5WFN

Revised model of leiomodin 2-mediated actin regulation (alternate refinement of PDB 4RWT)

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
C [auth B]	d5wfnb2	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Actin-like ATPase domain	Actin/HSP70	automated matches	Fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)
C [auth B]	d5wfnb1	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Actin-like ATPase domain	automated matches	automated matches	Fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)
A	d5wfna2	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Actin-like ATPase domain	Actin/HSP70	automated matches	Fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)
A	d5wfna1	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Actin-like ATPase domain	automated matches	automated matches	Fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
C [auth B]	SCOP2B Superfamily	Actin-like ATPases	8082531	3000092	SCOP2B (2022-06-29)
C [auth B]	SCOP2B Superfamily	Actin-like ATPases	8092415	3000092	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Actin-like ATPases	8082531	3000092	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Actin-like ATPases	8092415	3000092	SCOP2B (2022-06-29)
B [auth C]	SCOP2B Superfamily	RNI-like	8061612	3000990	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	RNI-like	8061612	3000990	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C [auth B]	Actin_1st	e5wfnB2	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Actin_1st	ECOD (1.6)
C [auth B]	Actin_2nd	e5wfnB1	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Actin_2nd	ECOD (1.6)
A	Actin_1st	e5wfnA2	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Actin_1st	ECOD (1.6)
A	Actin_2nd	e5wfnA1	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Actin_2nd	ECOD (1.6)
B [auth C]	LRR_8_4	e5wfnC1	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Leucine-rich repeats (From Topology)	T: Leucine-rich repeats	F: LRR_8_4	ECOD (1.6)
D	LRR_8_4	e5wfnD1	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Leucine-rich repeats (From Topology)	T: Leucine-rich repeats	F: LRR_8_4	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C [auth B]	3.30.420.40	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (4.3.0)
C [auth B]	3.90.640.10	Alpha Beta	Alpha-Beta Complex	Actin	Chain A, domain 4	CATH (4.3.0)
A	3.30.420.40	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (4.3.0)
A	3.90.640.10	Alpha Beta	Alpha-Beta Complex	Actin	Chain A, domain 4	CATH (4.3.0)
B [auth C]	3.80.10.10	Alpha Beta	Alpha-Beta Horseshoe	Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)	Ribonuclease Inhibitor	CATH (4.3.0)
D	3.80.10.10	Alpha Beta	Alpha-Beta Horseshoe	Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)	Ribonuclease Inhibitor	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C [auth B]	PF00022	Actin (Actin)	Actin	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C [auth B]	Actin-5C	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding hydrolase activity catalytic activity	maintenance of protein location protein localization localization cellular localization maintenance of protein location in cell cellular macromolecule localization macromolecule localization cellular process maintenance of location maintenance of location in cell mitotic cell cycle process cytoskeleton-dependent cytokinesis mitotic cytokinesis cytokinesis maintenance of protein location protein localization localization cellular localization maintenance of protein location in cell cellular macromolecule localization macromolecule localization cellular process maintenance of location maintenance of location in cell mitotic cell cycle process cytoskeleton-dependent cytokinesis mitotic cytokinesis cytokinesis cell division cell cycle mitotic cell cycle cell cycle process spermatid development multicellular organismal process spermatid differentiation sexual reproduction cellular developmental process spermatogenesis reproductive process cellularization cell development cellular process involved in reproduction in multicellular organism developmental process involved in reproduction gamete generation multicellular organism development cell differentiation anatomical structure development developmental process multicellular organismal reproductive process sperm individualization male gamete generation anatomical structure morphogenesis anatomical structure formation involved in morphogenesis germ cell development tube morphogenesis tube development tube formation protein-DNA complex organization cellular component organization protein-containing complex organization chromatin organization chromatin remodeling cellular component organization or biogenesis	membrane-bounded organelle ATPase complex protein-containing complex intracellular anatomical structure nucleus membrane-enclosed lumen nuclear protein-containing complex nuclear chromosome chromosome intracellular organelle lumen intracellular organelle SWI/SNF superfamily-type complex non-membrane-bounded organelle INO80-type complex membrane-bounded organelle ATPase complex protein-containing complex intracellular anatomical structure nucleus membrane-enclosed lumen nuclear protein-containing complex nuclear chromosome chromosome intracellular organelle lumen intracellular organelle SWI/SNF superfamily-type complex non-membrane-bounded organelle INO80-type complex catalytic complex protein-DNA complex chromatin intracellular membrane-bounded organelle cellular anatomical entity Ino80 complex organelle lumen organelle intracellular non-membrane-bounded organelle nuclear lumen brahma complex cytoskeleton cytoplasm
