Annotations: 5TCS

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Ndc80/Hec family	8051980	4004024	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Calponin-homology domain CH-domain	8051981	3000264	SCOP2 (2022-06-29)
B	SCOP2 Family	Nuf2-like	8044874	4004186	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Calponin-homology domain CH-domain	8053600	3000264	SCOP2 (2022-06-29)
C	SCOP2B Superfamily	RWD domain-like	8041319	3000774	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	RWD domain-like	8041316	3000774	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF03801	e5tcsA1	A: alpha bundles	X: CH domain-like	H: Calponin-homology domain-like (From Topology)	T: Calponin-homology domain-like	F: PF03801	ECOD (1.6)
A	PF18077	e5tcsA2	A: extended segments	X: Helical hairpin in Ndc80 (From Topology)	H: Helical hairpin in Ndc80 (From Topology)	T: Helical hairpin in Ndc80	F: PF18077	ECOD (1.6)
B	PF03800	e5tcsB1	A: alpha bundles	X: CH domain-like	H: Calponin-homology domain-like (From Topology)	T: Calponin-homology domain-like	F: PF03800	ECOD (1.6)
C	PF08286	e5tcsC1	A: a+b two layers	X: Kinetochore globular domain-like (From Topology)	H: Kinetochore globular domain-like (From Topology)	T: Kinetochore globular domain-like	F: PF08286	ECOD (1.6)
D	F_UNCLASSIFIED	e5tcsD1	A: a+b two layers	X: Kinetochore globular domain-like (From Topology)	H: Kinetochore globular domain-like (From Topology)	T: Kinetochore globular domain-like	F: F_UNCLASSIFIED	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
D	3.30.457.50	Alpha Beta	2-Layer Sandwich	Copper Amine Oxidase	Chain A, domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF18077	Domain of unknown function (DUF5595) (DUF5595)	Domain of unknown function (DUF5595)	This domain is found in Nude C 80 (Ndc80) proteins which can be found in species such as Homo sapiens. Ndc80 protein complexes are a core component of the end-on attachment sites for kinetochore microtubules. Ndc80 is also known as Hec1, for highly e ...	This domain is found in Nude C 80 (Ndc80) proteins which can be found in species such as Homo sapiens. Ndc80 protein complexes are a core component of the end-on attachment sites for kinetochore microtubules. Ndc80 is also known as Hec1, for highly expressed in cancer 1 [1].	Domain
A	PF03801	HEC/Ndc80p family (Ndc80_HEC)	HEC/Ndc80p family	-	Family
B	PF03800	Nuf2 family (Nuf2)	Nuf2 family	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Kinetochore protein NDC80	identical protein binding protein binding binding	protein localization cellular localization protein localization to chromosome, centromeric region localization protein localization to kinetochore protein localization to chromosome protein localization to condensed chromosome protein localization to organelle cellular macromolecule localization macromolecule localization cellular process establishment of organelle localization mitotic metaphase chromosome alignment mitotic cell cycle protein localization cellular localization protein localization to chromosome, centromeric region localization protein localization to kinetochore protein localization to chromosome protein localization to condensed chromosome protein localization to organelle cellular macromolecule localization macromolecule localization cellular process establishment of organelle localization mitotic metaphase chromosome alignment mitotic cell cycle metaphase chromosome alignment cellular component organization chromosome localization organelle localization establishment of localization in cell mitotic sister chromatid segregation cell cycle cellular component organization or biogenesis chromosome segregation cell cycle process mitotic nuclear division attachment of spindle microtubules to kinetochore organelle fission attachment of mitotic spindle microtubules to kinetochore chromosome organization sister chromatid segregation establishment of localization organelle organization nuclear division mitotic cell cycle process nuclear chromosome segregation establishment of chromosome localization cell division sister chromatid biorientation mitotic sister chromatid biorientation	intracellular anatomical structure chromosome cellular anatomical entity chromosomal region kinetochore intracellular organelle organelle intracellular non-membrane-bounded organelle chromosome, centromeric region condensed chromosome, centromeric region supramolecular complex condensed chromosome non-membrane-bounded organelle Ndc80 complex intracellular anatomical structure chromosome cellular anatomical entity chromosomal region kinetochore intracellular organelle organelle intracellular non-membrane-bounded organelle chromosome, centromeric region condensed chromosome, centromeric region supramolecular complex condensed chromosome non-membrane-bounded organelle Ndc80 complex protein-containing complex outer kinetochore membrane-bounded organelle nucleus intracellular membrane-bounded organelle
