5LU1

Human 14-3-3 sigma CLU3 mutant complexed with short HSPB6 phosphopeptide

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Protein Modification Annotation

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	14-3-3 protein	8082819	3001329	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	14-3-3 protein	8082819	3001329	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	14-3-3 protein	8082819	3001329	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	14-3-3 protein	8082819	3001329	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00244	e5lu1A1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: PF00244	ECOD (1.6)
B	PF00244	e5lu1B1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: PF00244	ECOD (1.6)
E	PF00244	e5lu1E1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: PF00244	ECOD (1.6)
F	PF00244	e5lu1F1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: PF00244	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.20.190.20	Mainly Alpha	Up-down Bundle	Delta-Endotoxin	domain 1	CATH (4.3.0)
B	1.20.190.20	Mainly Alpha	Up-down Bundle	Delta-Endotoxin	domain 1	CATH (4.3.0)
E	1.20.190.20	Mainly Alpha	Up-down Bundle	Delta-Endotoxin	domain 1	CATH (4.3.0)
F	1.20.190.20	Mainly Alpha	Up-down Bundle	Delta-Endotoxin	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, E, F	PF00244	14-3-3 protein (14-3-3)	14-3-3 protein	-	Repeat
C, D, G, H	PF00525	Alpha crystallin A chain, N terminal (Crystallin)	Alpha crystallin A chain, N terminal	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, E, F	14-3-3 protein sigma	phosphoprotein binding protein binding binding phosphoserine residue binding protein phosphorylated amino acid binding enzyme regulator activity protein kinase inhibitor activity molecular function inhibitor activity molecular function regulator activity enzyme inhibitor activity protein kinase C inhibitor activity protein kinase regulator activity protein serine/threonine kinase inhibitor activity kinase inhibitor activity phosphoprotein binding protein binding binding phosphoserine residue binding protein phosphorylated amino acid binding enzyme regulator activity protein kinase inhibitor activity molecular function inhibitor activity molecular function regulator activity enzyme inhibitor activity protein kinase C inhibitor activity protein kinase regulator activity protein serine/threonine kinase inhibitor activity kinase inhibitor activity kinase regulator activity molecular sequestering activity protein sequestering activity kinase binding enzyme binding protein kinase binding cell adhesion molecule binding cadherin binding identical protein binding	regulation of biological process regulation of growth regulation of cellular process regulation of cellular component organization positive regulation of biological process biological regulation growth positive regulation of cell growth regulation of cell growth cell growth cellular component organization positive regulation of growth cellular process cellular component organization or biogenesis regulation of biological process regulation of growth regulation of cellular process regulation of cellular component organization positive regulation of biological process biological regulation growth positive regulation of cell growth regulation of cell growth cell growth cellular component organization positive regulation of growth cellular process cellular component organization or biogenesis positive regulation of cellular process negative regulation of hydrolase activity negative regulation of proteolysis regulation of catalytic activity negative regulation of biological process negative regulation of catalytic activity regulation of programmed cell death negative regulation of endopeptidase activity regulation of primary metabolic process negative regulation of peptidase activity regulation of cysteine-type endopeptidase activity involved in apoptotic process organic substance metabolic process regulation of proteolysis proteolysis regulation of endopeptidase activity negative regulation of cellular process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process protein metabolic process negative regulation of protein metabolic process negative regulation of macromolecule metabolic process regulation of cysteine-type endopeptidase activity organonitrogen compound metabolic process negative regulation of programmed cell death regulation of protein metabolic process regulation of apoptotic process negative regulation of metabolic process regulation of peptidase activity negative regulation of molecular function primary metabolic process regulation of macromolecule metabolic process cell death regulation of molecular function negative regulation of apoptotic process regulation of hydrolase activity negative regulation of cysteine-type endopeptidase activity metabolic process macromolecule metabolic process apoptotic process regulation of metabolic process programmed cell death protein localization cellular localization establishment of protein localization nitrogen compound transport nucleocytoplasmic transport transport nuclear transport organic substance transport intracellular transport protein export from nucleus establishment of localization localization protein transport establishment of localization in cell cellular macromolecule localization macromolecule localization nuclear export intracellular protein transport epidermal cell division cell division regulation of epidermal cell division regulation of cell division developmental process establishment of skin