5UZ9

Cryo EM structure of anti-CRISPRs, AcrF1 and AcrF2, bound to type I-F crRNA-guided CRISPR surveillance complex

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
L	SCOP2B Superfamily	CRISPR-associated proteins	8052171	3001554	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF09611	e5uz9A1	A: a+b complex topology	X: Cas8-like	H: Cas8f/Csy1 (From Topology)	T: Cas8f/Csy1	F: PF09611	ECOD (1.6)
B	PF09614	e5uz9B1	A: a+b two layers	X: Alpha-beta plaits	H: CRISPR system Cas5 homologs (From Topology)	T: CRISPR system Cas5 homologs	F: PF09614	ECOD (1.6)
D	PF09615	e5uz9D1	A: a+b two layers	X: Alpha-beta plaits	H: Cas7-related	T: CRISPR-associated protein Cas7f/Csy3	F: PF09615	ECOD (1.6)
E	PF09615	e5uz9E1	A: a+b two layers	X: Alpha-beta plaits	H: Cas7-related	T: CRISPR-associated protein Cas7f/Csy3	F: PF09615	ECOD (1.6)
F	PF09615	e5uz9F1	A: a+b two layers	X: Alpha-beta plaits	H: Cas7-related	T: CRISPR-associated protein Cas7f/Csy3	F: PF09615	ECOD (1.6)
G	PF09615	e5uz9G1	A: a+b two layers	X: Alpha-beta plaits	H: Cas7-related	T: CRISPR-associated protein Cas7f/Csy3	F: PF09615	ECOD (1.6)
H	PF09615	e5uz9H1	A: a+b two layers	X: Alpha-beta plaits	H: Cas7-related	T: CRISPR-associated protein Cas7f/Csy3	F: PF09615	ECOD (1.6)
C	PF09615	e5uz9C1	A: a+b two layers	X: Alpha-beta plaits	H: Cas7-related	T: CRISPR-associated protein Cas7f/Csy3	F: PF09615	ECOD (1.6)
I	PF20829	e5uz9I1	A: a+b two layers	X: dsRBD-like	H: Anti-CRISPR protein Acr30-35/AcrF1 (From Topology)	T: Anti-CRISPR protein Acr30-35/AcrF1	F: PF20829	ECOD (1.6)
J	PF20829	e5uz9J1	A: a+b two layers	X: dsRBD-like	H: Anti-CRISPR protein Acr30-35/AcrF1 (From Topology)	T: Anti-CRISPR protein Acr30-35/AcrF1	F: PF20829	ECOD (1.6)
K	F_UNCLASSIFIED	e5uz9K1	A: a+b two layers	X: dsRBD-like	H: Anti-CRISPR protein AcrF2 (From Topology)	T: Anti-CRISPR protein AcrF2	F: F_UNCLASSIFIED	ECOD (1.6)
L	PF09618	e5uz9L2	A: a+b two layers	X: Alpha-beta plaits	H: CRISPR transcript (pre-crRNA) processing endoribonuclease-related (From Topology)	T: CRISPR transcript (pre-crRNA) processing endoribonuclease-related	F: PF09618	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
I	3.30.67.30	Alpha Beta	2-Layer Sandwich	Viral Envelope Glycoprotein	domain 2	CATH (4.3.0)
J	3.30.67.30	Alpha Beta	2-Layer Sandwich	Viral Envelope Glycoprotein	domain 2	CATH (4.3.0)
L	3.30.70.2540	Alpha Beta	2-Layer Sandwich	Alpha-Beta Plaits	CRISPR-associated endoribonuclease Cas6/Csy4	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF09611	CRISPR-associated protein (Cas_Csy1) (Cas_Csy1)	CRISPR-associated protein (Cas_Csy1)	-	Family
B	PF09614	CRISPR-associated protein (Cas_Csy2) (Cas_Csy2)	CRISPR-associated protein (Cas_Csy2)	-	Family
C, D, E, F, G C, D, E, F, G, H	PF09615	CRISPR-associated protein (Cas_Csy3) (Cas_Csy3)	CRISPR-associated protein (Cas_Csy3)	-	Family
I, J	PF20829	Anti-CRISPR protein Acr30-35/AcrF1 (Acr30-35_AcrF1)	Anti-CRISPR protein Acr30-35/AcrF1	-	Family
L	PF09618	CRISPR-associated protein (Cas_Csy4) (Cas_Csy4)	CRISPR-associated protein (Cas_Csy4)	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	CRISPR-associated protein Csy1	-	biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to stress response to external biotic stimulus response to virus	-
B	CRISPR-associated protein Csy2	-	biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to stress response to external biotic stimulus response to virus	-
C, D, E, F, G C, D, E, F, G, H	CRISPR-associated protein Csy3	-	biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to stress response to external biotic stimulus response to virus	-
I, J	Anti-CRISPR protein Acr30-35	-	-	-
K	Anti-CRISPR protein 30	-	symbiont-mediated perturbation of host defense response response to host defenses biological process involved in interspecies interaction between organisms response to stimulus response to other organism symbiont-mediated perturbation of host immune response symbiont-mediated suppression of host CRISPR-cas system response to defenses of other organism symbiont-mediated perturbation of host defenses response to external stimulus response to biotic stimulus biological process involved in interaction with host biological process involved in symbiotic interaction symbiont-mediated suppression of host immune response symbiont-mediated suppression of host innate immune response symbiont-mediated perturbation of host defense response response to host defenses biological process involved in interspecies interaction between organisms response to stimulus response to other organism symbiont-mediated perturbation of host immune response symbiont-mediated suppression of host CRISPR-cas system response to defenses of other organism symbiont-mediated perturbation of host defenses response to external stimulus response to biotic stimulus biological process involved in interaction with host biological process involved in symbiotic interaction symbiont-mediated suppression of host immune response symbiont-mediated suppression of host innate immune response modulation by symbiont of host process response to host immune response symbiont-mediated suppression of host defenses symbiont-mediated perturbation of host innate immune response response to host response to external biotic stimulus modulation of process of another organism	-
L	CRISPR-associated endonuclease Cas6/Csy4	endonuclease activity nuclease activity hydrolase activity, acting on ester bonds hydrolase activity catalytic activity RNA binding binding organic cyclic compound binding nucleic acid binding	biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to stress response to external biotic stimulus response to virus maintenance of CRISPR repeat elements chromosome organization biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to stress response to external biotic stimulus response to virus maintenance of CRISPR repeat elements chromosome organization primary metabolic process DNA metabolic process maintenance of DNA repeat elements nucleic acid metabolic process cellular component organization nucleobase-containing compound metabolic process organic cyclic compound metabolic process organelle organization organic substance metabolic process cellular process metabolic process cellular component organization or biogenesis macromolecule metabolic process	-
M	CRISPR RNA (60-MER)	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR013397	Type I-F CRISPR-associated protein Csy1	Family
B	IPR013398	Type I-F CRISPR-associated protein Csy2	Family
C, D, E, F, G C, D, E, F, G, H	IPR013399	Type I-F CRISPR-associated protein Csy3	Family
I, J	IPR049088	Anti-CRISPR protein Acr30-35/AcrF1	Family
L	IPR013396	CRISPR-associated endoribonuclease Cas6/Csy4, subtype I-F/YPEST	Family
L	IPR042564	CRISPR-associated endoribonuclease Cas6/Csy4, subtype I-F/YPEST superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.