5ON3

Quaternary complex of mutant T252A of E. coli leucyl-tRNA synthetase with tRNA(leu), leucyl-adenylate analogue, and post-transfer editing analogue of leucine in the aminoacylation conformation

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	tRNA-synt_1_2nd	e5on3A2	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: acid protease	F: tRNA-synt_1_2nd	ECOD (1.6)
A	Anticodon_1,tRNA-synt_1_1st	e5on3A6	A: alpha bundles	X: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases (From Topology)	H: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases (From Topology)	T: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases	F: Anticodon_1,tRNA-synt_1_1st	ECOD (1.6)
A	leuS_3rd	e5on3A5	A: a+b two layers	X: beta-Grasp	H: beta-grasp fold domain in leucine-tRNA ligase (From Topology)	T: beta-grasp fold domain in leucine-tRNA ligase	F: leuS_3rd	ECOD (1.6)
A	tRNA-synt_1_1st	e5on3A7	A: a/b three-layered sandwiches	X: HUP domain-like	H: HUP domains (From Topology)	T: HUP domains	F: tRNA-synt_1_1st	ECOD (1.6)
A	tRNA-synt_1_3rd	e5on3A4	A: few secondary structure elements	X: Rubredoxin-like	H: Rubredoxin-related	T: Rubredoxin-related	F: tRNA-synt_1_3rd	ECOD (1.6)
A	tRNA-synt_1g	e5on3A3	A: few secondary structure elements	X: Rubredoxin-like	H: Rubredoxin-related	T: Rubredoxin-related	F: tRNA-synt_1g	ECOD (1.6)
A	UNK_F_TYPE	e5on3A1	A: few secondary structure elements	X: Rubredoxin-like	H: Rubredoxin-like domain in leucyl-tRNA synthetase (LS1) (From Topology)	T: Rubredoxin-like domain in leucyl-tRNA synthetase (LS1)	F: UNK_F_TYPE	ECOD (1.6)
C [auth D]	tRNA-synt_1_2nd	e5on3D5	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: acid protease	F: tRNA-synt_1_2nd	ECOD (1.6)
C [auth D]	Anticodon_1,tRNA-synt_1_1st	e5on3D6	A: alpha bundles	X: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases (From Topology)	H: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases (From Topology)	T: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases	F: Anticodon_1,tRNA-synt_1_1st	ECOD (1.6)
C [auth D]	leuS_3rd	e5on3D4	A: a+b two layers	X: beta-Grasp	H: beta-grasp fold domain in leucine-tRNA ligase (From Topology)	T: beta-grasp fold domain in leucine-tRNA ligase	F: leuS_3rd	ECOD (1.6)
C [auth D]	tRNA-synt_1_1st	e5on3D7	A: a/b three-layered sandwiches	X: HUP domain-like	H: HUP domains (From Topology)	T: HUP domains	F: tRNA-synt_1_1st	ECOD (1.6)
C [auth D]	tRNA-synt_1_3rd	e5on3D2	A: few secondary structure elements	X: Rubredoxin-like	H: Rubredoxin-related	T: Rubredoxin-related	F: tRNA-synt_1_3rd	ECOD (1.6)
C [auth D]	tRNA-synt_1g	e5on3D3	A: few secondary structure elements	X: Rubredoxin-like	H: Rubredoxin-related	T: Rubredoxin-related	F: tRNA-synt_1g	ECOD (1.6)
C [auth D]	UNK_F_TYPE	e5on3D1	A: few secondary structure elements	X: Rubredoxin-like	H: Rubredoxin-like domain in leucyl-tRNA synthetase (LS1) (From Topology)	T: Rubredoxin-like domain in leucyl-tRNA synthetase (LS1)	F: UNK_F_TYPE	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C [auth D]	PF13603	Leucyl-tRNA synthetase, editing domain (tRNA-synt_1_2)	Leucyl-tRNA synthetase, editing domain	This entry represents the editing domain in Leucine-tRNA ligase or Leucyl-tRNA synthetase, EC:6.1.1.4.	Domain
A, C [auth D]	PF09334	tRNA synthetases class I (M) (tRNA-synt_1g)	tRNA synthetases class I (M)	-	Family
A, C [auth D]	PF08264	Anticodon-binding domain of tRNA ligase (Anticodon_1)	Anticodon-binding domain of tRNA ligase	This domain is found mainly hydrophobic tRNA synthetases. The domain binds to the anticodon of the tRNA ligase.	Domain
A, C [auth D]	PF00133	tRNA synthetases class I (I, L, M and V) (tRNA-synt_1)	tRNA synthetases class I (I, L, M and V)	Other tRNA synthetase sub-families are too dissimilar to be included.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C [auth D]	Leucine--tRNA ligase	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding ligase activity, forming carbon-oxygen bonds aminoacyl-tRNA ligase activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid leucine-tRNA ligase activity catalytic activity, acting on a tRNA catalytic activity ligase activity hydrolase activity, acting on ester bonds carboxylic ester hydrolase activity aminoacyl-tRNA editing activity hydrolase activity	amino acid activation tRNA aminoacylation cellular biosynthetic process organonitrogen compound biosynthetic process RNA metabolic process amino acid metabolic process nucleobase-containing compound metabolic process organic substance metabolic process organic substance biosynthetic process protein metabolic process leucyl-tRNA aminoacylation organonitrogen compound metabolic process cellular metabolic process primary metabolic process amino acid activation tRNA aminoacylation cellular biosynthetic process organonitrogen compound biosynthetic process RNA metabolic process amino acid metabolic process nucleobase-containing compound metabolic process organic substance metabolic process organic substance biosynthetic process protein metabolic process leucyl-tRNA aminoacylation organonitrogen compound metabolic process cellular metabolic process primary metabolic process translation gene expression tRNA aminoacylation for protein translation macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process organic cyclic compound metabolic process biosynthetic process ncRNA metabolic process metabolic process cellular process macromolecule metabolic process	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol
B, D [auth E]	tRNA(leu)	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C [auth D]	IPR025709	Leucyl-tRNA synthetase, editing domain	Domain
A, C [auth D]	IPR001412	Aminoacyl-tRNA synthetase, class I, conserved site	Conserved Site
A, C [auth D]	IPR015413	Methionyl/Leucyl tRNA synthetase	Domain
A, C [auth D]	IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	Homologous Superfamily
A, C [auth D]	IPR002300	Aminoacyl-tRNA synthetase, class Ia	Domain
A, C [auth D]	IPR009008	Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain	Homologous Superfamily
A, C [auth D]	IPR009080	Aminoacyl-tRNA synthetase, class Ia, anticodon-binding	Homologous Superfamily
A, C [auth D]	IPR013155	Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding	Domain
A, C [auth D]	IPR002302	Leucine-tRNA ligase	Family

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.