barrier epithelium development skin development skin epidermis development epidermis development anatomical structure development animal organ development tissue development regulation of defense response regulation of response to biotic stimulus biological process involved in interspecies interaction between organisms negative regulation of response to biotic stimulus response to stimulus regulation of response to external stimulus response to biotic stimulus response to stress regulation of response to stress innate immune response regulation of immune system process response to external biotic stimulus negative regulation of response to stimulus immune response response to other organism defense response regulation of innate immune response negative regulation of immune response defense response to symbiont response to external stimulus regulation of response to stimulus negative regulation of immune system process immune system process defense response to other organism negative regulation of response to external stimulus negative regulation of defense response regulation of immune response negative regulation of innate immune response cell population proliferation epithelial cell proliferation keratinocyte proliferation cellular response to stimulus signaling intracellular signal transduction cell communication protein kinase A signaling signal transduction negative regulation of cell population proliferation regulation of stem cell proliferation negative regulation of stem cell proliferation stem cell proliferation regulation of cell population proliferation regulation of transferase activity negative regulation of cellular metabolic process negative regulation of kinase activity negative regulation of transferase activity regulation of phosphate metabolic process phosphate-containing compound metabolic process negative regulation of protein modification process phosphorus metabolic process protein modification process protein phosphorylation phosphorylation cellular metabolic process regulation of cellular metabolic process regulation of kinase activity negative regulation of protein phosphorylation macromolecule modification regulation of phosphorus metabolic process regulation of protein modification process regulation of protein phosphorylation negative regulation of phosphate metabolic process negative regulation of protein kinase activity regulation of protein kinase activity negative regulation of phosphorus metabolic process regulation of phosphorylation negative regulation of phosphorylation regulation of localization regulation of protein localization positive regulation of nucleocytoplasmic transport positive regulation of establishment of protein localization positive regulation of protein transport positive regulation of intracellular transport regulation of protein export from nucleus regulation of intracellular transport regulation of transport regulation of establishment of protein localization regulation of nucleocytoplasmic transport regulation of protein transport positive regulation of protein export from nucleus positive regulation of transport regulation of cellular localization positive regulation of protein localization regulation of intracellular protein transport positive regulation of intracellular protein transport apoptotic signaling pathway cellular response to stress DNA damage response intrinsic apoptotic signaling pathway in response to DNA damage intrinsic apoptotic signaling pathway multicellular organismal process keratinization epidermal cell differentiation cellular developmental process epithelial cell differentiation cell differentiation keratinocyte differentiation keratinocyte development cell development epithelial cell development regulation of keratinocyte proliferation negative regulation of keratinocyte proliferation regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation cell cycle regulation of cell cycle negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process transcription by RNA polymerase II organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process regulation of transcription by RNA polymerase II negative regulation of DNA-templated transcription negative regulation of transcription by RNA polymerase II negative regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process organic cyclic compound biosynthetic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription negative regulation of RNA metabolic process cellular biosynthetic process RNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process regulation of RNA biosynthetic process regulation of biosynthetic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process negative regulation of biosynthetic process apoptotic mitochondrial changes release of cytochrome c from mitochondria organelle organization mitochondrion organization regulation of developmental process regulation of epidermal cell differentiation regulation of cell differentiation regulation of epithelial cell differentiation positive regulation of developmental process multicellular organism development positive regulation of cell differentiation regulation of multicellular organismal process regulation of multicellular organismal development positive regulation of epidermis development positive regulation of epithelial cell differentiation regulation of epidermis development positive regulation of epidermal cell differentiation	extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space cellular anatomical entity vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol intracellular organelle extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space cellular anatomical entity vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol intracellular organelle nucleus intracellular membrane-bounded organelle
