity, iron-dependent alcohol dehydrogenase (NAD+) activity identical protein binding protein binding	monosaccharide metabolic process mixed acid fermentation 2-oxoglutarate metabolic process oxoacid metabolic process energy derivation by oxidation of organic compounds organic hydroxy compound metabolic process alcohol metabolic process organic acid metabolic process fermentation lactate metabolic process succinate metabolic process glycolytic fermentation organic substance metabolic process small molecule metabolic process monosaccharide metabolic process mixed acid fermentation 2-oxoglutarate metabolic process oxoacid metabolic process energy derivation by oxidation of organic compounds organic hydroxy compound metabolic process alcohol metabolic process organic acid metabolic process fermentation lactate metabolic process succinate metabolic process glycolytic fermentation organic substance metabolic process small molecule metabolic process carbohydrate metabolic process cellular metabolic process dicarboxylic acid metabolic process carboxylic acid metabolic process acetate metabolic process hexose metabolic process primary metabolic process generation of precursor metabolites and energy ethanol metabolic process metabolic process cellular process monocarboxylic acid metabolic process formate metabolic process primary alcohol metabolic process glucose metabolic process small molecule biosynthetic process ethanol biosynthetic process biosynthetic process organic hydroxy compound biosynthetic process alcohol biosynthetic process organic substance biosynthetic process primary alcohol biosynthetic process carbon utilization response to nutrient levels response to stimulus response to oxidative stress response to stress cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein complex oligomerization protein-containing complex assembly protein homooligomerization cellular component organization or biogenesis	membrane cellular anatomical entity intracellular anatomical structure cytoplasm cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G, H	IPR015590	Aldehyde dehydrogenase domain	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	IPR016161	Aldehyde/histidinol dehydrogenase	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR016162	Aldehyde dehydrogenase, N-terminal	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR034789	Bifunctional aldehyde-alcohol dehydrogenase, C-terminal domain	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	IPR001670	Alcohol dehydrogenase, iron-type/glycerol dehydrogenase GldA	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	IPR012079	Bifunctional aldehyde-alcohol dehydrogenase	Family
A, B, C, D, E A, B, C, D, E, F, G, H	IPR016163	Aldehyde dehydrogenase, C-terminal	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR039697	Iron-type alcohol dehydrogenase-like	Family
A, B, C, D, E A, B, C, D, E, F, G, H	IPR018211	Alcohol dehydrogenase, iron-type, conserved site	Conserved Site

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6AHC

Cryo-EM structure of aldehyde-alcohol dehydrogenase reveals a high-order helical architecture critical for its activity

External Resource: Annotation

Domain Annotation: ECOD Classification ECOD Database Homepage

Domain Annotation: CATH CATH Database Homepage

Protein Family Annotation Pfam Database Homepage

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

InterPro: Protein Family Classification InterPro Database Homepage