5MUX

Crystal structure of 2-methylcitrate dehydratase (MmgE) from Bacillus subtilis.

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B	d5muxb_	Multi-domain proteins (alpha and beta)	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	automated matches	(Bacillus subtilis subsp. subtilis str. 168 ) [TaxId: 224308 ],	SCOPe (2.08)
C	d5muxc_	Multi-domain proteins (alpha and beta)	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	automated matches	(Bacillus subtilis subsp. subtilis str. 168 ) [TaxId: 224308 ],	SCOPe (2.08)
D	d5muxd_	Multi-domain proteins (alpha and beta)	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	automated matches	(Bacillus subtilis subsp. subtilis str. 168 ) [TaxId: 224308 ],	SCOPe (2.08)
F	d5muxf_	Multi-domain proteins (alpha and beta)	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	automated matches	(Bacillus subtilis subsp. subtilis str. 168 ) [TaxId: 224308 ],	SCOPe (2.08)
A	d5muxa_	Multi-domain proteins (alpha and beta)	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	automated matches	(Bacillus subtilis subsp. subtilis str. 168 ) [TaxId: 224308 ],	SCOPe (2.08)
E	d5muxe_	Multi-domain proteins (alpha and beta)	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	automated matches	(Bacillus subtilis subsp. subtilis str. 168 ) [TaxId: 224308 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	PF03972	e5muxB1	A: alpha arrays	X: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	H: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	T: alpha-helical domain in 2-methylcitrate dehydratase PrpD	F: PF03972	ECOD (1.6)
B	PF19305	e5muxB2	A: a+b two layers	X: FwdE/GAPDH domain-like	H: Serine dehydratase beta chain-like (From Topology)	T: Serine dehydratase beta chain-like	F: PF19305	ECOD (1.6)
C	PF03972	e5muxC2	A: alpha arrays	X: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	H: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	T: alpha-helical domain in 2-methylcitrate dehydratase PrpD	F: PF03972	ECOD (1.6)
C	PF19305	e5muxC1	A: a+b two layers	X: FwdE/GAPDH domain-like	H: Serine dehydratase beta chain-like (From Topology)	T: Serine dehydratase beta chain-like	F: PF19305	ECOD (1.6)
D	PF03972	e5muxD2	A: alpha arrays	X: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	H: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	T: alpha-helical domain in 2-methylcitrate dehydratase PrpD	F: PF03972	ECOD (1.6)
D	PF19305	e5muxD1	A: a+b two layers	X: FwdE/GAPDH domain-like	H: Serine dehydratase beta chain-like (From Topology)	T: Serine dehydratase beta chain-like	F: PF19305	ECOD (1.6)
F	PF03972	e5muxF1	A: alpha arrays	X: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	H: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	T: alpha-helical domain in 2-methylcitrate dehydratase PrpD	F: PF03972	ECOD (1.6)
F	PF19305	e5muxF2	A: a+b two layers	X: FwdE/GAPDH domain-like	H: Serine dehydratase beta chain-like (From Topology)	T: Serine dehydratase beta chain-like	F: PF19305	ECOD (1.6)
A	PF03972	e5muxA1	A: alpha arrays	X: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	H: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	T: alpha-helical domain in 2-methylcitrate dehydratase PrpD	F: PF03972	ECOD (1.6)
A	PF19305	e5muxA2	A: a+b two layers	X: FwdE/GAPDH domain-like	H: Serine dehydratase beta chain-like (From Topology)	T: Serine dehydratase beta chain-like	F: PF19305	ECOD (1.6)
E	PF03972	e5muxE1	A: alpha arrays	X: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	H: alpha-helical domain in 2-methylcitrate dehydratase PrpD (From Topology)	T: alpha-helical domain in 2-methylcitrate dehydratase PrpD	F: PF03972	ECOD (1.6)
E	PF19305	e5muxE2	A: a+b two layers	X: FwdE/GAPDH domain-like	H: Serine dehydratase beta chain-like (From Topology)	T: Serine dehydratase beta chain-like	F: PF19305	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	1.10.4100.10	Mainly Alpha	Orthogonal Bundle	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	CATH (4.3.0)
B	3.30.1330.120	Alpha Beta	2-Layer Sandwich	60s Ribosomal Protein L30	Chain: A	CATH (4.3.0)
C	1.10.4100.10	Mainly Alpha	Orthogonal Bundle	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	CATH (4.3.0)
C	3.30.1330.120	Alpha Beta	2-Layer Sandwich	60s Ribosomal Protein L30	Chain: A	CATH (4.3.0)
D	1.10.4100.10	Mainly Alpha	Orthogonal Bundle	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	CATH (4.3.0)
D	3.30.1330.120	Alpha Beta	2-Layer Sandwich	60s Ribosomal Protein L30	Chain: A	CATH (4.3.0)
F	1.10.4100.10	Mainly Alpha	Orthogonal Bundle	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	CATH (4.3.0)
