5MZ6

Cryo-EM structure of a Separase-Securin complex from Caenorhabditis elegans at 3.8 A resolution

External Resource: Annotation

Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth 1]	PF03568	Peptidase family C50 (Peptidase_C50)	Peptidase family C50	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth 1]	SEParase	cysteine-type peptidase activity peptidase activity catalytic activity, acting on a protein cysteine-type endopeptidase activity hydrolase activity catalytic activity endopeptidase activity	mitotic cell cycle process cytoskeleton-dependent cytokinesis mitotic cytokinesis cellular process cytokinesis cell division cell cycle mitotic cell cycle cell cycle process mitotic nuclear division organelle fission chromosome organization sister chromatid segregation cellular component organization mitotic cell cycle process cytoskeleton-dependent cytokinesis mitotic cytokinesis cellular process cytokinesis cell division cell cycle mitotic cell cycle cell cycle process mitotic nuclear division organelle fission chromosome organization sister chromatid segregation cellular component organization organelle organization nuclear division chromosome separation mitotic sister chromatid segregation nuclear chromosome segregation mitotic sister chromatid separation cellular component organization or biogenesis chromosome segregation sexual reproduction reproductive process meiotic cell cycle multicellular organismal reproductive process female gamete generation meiotic cell cycle process meiotic cytokinesis gamete generation female meiotic nuclear division polar body extrusion after meiotic divisions meiotic nuclear division protein localization localization cellular localization cellular macromolecule localization macromolecule localization meiotic chromosome segregation meiotic chromosome separation developmental process multicellular organismal process regulation of multicellular organismal development regulation of biological process regulation of developmental process biological regulation nematode larval development larval development multicellular organism development regulation of post-embryonic development regulation of nematode larval development anatomical structure development post-embryonic development regulation of multicellular organismal process organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process biosynthetic process proteolysis organic substance biosynthetic process protein processing metabolic process protein metabolic process macromolecule metabolic process mitotic sister chromatid cohesion sister chromatid cohesion maintenance of sister chromatid cohesion maintenance of mitotic sister chromatid cohesion cellular developmental process oogenesis cell development cellular process involved in reproduction in multicellular organism developmental process involved in reproduction anatomical structure morphogenesis eggshell formation cell differentiation anatomical structure formation involved in morphogenesis germ cell development maintenance of meiotic sister chromatid cohesion meiotic sister chromatid cohesion regulated exocytosis transport regulation of reproductive process fertilization negative regulation of biological process vesicle-mediated transport negative regulation of fertilization secretion by cell cortical granule exocytosis single fertilization calcium-ion regulated exocytosis secretion egg activation cell activation export from cell regulation of fertilization establishment of localization exocytosis negative regulation of reproductive process prevention of polyspermy polarity specification of anterior/posterior axis specification of axis polarity anterior/posterior pattern specification pattern specification process regionalization anterior/posterior axis specification axis specification regulation of localization regulation of cellular process regulation of transport regulation of vesicle-mediated transport regulation of secretion regulation of secretion by cell regulation of exocytosis microtubule organizing center organization regulation of cell cycle process microtubule cytoskeleton organization centriole-centriole cohesion centrosome cycle microtubule-based process regulation of cell cycle regulation of centriole-centriole cohesion cytoskeleton organization regulation of locomotion locomotion	midbody cellular anatomical entity cell periphery cell cortex intracellular anatomical structure cytoplasm membrane-bounded organelle intracellular organelle organelle envelope organelle nuclear envelope nucleus intracellular membrane-bounded organelle endomembrane system midbody cellular anatomical entity cell periphery cell cortex intracellular anatomical structure cytoplasm membrane-bounded organelle intracellular organelle organelle envelope organelle nuclear envelope nucleus intracellular membrane-bounded organelle endomembrane system condensed nuclear chromosome membrane-enclosed lumen nuclear chromosome chromosome intracellular organelle lumen organelle lumen intracellular non-membrane-bounded organelle condensed chromosome non-membrane-bounded organelle nuclear lumen centrosome microtubule organizing center cytoskeleton microtubule cytoskeleton intracellular vesicle cytoplasmic vesicle secretory granule secretory vesicle vesicle cortical granule protein-containing complex catalytic complex peptidase complex endopeptidase complex separase-securin complex mitotic spindle spindle cell division site cleavage furrow membrane plasma membrane plasma membrane region metaphase plate meiotic spindle
B	Interactor of FizzY protein	protein binding binding enzyme binding protease binding ubiquitin protein ligase binding ubiquitin-like protein ligase binding	maintenance of meiotic sister chromatid cohesion chromosome organization sister chromatid cohesion maintenance of sister chromatid cohesion cellular component organization organelle organization cellular process cellular component organization or biogenesis cell cycle cell cycle process meiotic sister chromatid cohesion regulation of protein stability biological regulation regulation of biological quality maintenance of meiotic sister chromatid cohesion chromosome organization sister chromatid cohesion maintenance of sister chromatid cohesion cellular component organization organelle organization cellular process cellular component organization or biogenesis cell cycle cell cycle process meiotic sister chromatid cohesion regulation of protein stability biological regulation regulation of biological quality protein stabilization mitotic cell cycle process cytoskeleton-dependent cytokinesis mitotic cytokinesis cytokinesis cell division mitotic cell cycle mitotic nuclear division organelle fission sister chromatid segregation nuclear division chromosome separation mitotic sister chromatid segregation nuclear chromosome segregation mitotic sister chromatid separation chromosome segregation multicellular organismal process regulated exocytosis sexual reproduction transport regulation of reproductive process reproductive process fertilization negative regulation of biological process vesicle-mediated transport negative regulation of fertilization localization secretion by cell cortical granule exocytosis single fertilization calcium-ion regulated exocytosis regulation of biological process secretion egg activation cell activation export from cell regulation of fertilization establishment of localization exocytosis negative regulation of reproductive process prevention of polyspermy meiotic cell cycle multicellular organismal reproductive process female gamete generation meiotic cell cycle process meiotic cytokinesis gamete generation female meiotic nuclear division polar body extrusion after meiotic divisions meiotic nuclear division centrosome localization microtubule organizing center localization meiotic chromosome segregation meiotic chromosome separation microtubule organizing center organization microtubule cytoskeleton organization centrosome duplication centrosome cycle microtubule-based process cytoskeleton organization mitotic sister chromatid cohesion maintenance of mitotic sister chromatid cohesion	intracellular organelle intracellular anatomical structure organelle intracellular non-membrane-bounded organelle chromosome cellular anatomical entity condensed chromosome non-membrane-bounded organelle protein-containing complex catalytic complex peptidase complex endopeptidase complex separase-securin complex polymeric cytoskeletal fiber intracellular organelle intracellular anatomical structure organelle intracellular non-membrane-bounded organelle chromosome cellular anatomical entity condensed chromosome non-membrane-bounded organelle protein-containing complex catalytic complex peptidase complex endopeptidase complex separase-securin complex polymeric cytoskeletal fiber spindle spindle microtubule cytoskeleton supramolecular fiber microtubule supramolecular polymer supramolecular complex microtubule cytoskeleton cytoplasm

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth 1]	IPR030397	SEPARIN core domain	Domain
A [auth 1]	IPR005314	Peptidase C50, separase	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.