B	Kinetochore protein NUF2	protein-containing complex binding binding	establishment of organelle localization cellular localization mitotic metaphase chromosome alignment mitotic cell cycle metaphase chromosome alignment cellular component organization localization chromosome localization organelle localization establishment of localization in cell mitotic sister chromatid segregation cell cycle cellular component organization or biogenesis chromosome segregation establishment of organelle localization cellular localization mitotic metaphase chromosome alignment mitotic cell cycle metaphase chromosome alignment cellular component organization localization chromosome localization organelle localization establishment of localization in cell mitotic sister chromatid segregation cell cycle cellular component organization or biogenesis chromosome segregation cell cycle process mitotic nuclear division attachment of spindle microtubules to kinetochore organelle fission attachment of mitotic spindle microtubules to kinetochore chromosome organization sister chromatid segregation establishment of localization organelle organization nuclear division mitotic cell cycle process nuclear chromosome segregation cellular process establishment of chromosome localization cell division microtubule cytoskeleton organization mitotic spindle organization microtubule-based process cytoskeleton organization microtubule cytoskeleton organization involved in mitosis spindle organization meiotic chromosome segregation sexual reproduction reproductive process meiotic cell cycle meiotic cell cycle process meiotic nuclear division kinetochore organization	intracellular anatomical structure chromosome cellular anatomical entity chromosomal region kinetochore intracellular organelle organelle intracellular non-membrane-bounded organelle chromosome, centromeric region condensed chromosome, centromeric region supramolecular complex condensed chromosome non-membrane-bounded organelle microtubule organizing center intracellular anatomical structure chromosome cellular anatomical entity chromosomal region kinetochore intracellular organelle organelle intracellular non-membrane-bounded organelle chromosome, centromeric region condensed chromosome, centromeric region supramolecular complex condensed chromosome non-membrane-bounded organelle microtubule organizing center cytoskeleton spindle pole body microtubule cytoskeleton Ndc80 complex protein-containing complex outer kinetochore polymeric cytoskeletal fiber spindle spindle microtubule supramolecular fiber microtubule supramolecular polymer membrane-bounded organelle nucleus intracellular membrane-bounded organelle
C	Kinetochore protein SPC24	identical protein binding protein binding binding	establishment of organelle localization cellular localization attachment of spindle microtubules to kinetochore sister chromatid biorientation chromosome organization sister chromatid segregation establishment of localization cellular component organization metaphase chromosome alignment localization organelle organization chromosome localization organelle localization establishment of localization in cell nuclear chromosome segregation establishment of organelle localization cellular localization attachment of spindle microtubules to kinetochore sister chromatid biorientation chromosome organization sister chromatid segregation establishment of localization cellular component organization metaphase chromosome alignment localization organelle organization chromosome localization organelle localization establishment of localization in cell nuclear chromosome segregation cellular process cellular component organization or biogenesis cell cycle establishment of chromosome localization chromosome segregation cell cycle process centromere localization centromere clustering protein-containing complex localization cell division	Ndc80 complex protein-containing complex intracellular anatomical structure chromosome cellular anatomical entity chromosomal region kinetochore intracellular organelle organelle intracellular non-membrane-bounded organelle chromosome, centromeric region outer kinetochore condensed chromosome, centromeric region supramolecular complex Ndc80 complex protein-containing complex intracellular anatomical structure chromosome cellular anatomical entity chromosomal region kinetochore intracellular organelle organelle intracellular non-membrane-bounded organelle chromosome, centromeric region outer kinetochore condensed chromosome, centromeric region supramolecular complex condensed chromosome non-membrane-bounded organelle membrane-bounded organelle nucleus intracellular membrane-bounded organelle
D	Kinetochore protein SPC25	-	localization centromere localization chromosome localization organelle localization centromere clustering protein-containing complex localization cellular process cell division cell cycle chromosome segregation cell cycle process	Ndc80 complex protein-containing complex intracellular anatomical structure chromosome cellular anatomical entity chromosomal region kinetochore intracellular organelle organelle intracellular non-membrane-bounded organelle chromosome, centromeric region outer kinetochore condensed chromosome, centromeric region supramolecular complex Ndc80 complex protein-containing complex intracellular anatomical structure chromosome cellular anatomical entity chromosomal region kinetochore intracellular organelle organelle intracellular non-membrane-bounded organelle chromosome, centromeric region outer kinetochore condensed chromosome, centromeric region supramolecular complex condensed chromosome non-membrane-bounded organelle membrane-bounded organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR040967	Domain of unknown function DUF5595	Domain
A	IPR038273	Ndc80 domain superfamily	Homologous Superfamily
A	IPR005550	Kinetochore protein Ndc80	Family
B	IPR038275	Nuf2, N-terminal domain superfamily	Homologous Superfamily
B	IPR005549	Kinetochore protein Nuf2, N-terminal	Domain
C	IPR013252	Kinetochore-Ndc80 subunit Spc24	Family
C	IPR038066	Spc24, Fungi, globular domain superfamily	Homologous Superfamily
D	IPR038066	Spc24, Fungi, globular domain superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.