F	3.30.1330.120	Alpha Beta	2-Layer Sandwich	60s Ribosomal Protein L30	Chain: A	CATH (4.3.0)
A	1.10.4100.10	Mainly Alpha	Orthogonal Bundle	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	CATH (4.3.0)
A	3.30.1330.120	Alpha Beta	2-Layer Sandwich	60s Ribosomal Protein L30	Chain: A	CATH (4.3.0)
E	1.10.4100.10	Mainly Alpha	Orthogonal Bundle	2-methylcitrate dehydratase PrpD	2-methylcitrate dehydratase PrpD	CATH (4.3.0)
E	3.30.1330.120	Alpha Beta	2-Layer Sandwich	60s Ribosomal Protein L30	Chain: A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F	PF19305	MmgE/PrpD C-terminal domain (MmgE_PrpD_C)	MmgE/PrpD C-terminal domain	This entry represents the C-terminal domain of 2-methylcitrate dehydratase EC:4.2.1.79 (PrpD) that is required for propionate catabolism. It catalyses the third step of the 2-methylcitric acid cycle. This domain is related to the serine dehydratase b ...	This entry represents the C-terminal domain of 2-methylcitrate dehydratase EC:4.2.1.79 (PrpD) that is required for propionate catabolism. It catalyses the third step of the 2-methylcitric acid cycle. This domain is related to the serine dehydratase beta chain.	Domain
A, B, C, D, E A, B, C, D, E, F	PF03972	MmgE/PrpD N-terminal domain (MmgE_PrpD_N)	MmgE/PrpD N-terminal domain	This entry represents the N-terminal domain of 2-methylcitrate dehydratase EC:4.2.1.79 (PrpD) that is required for propionate catabolism. It catalyses the third step of the 2-methylcitric acid cycle [1]. This enzyme consists of two domains: a large d ...	This entry represents the N-terminal domain of 2-methylcitrate dehydratase EC:4.2.1.79 (PrpD) that is required for propionate catabolism. It catalyses the third step of the 2-methylcitric acid cycle [1]. This enzyme consists of two domains: a large domain with an all-helical fold and a smaller domain that folds into an alpha/beta domain [2]. Cis-aconitic acid decarboxylase (CAD) shares high identity with proteins of the MmgE/PrpD family [3,4]. Citrate/2-methylcitrate dehydratase from Bacillus subtilis is involved in the tricarboxylic acid (TCA) and methylcitric acid cycles as it has both 2-methylcitrate dehydratase and citrate dehydratase activities [5]. This entry represents the N-terminal domain of 2-methylcitrate dehydratase PrpD, which has an all-helical fold.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F	2-methylcitrate dehydratase	iron-sulfur cluster binding 2 iron, 2 sulfur cluster binding binding metal cluster binding small molecule binding hydro-lyase activity lyase activity catalytic activity carbon-oxygen lyase activity 2-methylcitrate dehydratase activity	aerobic respiration cellular respiration tricarboxylic acid cycle energy derivation by oxidation of organic compounds cellular metabolic process metabolic process cellular process primary metabolic process generation of precursor metabolites and energy fatty acid metabolic process oxoacid metabolic process short-chain fatty acid metabolic process carboxylic acid metabolic process organic acid metabolic process aerobic respiration cellular respiration tricarboxylic acid cycle energy derivation by oxidation of organic compounds cellular metabolic process metabolic process cellular process primary metabolic process generation of precursor metabolites and energy fatty acid metabolic process oxoacid metabolic process short-chain fatty acid metabolic process carboxylic acid metabolic process organic acid metabolic process organic substance metabolic process cellular lipid metabolic process small molecule metabolic process propionate metabolic process, methylcitrate cycle monocarboxylic acid metabolic process propionate metabolic process lipid metabolic process sporulation resulting in formation of a cellular spore developmental process anatomical structure morphogenesis anatomical structure formation involved in morphogenesis anatomical structure development sporulation	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F	IPR005656	MmgE/PrpD	Family
A, B, C, D, E A, B, C, D, E, F	IPR036148	MmgE/PrpD superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR042188	MmgE/PrpD superfamily, domain 2	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR042183	MmgE/PrpD superfamily, domain 1	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR045337	MmgE/PrpD, C-terminal	Domain
A, B, C, D, E A, B, C, D, E, F	IPR012705	2-methylcitrate dehydratase PrpD	Family
A, B, C, D, E A, B, C, D, E, F	IPR045336	MmgE/PrpD, N-terminal	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.