5YQG

The structure of 14-3-3 and pNumb peptide

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Protein Modification Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d5yqga1	All alpha proteins	alpha-alpha superhelix	14-3-3 protein	14-3-3 protein	automated matches	Mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
A	d5yqga2	Artifacts	Tags	Tags	Tags	N-terminal Tags	Mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
B	d5yqgb_	All alpha proteins	alpha-alpha superhelix	14-3-3 protein	14-3-3 protein	automated matches	Mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
C	d5yqgc1	All alpha proteins	alpha-alpha superhelix	14-3-3 protein	14-3-3 protein	automated matches	Mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
C	d5yqgc2	Artifacts	Tags	Tags	Tags	N-terminal Tags	Mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
D	d5yqgd_	All alpha proteins	alpha-alpha superhelix	14-3-3 protein	14-3-3 protein	automated matches	Mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	14-3-3 protein	8082817	3001329	SCOP2B (2022-06-29)
B	SCOP2 Family	14-3-3 protein	8082816	4001338	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	14-3-3 protein	8082817	3001329	SCOP2 (2022-06-29)
C	SCOP2B Superfamily	14-3-3 protein	8082817	3001329	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	14-3-3 protein	8082817	3001329	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00244	e5yqgA1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: PF00244	ECOD (1.6)
B	PF00244	e5yqgB1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: PF00244	ECOD (1.6)
C	PF00244	e5yqgC1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: PF00244	ECOD (1.6)
D	PF00244	e5yqgD1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: PF00244	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.20.190.20	Mainly Alpha	Up-down Bundle	Delta-Endotoxin	domain 1	CATH (4.3.0)
B	1.20.190.20	Mainly Alpha	Up-down Bundle	Delta-Endotoxin	domain 1	CATH (4.3.0)
C	1.20.190.20	Mainly Alpha	Up-down Bundle	Delta-Endotoxin	domain 1	CATH (4.3.0)
D	1.20.190.20	Mainly Alpha	Up-down Bundle	Delta-Endotoxin	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF00244	14-3-3 protein (14-3-3)	14-3-3 protein	-	Repeat
E, F	PF06311	NUMB domain (NumbF)	NUMB domain	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	14-3-3 protein eta	transcription factor binding RNA polymerase II-specific DNA-binding transcription factor binding nuclear receptor binding protein binding binding nuclear glucocorticoid receptor binding DNA-binding transcription factor binding protein domain specific binding protein heterodimerization activity protein dimerization activity transmembrane transporter binding channel regulator activity ion channel regulator activity molecular function regulator activity transcription factor binding RNA polymerase II-specific DNA-binding transcription factor binding nuclear receptor binding protein binding binding nuclear glucocorticoid receptor binding DNA-binding transcription factor binding protein domain specific binding protein heterodimerization activity protein dimerization activity transmembrane transporter binding channel regulator activity ion channel regulator activity molecular function regulator activity sodium channel regulator activity transporter regulator activity cytoskeletal protein binding actin binding enzyme binding identical protein binding	trans-synaptic signaling regulation of biological process regulation of signaling regulation of cellular process modulation of chemical synaptic transmission signaling biological regulation cell-cell signaling presynaptic modulation of chemical synaptic transmission chemical synaptic transmission cell communication synaptic signaling regulation of trans-synaptic signaling anterograde trans-synaptic signaling trans-synaptic signaling regulation of biological process regulation of signaling regulation of cellular process modulation of chemical synaptic transmission signaling biological regulation cell-cell signaling presynaptic modulation of chemical synaptic transmission chemical synaptic transmission cell communication synaptic signaling regulation of trans-synaptic signaling anterograde trans-synaptic signaling regulation of cell communication cellular process regulation of localization transport monoatomic ion transmembrane transport regulation of transmembrane transporter activity sodium ion transmembrane transport regulation of monoatomic ion transmembrane transport regulation of sodium ion transport regulation of sodium ion transmembrane transport metal ion transport regulation of transporter activity monoatomic cation transmembrane transport regulation of sodium ion transmembrane transporter activity localization monoatomic ion transport monoatomic cation transport regulation of metal ion transport regulation of monoatomic ion transmembrane transporter activity regulation of transport regulation of monoatomic ion transport inorganic ion transmembrane transport establishment of localization inorganic cation transmembrane transport transmembrane transport regulation of molecular function regulation of transmembrane transport regulation of monoatomic cation transmembrane transport sodium ion transport catabolic process lipid catabolic process glucocorticoid catabolic process steroid metabolic process primary metabolic process organic cyclic compound catabolic process glucocorticoid metabolic process organic cyclic compound metabolic process steroid catabolic process organic substance metabolic process metabolic process organic substance catabolic process lipid metabolic process negative regulation of programmed cell death regulation of apoptotic process negative regulation of biological process regulation of programmed cell death cell death negative regulation of apoptotic process negative regulation of cellular process apoptotic process programmed cell death membrane depolarization during action potential membrane depolarization action potential regulation of biological quality regulation of membrane potential multicellular organismal process cell projection organization