5T1O

Solution-state NMR and SAXS structural ensemble of NPr (1-85) in complex with EIN-Ntr (170-424)

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d5t1oa_	Alpha and beta proteins (a+b)	HPr-like	HPr-like	automated matches	automated matches	(Escherichia coli O157:H7 ) [TaxId: 83334 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	HPr-like	8098384	3000259	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	PEP-utilisers_N	e5t1oB1	A: alpha arrays	X: Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain (From Topology)	H: Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain (From Topology)	T: Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain	F: PEP-utilisers_N	ECOD (1.6)
B	PEP-utilizers	e5t1oB2	A: a/b three-layered sandwiches	X: The swivelling beta/beta/alpha domains	H: The swivelling beta/beta/alpha domain (From Topology)	T: The swivelling beta/beta/alpha domain	F: PEP-utilizers	ECOD (1.6)
A	PTS-HPr	e5t1oA1	A: a+b two layers	X: HPr-like (From Topology)	H: HPr-like (From Topology)	T: HPr-like	F: PTS-HPr	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.1340.10	Alpha Beta	2-Layer Sandwich	Histidine-containing Protein	Chain: A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
B	PF00391	PEP-utilising enzyme, mobile domain (PEP-utilizers)	PEP-utilising enzyme, mobile domain	This domain is a "swivelling" beta/beta/alpha domain which is thought to be mobile in all proteins known to contain it.	Domain
B	PF02896	PEP-utilising enzyme, PEP-binding domain (PEP-utilizers_C)	PEP-utilising enzyme, PEP-binding domain	This entry represents a TIM barrel domain found at the C terminus of a number of PEP (phosphoenolpyruvate)-utilising proteins. In PPDK (Pyruvate phosphate dikinase) this C-terminal domain has been shown to be a PEP-binding domain [1].	Domain
B	PF05524	PEP-utilising enzyme, N-terminal (PEP-utilisers_N)	PEP-utilising enzyme, N-terminal	-	Family
A	PF00381	PTS HPr component phosphorylation site (PTS-HPr)	PTS HPr component phosphorylation site	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
B	Phosphoenolpyruvate-protein phosphotransferase PtsP	kinase activity transferase activity catalytic activity transferase activity, transferring phosphorus-containing groups phosphoenolpyruvate-protein phosphotransferase activity phosphotransferase activity, nitrogenous group as acceptor cation binding ion binding binding metal ion binding small molecule binding	import into cell phosphoenolpyruvate-dependent sugar phosphotransferase system transport organic substance transport import across plasma membrane carbohydrate import across plasma membrane carbohydrate transport establishment of localization transmembrane transport localization cellular process carbohydrate transmembrane transport response to organic substance response to stimulus import into cell phosphoenolpyruvate-dependent sugar phosphotransferase system transport organic substance transport import across plasma membrane carbohydrate import across plasma membrane carbohydrate transport establishment of localization transmembrane transport localization cellular process carbohydrate transmembrane transport response to organic substance response to stimulus response to organonitrogen compound response to chemical response to nitrogen compound phosphorylation cellular metabolic process phosphate-containing compound metabolic process metabolic process phosphorus metabolic process	intracellular anatomical structure cytoplasm cellular anatomical entity
A	Phosphocarrier protein NPr	transferase activity catalytic activity transferase activity, transferring phosphorus-containing groups	import into cell phosphoenolpyruvate-dependent sugar phosphotransferase system transport organic substance transport import across plasma membrane carbohydrate import across plasma membrane carbohydrate transport establishment of localization transmembrane transport localization cellular process carbohydrate transmembrane transport response to organic substance response to stimulus import into cell phosphoenolpyruvate-dependent sugar phosphotransferase system transport organic substance transport import across plasma membrane carbohydrate import across plasma membrane carbohydrate transport establishment of localization transmembrane transport localization cellular process carbohydrate transmembrane transport response to organic substance response to stimulus response to organonitrogen compound response to chemical response to nitrogen compound	intracellular anatomical structure cytoplasm cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
B	IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily	Homologous Superfamily
B	IPR008279	PEP-utilising enzyme, mobile domain	Domain
B	IPR018274	PEP-utilising enzyme, active site	Active Site
B	IPR036618	PtsI, HPr-binding domain superfamily	Homologous Superfamily
B	IPR006318	Phosphotransferase system, enzyme I-like	Domain
B	IPR023151	PEP-utilising enzyme, conserved site	Conserved Site
B	IPR036637	Phosphohistidine domain superfamily	Homologous Superfamily
B	IPR003018	GAF domain	Domain
B	IPR029016	GAF-like domain superfamily	Homologous Superfamily
B	IPR040442	Pyruvate kinase-like domain superfamily	Homologous Superfamily
B	IPR008731	Phosphotransferase system, enzyme I N-terminal	Domain
B	IPR000121	PEP-utilising enzyme, C-terminal	Domain
A	IPR000032	Phosphocarrier protein HPr-like	Domain
A	IPR002114	Phosphotransferase system, HPr serine phosphorylation site	PTM
A	IPR035895	HPr-like superfamily	Homologous Superfamily
A	IPR001020	Phosphotransferase system, HPr histidine phosphorylation site	PTM

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.