C, D, G, H	Heat shock protein beta-6	unfolded protein binding protein binding binding identical protein binding protein homodimerization activity protein dimerization activity structural molecule activity structural constituent of eye lens protein folding chaperone protein-folding chaperone binding	protein refolding organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process protein folding biosynthetic process organic substance biosynthetic process metabolic process cellular process protein refolding organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process protein folding biosynthetic process organic substance biosynthetic process metabolic process cellular process protein metabolic process macromolecule metabolic process multicellular organismal process regulation of developmental process positive regulation of biological process angiogenesis regulation of vasculature development positive regulation of developmental process multicellular organism development tube morphogenesis anatomical structure development regulation of multicellular organismal process regulation of anatomical structure morphogenesis blood vessel development system development developmental process regulation of multicellular organismal development regulation of biological process blood vessel morphogenesis tube development biological regulation positive regulation of angiogenesis vasculature development circulatory system development anatomical structure morphogenesis positive regulation of vasculature development regulation of angiogenesis anatomical structure formation involved in morphogenesis positive regulation of multicellular organismal process regulation of cellular process negative regulation of programmed cell death regulation of apoptotic process negative regulation of biological process regulation of programmed cell death cell death negative regulation of apoptotic process negative regulation of cellular process apoptotic process programmed cell death chaperone-mediated protein folding response to stimulus response to stress response to heat response to abiotic stimulus response to temperature stimulus	membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle nucleus membrane-enclosed lumen intracellular organelle lumen nuclear speck nuclear body nucleoplasm membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle nucleus membrane-enclosed lumen intracellular organelle lumen nuclear speck nuclear body nucleoplasm organelle lumen nuclear lumen extracellular region cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, E, F	IPR023409	14-3-3 protein, conserved site	Conserved Site
A, B, E, F	IPR023410	14-3-3 domain	Domain
A, B, E, F	IPR037435	14-3-3 protein sigma	Family
A, B, E, F	IPR036815	14-3-3 domain superfamily	Homologous Superfamily
A, B, E, F	IPR000308	14-3-3 protein	Family
C, D, G, H	IPR002068	Alpha crystallin/Hsp20 domain	Domain
C, D, G, H	IPR001436	Alpha crystallin/Small heat shock protein, animal type	Family
C, D, G, H	IPR003090	Alpha-crystallin, N-terminal	Domain
C, D, G, H	IPR008978	HSP20-like chaperone	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, E, F	Pharos: P31947	esophageal squamous cell carcinoma squamous cell carcinoma esophageal cancer Lymphatic Metastasis keloid lung adenocarcinoma malignant pancreatic neoplasm non-small cell lung carcinoma ovarian cancer pancreatic intraductal papillary-mucinous neoplasm sarcoidosis cystic fibrosis interstitial cystitis malignant mesothelioma oral cavity neoplasm esophageal squamous cell carcinoma squamous cell carcinoma esophageal cancer Lymphatic Metastasis keloid lung adenocarcinoma malignant pancreatic neoplasm non-small cell lung carcinoma ovarian cancer pancreatic intraductal papillary-mucinous neoplasm sarcoidosis cystic fibrosis interstitial cystitis malignant mesothelioma oral cavity neoplasm pancreatic ductal adenocarcinoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia atypical teratoid rhabdoid tumor spina bifida diabetes mellitus malignant ependymoma neoplasm of esophagus osteosarcoma pancreatic intraductal papillary-mucinous carcinoma psoriasis breast cancer ductal breast carcinoma in situ hepatocellular carcinoma large cell medulloblastoma lung cancer malignant glioma
C, D, G, H	Pharos: O14558	heart disease non-small cell lung carcinoma polycystic ovary syndrome Adverse reaction to drug dermatomyositis ductal breast carcinoma in situ lung cancer malignant mesothelioma myopathy ovarian cancer psoriasis atypical teratoid rhabdoid tumor Gaucher disease invasive ductal breast carcinoma malignant colon neoplasm heart disease non-small cell lung carcinoma polycystic ovary syndrome Adverse reaction to drug dermatomyositis ductal breast carcinoma in situ lung cancer malignant mesothelioma myopathy ovarian cancer psoriasis atypical teratoid rhabdoid tumor Gaucher disease invasive ductal breast carcinoma malignant colon neoplasm malignant pancreatic neoplasm nephrotic syndrome pancreatic ductal adenocarcinoma breast carcinoma glioblastoma large cell medulloblastoma malignant glioma medulloblastoma

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
C, D, G, H	SEP	Parent Component: SER RESID: AA0037 PSI-MOD : O-phospho-L-serine MOD:00046

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.