regulation of cell differentiation regulation of neurogenesis neuron projection development regulation of cell development multicellular organism development cell projection morphogenesis neuron differentiation neuron projection morphogenesis cell differentiation regulation of dendrite morphogenesis anatomical structure development system development developmental process negative regulation of cellular component organization regulation of cell projection organization nervous system development generation of neurons regulation of dendrite development negative regulation of multicellular organismal process regulation of neuron projection development regulation of cellular component organization regulation of developmental process cellular developmental process plasma membrane bounded cell projection morphogenesis cell development cell morphogenesis regulation of nervous system development cellular component organization negative regulation of neurogenesis neurogenesis dendrite development negative regulation of nervous system development regulation of multicellular organismal process negative regulation of cell differentiation cellular component organization or biogenesis negative regulation of cell projection organization regulation of anatomical structure morphogenesis regulation of multicellular organismal development plasma membrane bounded cell projection organization negative regulation of dendrite morphogenesis negative regulation of developmental process dendrite morphogenesis cell morphogenesis involved in neuron differentiation negative regulation of cell development anatomical structure morphogenesis neuron development regulation of plasma membrane bounded cell projection organization cellular response to stimulus response to stimulus signal transduction response to organic substance intracellular receptor signaling pathway response to lipid hormone-mediated signaling pathway cellular response to endogenous stimulus response to hormone response to steroid hormone cellular response to organic substance cellular response to steroid hormone stimulus response to organic cyclic compound cellular response to hormone stimulus response to endogenous stimulus intracellular corticosteroid receptor signaling pathway cellular response to organic cyclic compound intracellular glucocorticoid receptor signaling pathway cellular response to chemical stimulus steroid hormone mediated signaling pathway response to chemical cellular response to lipid nuclear receptor signaling pathway intracellular steroid hormone receptor signaling pathway organelle organization cytoskeleton organization protein localization cellular localization establishment of protein localization nitrogen compound transport organic substance transport intracellular transport protein transport establishment of localization in cell cellular macromolecule localization macromolecule localization intracellular protein transport mitotic nuclear division regulation of cell cycle process organelle fission regulation of mitotic nuclear division regulation of organelle organization mitotic cell cycle regulation of mitotic cell cycle nuclear division mitotic cell cycle process regulation of nuclear division cell cycle regulation of cell cycle cell cycle process positive regulation of macromolecule biosynthetic process regulation of primary metabolic process nucleobase-containing compound metabolic process positive regulation of metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process positive regulation of nucleobase-containing compound metabolic process cellular metabolic process regulation of cellular metabolic process positive regulation of RNA metabolic process regulation of gene expression biosynthetic process organic cyclic compound biosynthetic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process positive regulation of DNA-templated transcription cellular biosynthetic process positive regulation of biological process RNA metabolic process positive regulation of cellular metabolic process RNA biosynthetic process nucleic acid biosynthetic process positive regulation of biosynthetic process positive regulation of cellular process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process positive regulation of RNA biosynthetic process positive regulation of cellular biosynthetic process	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol cell periphery plasma membrane membrane presynapse synapse cell junction neuron to neuron synapse asymmetric synapse cerebellar granule cell to Purkinje cell synapse glutamatergic synapse intracellular anatomical structure cytoplasm cellular anatomical entity cytosol cell periphery plasma membrane membrane presynapse synapse cell junction neuron to neuron synapse asymmetric synapse cerebellar granule cell to Purkinje cell synapse glutamatergic synapse excitatory synapse asymmetric, glutamatergic, excitatory synapse cell-cell junction intercalated disc anchoring junction cell-cell contact zone
E, F	Peptide from Protein numb homolog	protein binding binding alpha-catenin binding cell adhesion molecule binding cadherin binding beta-catenin binding	adherens junction organization cellular component organization cell junction organization cellular process cellular component organization or biogenesis cell-cell junction organization developmental process neuroblast proliferation multicellular organismal process cell population proliferation cellular developmental process nervous system development generation of neurons neurogenesis adherens junction organization cellular component organization cell junction organization cellular process cellular component organization or biogenesis cell-cell junction organization developmental process neuroblast proliferation multicellular organismal process cell population proliferation cellular developmental process nervous system development generation of neurons neurogenesis multicellular organism development cell differentiation anatomical structure development neural precursor cell proliferation system development protein localization