Annotations: 5NRO

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Actin-like ATPases	8044476	3000092	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Heat shock protein 70kD (HSP70) C-terminal subdomain	8035544	3000668	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	heat shock protein 70kd (hsp70) peptide-binding domain	8035545	3000642	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Actin-like ATPases	8044475	3000092	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Chaperone J-domain	8036317	3000721	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	HSP70_2nd	e5nroA2	A: beta sandwiches	X: Heat shock protein 70kD (HSP70), peptide-binding domain (From Topology)	H: Heat shock protein 70kD (HSP70), peptide-binding domain (From Topology)	T: Heat shock protein 70kD (HSP70), peptide-binding domain	F: HSP70_2nd	ECOD (1.6)
A	HSP70_3rd	e5nroA3	A: alpha bundles	X: immunoglobulin/albumin-binding domain-like	H: Heat shock protein 70kD (HSP70), C-terminal subdomain (From Topology)	T: Heat shock protein 70kD (HSP70), C-terminal subdomain	F: HSP70_3rd	ECOD (1.6)
A	HSP70_1st	e5nroA1	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: HSP70_1st	ECOD (1.6)
A	MreB_Mbl_C	e5nroA4	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: MreB_Mbl_C	ECOD (1.6)
B	DnaJ	e5nroB1	A: alpha bundles	X: Chaperone J-domain (From Topology)	H: Chaperone J-domain (From Topology)	T: Chaperone J-domain	F: DnaJ	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.420.40	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (4.3.0)
A	3.90.640.10	Alpha Beta	Alpha-Beta Complex	Actin	Chain A, domain 4	CATH (4.3.0)
A	2.60.34.10	Mainly Beta	Sandwich	Substrate Binding Domain Of DNAk	Chain A, domain 1	CATH (4.3.0)
A	1.20.1270.10	Mainly Alpha	Up-down Bundle	Substrate Binding Domain Of Dnak	Chain:A	CATH (4.3.0)
B	1.10.287.110	Mainly Alpha	Orthogonal Bundle	Helix Hairpins	DnaJ domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00012	Hsp70 protein (HSP70)	Hsp70 protein	-	Family
B	PF00226	DnaJ domain (DnaJ)	DnaJ domain	DnaJ domains (J-domains) are associated with hsp70 heat-shock system and it is thought that this domain mediates the interaction. DnaJ-domain is therefore part of a chaperone (protein folding) system. The T-antigens, although not in Prosite are conf ...	DnaJ domains (J-domains) are associated with hsp70 heat-shock system and it is thought that this domain mediates the interaction. DnaJ-domain is therefore part of a chaperone (protein folding) system. The T-antigens, although not in Prosite are confirmed as DnaJ containing domains from literature [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Chaperone protein DnaK	protein binding binding heat shock protein binding ATP-dependent protein folding chaperone protein folding chaperone ATP-dependent activity protein-folding chaperone binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding protein binding binding heat shock protein binding ATP-dependent protein folding chaperone protein folding chaperone ATP-dependent activity protein-folding chaperone binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding ADP binding unfolded protein binding sigma factor antagonist activity transcription corepressor activity transcription regulator activity transcription coregulator activity protein-macromolecule adaptor activity molecular adaptor activity pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity cation binding transition metal ion binding zinc ion binding metal ion binding	protein refolding organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process protein folding biosynthetic process organic substance biosynthetic process metabolic process cellular process protein refolding organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process protein folding biosynthetic process organic substance biosynthetic process metabolic process cellular process protein metabolic process macromolecule metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA replication DNA metabolic process nucleic acid metabolic process cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein-containing complex assembly cellular component organization or biogenesis protein unfolding chaperone cofactor-dependent protein refolding chaperone-mediated protein folding 'de novo' protein folding 'de novo' post-translational protein folding cellular response to stimulus response to organic substance cellular response to topologically incorrect protein response to stimulus cellular response to unfolded protein response to unfolded protein cellular response to chemical stimulus cellular response to stress response to chemical response to stress response to topologically incorrect protein cellular response to organic substance response to heat response to abiotic stimulus response to temperature stimulus response to copper ion response to metal ion stress response to copper ion stress response to metal ion response to inorganic substance	membrane cellular anatomical entity intracellular anatomical structure cytoplasm cytosol cell periphery plasma membrane inclusion body protein-containing complex
B	Chaperone protein DnaJ	isomerase activity intramolecular oxidoreductase activity catalytic activity, acting on a protein intramolecular oxidoreductase activity, transposing S-S bonds catalytic activity protein disulfide isomerase activity protein binding binding heat shock protein binding protein-folding chaperone binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding isomerase activity intramolecular oxidoreductase activity catalytic activity, acting on a protein intramolecular oxidoreductase activity, transposing S-S bonds catalytic activity protein disulfide isomerase activity protein binding binding heat shock protein binding protein-folding chaperone binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding unfolded protein binding identical protein binding protein homodimerization activity protein dimerization activity oxidoreductase activity, acting on a sulfur group of donors disulfide oxidoreductase activity oxidoreductase activity protein-disulfide reductase activity sigma factor antagonist activity transcription corepressor activity transcription regulator activity transcription coregulator activity protein-macromolecule adaptor activity molecular adaptor activity cation binding transition metal ion binding zinc ion binding metal ion binding	protein refolding organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process protein folding biosynthetic process organic substance biosynthetic process metabolic process cellular process protein refolding organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process protein folding biosynthetic process organic substance biosynthetic process metabolic process cellular process protein metabolic process macromolecule metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA replication DNA metabolic process nucleic acid metabolic process cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein-containing complex assembly cellular component organization or biogenesis viral process protein unfolding chaperone cofactor-dependent protein refolding chaperone-mediated protein folding 'de novo' protein folding 'de novo' post-translational protein folding response to stimulus response to stress response to heat response to abiotic stimulus response to temperature stimulus	membrane cellular anatomical entity intracellular anatomical structure cytoplasm cytosol protein-containing complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR018181	Heat shock protein 70, conserved site	Conserved Site
A	IPR043129	ATPase, nucleotide binding domain	Homologous Superfamily
A	IPR012725	Chaperone DnaK	Family
A	IPR029048	Heat shock protein 70kD, C-terminal domain superfamily	Homologous Superfamily
A	IPR029047	Heat shock protein 70kD, peptide-binding domain superfamily	Homologous Superfamily
A	IPR013126	Heat shock protein 70 family	Family
B	IPR001305	Heat shock protein DnaJ, cysteine-rich domain	Domain
B	IPR001623	DnaJ domain	Domain
B	IPR012724	Chaperone DnaJ	Family
B	IPR018253	DnaJ domain, conserved site	Conserved Site
B	IPR002939	Chaperone DnaJ, C-terminal	Domain
B	IPR036869	Chaperone J-domain superfamily	Homologous Superfamily
B	IPR008971	HSP40/DnaJ peptide-binding	Homologous Superfamily
B	IPR036410	Heat shock protein DnaJ, cysteine-rich domain superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.