B [auth C], D	Leiomodin-2	cytoskeletal protein binding protein binding binding tropomyosin binding actin binding actin monomer binding	regulation of cellular process regulation of cellular component size negative regulation of biological process protein-containing complex disassembly pointed-end actin filament capping regulation of protein-containing complex disassembly actin filament depolymerization regulation of actin filament depolymerization cellular component biogenesis actin filament capping cellular component assembly actin filament-based process negative regulation of supramolecular fiber organization regulation of biological quality regulation of cellular process regulation of cellular component size negative regulation of biological process protein-containing complex disassembly pointed-end actin filament capping regulation of protein-containing complex disassembly actin filament depolymerization regulation of actin filament depolymerization cellular component biogenesis actin filament capping cellular component assembly actin filament-based process negative regulation of supramolecular fiber organization regulation of biological quality negative regulation of actin filament polymerization cellular component disassembly regulation of biological process negative regulation of cellular component organization actin filament organization regulation of organelle organization negative regulation of protein depolymerization negative regulation of protein-containing complex assembly regulation of protein polymerization regulation of supramolecular fiber organization supramolecular fiber organization regulation of cytoskeleton organization regulation of cellular component organization regulation of actin filament-based process cellular component organization regulation of protein-containing complex assembly negative regulation of protein-containing complex disassembly negative regulation of cellular process protein-containing complex assembly negative regulation of protein polymerization cellular component organization or biogenesis regulation of actin filament polymerization actin polymerization or depolymerization protein polymerization regulation of actin filament organization actin cytoskeleton organization actin filament polymerization protein-containing complex organization regulation of protein depolymerization negative regulation of cytoskeleton organization biological regulation regulation of actin polymerization or depolymerization regulation of cellular component biogenesis organelle organization negative regulation of organelle organization regulation of actin filament length regulation of actin cytoskeleton organization cellular process negative regulation of actin filament depolymerization protein depolymerization cytoskeleton organization regulation of anatomical structure size positive regulation of organelle organization positive regulation of cellular component organization positive regulation of biological process positive regulation of protein-containing complex assembly positive regulation of protein polymerization positive regulation of cellular process positive regulation of cellular component biogenesis positive regulation of cytoskeleton organization positive regulation of supramolecular fiber organization positive regulation of actin filament polymerization actin nucleation multicellular organismal process system process muscle contraction muscle system process cellular developmental process cellular component assembly involved in morphogenesis non-membrane-bounded organelle assembly cell development muscle cell differentiation muscle structure development striated muscle cell development cellular anatomical entity morphogenesis muscle cell development cell differentiation anatomical structure development developmental process actomyosin structure organization organelle assembly anatomical structure morphogenesis myofibril assembly anatomical structure formation involved in morphogenesis striated muscle cell differentiation sarcomere organization	M band intracellular anatomical structure cellular anatomical entity sarcomere intracellular organelle organelle intracellular non-membrane-bounded organelle contractile muscle fiber A band cytoplasm supramolecular fiber myofibril supramolecular polymer supramolecular complex M band intracellular anatomical structure cellular anatomical entity sarcomere intracellular organelle organelle intracellular non-membrane-bounded organelle contractile muscle fiber A band cytoplasm supramolecular fiber myofibril supramolecular polymer supramolecular complex non-membrane-bounded organelle cytoskeleton polymeric cytoskeletal fiber actin filament actin cytoskeleton striated muscle thin filament myofilament

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C [auth B]	IPR020902	Actin/actin-like conserved site	Conserved Site
A, C [auth B]	IPR004001	Actin, conserved site	Conserved Site
A, C [auth B]	IPR043129	ATPase, nucleotide binding domain	Homologous Superfamily
A, C [auth B]	IPR004000	Actin family	Family
B [auth C], D	IPR032675	Leucine-rich repeat domain superfamily	Homologous Superfamily
B [auth C], D	IPR004934	Tropomodulin	Family
B [auth C], D	IPR003124	WH2 domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
B [auth C], D	Pharos: Q6P5Q4	Nonorganic psychosis schizophrenia

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.