regulation of localization cellular localization regulation of cellular process regulation of protein localization negative regulation of biological process negative regulation of protein localization to plasma membrane localization cellular macromolecule localization negative regulation of cellular process regulation of protein localization to membrane negative regulation of protein localization regulation of biological process protein localization to cell periphery protein localization to plasma membrane biological regulation localization within membrane regulation of protein localization to cell periphery protein localization to membrane negative regulation of protein localization to membrane regulation of cellular localization macromolecule localization negative regulation of protein localization to cell periphery regulation of protein localization to plasma membrane head development lateral ventricle development forebrain development animal organ development brain development central nervous system development ventricular system development telencephalon development axonogenesis cell projection organization plasma membrane bounded cell projection morphogenesis cell development neuron projection development cell morphogenesis cell projection morphogenesis neuron differentiation neuron projection morphogenesis plasma membrane bounded cell projection organization axon development cell morphogenesis involved in neuron differentiation anatomical structure morphogenesis neuron development regulation of cellular component organization cellular component biogenesis cellular component assembly regulation of postsynapse assembly cell junction assembly regulation of synapse structure or activity synapse organization regulation of synapse assembly regulation of biological quality postsynapse organization postsynapse assembly regulation of postsynapse organization regulation of cellular component biogenesis regulation of cell junction assembly regulation of synapse organization synapse assembly regulation of developmental process regulation of cell differentiation regulation of neuron differentiation positive regulation of locomotion regulation of cell motility positive regulation of biological process positive regulation of cell migration regulation of locomotion positive regulation of cell motility regulation of cell migration cell migration cell motility locomotion positive regulation of cellular process cell proliferation in forebrain neuroblast division neuroblast division in subventricular zone cell division positive regulation of nervous system development regulation of neurogenesis regulation of cell development regulation of nervous system development positive regulation of developmental process positive regulation of cell differentiation positive regulation of neurogenesis regulation of multicellular organismal process regulation of multicellular organismal development positive regulation of cell development positive regulation of multicellular organismal process regulation of cell morphogenesis regulation of dendrite morphogenesis positive regulation of cell projection organization regulation of cell projection organization positive regulation of cell morphogenesis regulation of dendrite development regulation of neuron projection development positive regulation of cellular component organization dendrite development regulation of anatomical structure morphogenesis positive regulation of dendrite morphogenesis dendrite morphogenesis regulation of plasma membrane bounded cell projection organization import into cell receptor internalization transport postsynaptic neurotransmitter receptor internalization regulation of receptor internalization vesicle-mediated transport vesicle-mediated transport in synapse establishment of localization in cell regulation of postsynaptic neurotransmitter receptor internalization regulation of endocytosis regulation of transport regulation of receptor-mediated endocytosis regulation of vesicle-mediated transport establishment of localization protein-containing complex localization neurotransmitter receptor internalization regulation of postsynaptic membrane neurotransmitter receptor levels postsynaptic endocytosis endocytosis receptor-mediated endocytosis	clathrin-coated pit membrane cellular anatomical entity endomembrane system apical part of cell membrane-bounded organelle intracellular anatomical structure intracellular vesicle intracellular membrane-bounded organelle intracellular organelle cytoplasmic vesicle organelle clathrin-coated vesicle coated vesicle clathrin-coated pit membrane cellular anatomical entity endomembrane system apical part of cell membrane-bounded organelle intracellular anatomical structure intracellular vesicle intracellular membrane-bounded organelle intracellular organelle cytoplasmic vesicle organelle clathrin-coated vesicle coated vesicle cytoplasm vesicle cell periphery plasma membrane basal part of cell basal plasma membrane plasma membrane region basolateral plasma membrane synapse cell junction glutamatergic synapse early endosome endosome neuron spine neuron projection dendritic spine plasma membrane bounded cell projection somatodendritic compartment cell projection postsynapse dendrite dendritic tree postsynaptic density neuron to neuron synapse postsynaptic specialization asymmetric synapse endosome membrane cytoplasmic vesicle membrane bounding membrane of organelle vesicle membrane organelle membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR023409	14-3-3 protein, conserved site	Conserved Site
A, B, C, D	IPR023410	14-3-3 domain	Domain
A, B, C, D	IPR036815	14-3-3 domain superfamily	Homologous Superfamily
A, B, C, D	IPR000308	14-3-3 protein	Family
E, F	IPR011993	PH-like domain superfamily	Homologous Superfamily
E, F	IPR006020	PTB/PI domain	Domain
E, F	IPR016698	Numb/numb-like	Family
E, F	IPR010449	NUMB domain	Domain

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
E, F	SEP	Parent Component: SER RESID: AA0037 PSI-MOD : O-phospho-L-serine MOD:00046

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.