5KQ1

Crystal structure of S. pombe Dcp1/Dcp2 in complex with H. sapiens PNRC2

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
C [auth B]	d5kq1b1	All alpha proteins	Dcp2 domain-like	Dcp2 domain-like	automated matches	automated matches	Fission yeast (Schizosaccharomyces pombe 972h- ) [TaxId: 284812 ],	SCOPe (2.08)
C [auth B]	d5kq1b2	Alpha and beta proteins (a+b)	Nudix	Nudix	mRNA decapping enzyme-like	automated matches	Fission yeast (Schizosaccharomyces pombe 972h- ) [TaxId: 284812 ],	SCOPe (2.08)
C [auth B]	d5kq1b3	Artifacts	Tags	Tags	Tags	N-terminal Tags	Fission yeast (Schizosaccharomyces pombe 972h- ) [TaxId: 284812 ],	SCOPe (2.08)
F [auth E]	d5kq1e1	All alpha proteins	Dcp2 domain-like	Dcp2 domain-like	automated matches	automated matches	Fission yeast (Schizosaccharomyces pombe 972h- ) [TaxId: 284812 ],	SCOPe (2.08)
F [auth E]	d5kq1e2	Alpha and beta proteins (a+b)	Nudix	Nudix	mRNA decapping enzyme-like	automated matches	Fission yeast (Schizosaccharomyces pombe 972h- ) [TaxId: 284812 ],	SCOPe (2.08)
F [auth E]	d5kq1e3	Artifacts	Tags	Tags	Tags	N-terminal Tags	Fission yeast (Schizosaccharomyces pombe 972h- ) [TaxId: 284812 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
C [auth B]	SCOP2B Superfamily	Nudix	8036684	3000098	SCOP2B (2022-06-29)
C [auth B]	SCOP2B Superfamily	Dcp2 domain-like	8036072	3001348	SCOP2B (2022-06-29)
F [auth E]	SCOP2B Superfamily	Nudix	8036684	3000098	SCOP2B (2022-06-29)
F [auth E]	SCOP2B Superfamily	Dcp2 domain-like	8036072	3001348	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF06058	e5kq1A1	A: beta barrels	X: PH domain-like	H: PH domain-like (From Topology)	T: PH domain-like	F: PF06058	ECOD (1.6)
D	PF06058	e5kq1D1	A: beta barrels	X: PH domain-like	H: PH domain-like (From Topology)	T: PH domain-like	F: PF06058	ECOD (1.6)
C [auth B]	PF05026	e5kq1B2	A: alpha arrays	X: Dcp2 box A domain (From Topology)	H: Dcp2 box A domain (From Topology)	T: Dcp2 box A domain	F: PF05026	ECOD (1.6)
C [auth B]	PF00293	e5kq1B1	A: a+b two layers	X: beta-Grasp	H: Nudix (From Topology)	T: Nudix	F: PF00293	ECOD (1.6)
F [auth E]	PF05026	e5kq1E2	A: alpha arrays	X: Dcp2 box A domain (From Topology)	H: Dcp2 box A domain (From Topology)	T: Dcp2 box A domain	F: PF05026	ECOD (1.6)
F [auth E]	PF00293	e5kq1E1	A: a+b two layers	X: beta-Grasp	H: Nudix (From Topology)	T: Nudix	F: PF00293	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.30.29.30	Mainly Beta	Roll	PH-domain like	Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)	CATH (4.3.0)
D	2.30.29.30	Mainly Beta	Roll	PH-domain like	Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, D	PF06058	Dcp1-like decapping family (DCP1)	Dcp1-like decapping family	An essential step in mRNA turnover is decapping. In yeast, two proteins have been identified that are essential for decapping, Dcp1 (this family) and Dcp2 (Pfam:PF05026). The precise role of these proteins in the decapping reaction have not been es ...	An essential step in mRNA turnover is decapping. In yeast, two proteins have been identified that are essential for decapping, Dcp1 (this family) and Dcp2 (Pfam:PF05026). The precise role of these proteins in the decapping reaction have not been established. Evidence suggests that the Dcp1 may enhance the function of Dcp2 [1].	Domain
B [auth C], E [auth F]	PF15365	Proline-rich nuclear receptor coactivator motif (PNRC)	Proline-rich nuclear receptor coactivator motif	-	Motif
C [auth B], F [auth E]	PF00293	NUDIX domain (NUDIX)	NUDIX domain	-	Domain
C [auth B], F [auth E]	PF05026	Dcp2, box A domain (DCP2)	Dcp2, box A domain	This domain is always found to the amino terminal side of Pfam:PF00293. This domain is specific to mRNA decapping protein 2 and this region has been termed Box A [2]. Removal of the cap structure is catalysed by the Dcp1-Dcp2 complex [3].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, D	mRNA-decapping enzyme subunit 1	enzyme regulator activity enzyme activator activity mRNA phosphatase activator activity molecular function activator activity molecular function regulator activity RNA binding binding organic cyclic compound binding nucleic acid binding	regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process positive regulation of metabolic process post-transcriptional regulation of gene expression regulation of RNA metabolic process organic substance biosynthetic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process regulation of biological quality RNA catabolic process mRNA catabolic process regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process positive regulation of metabolic process post-transcriptional regulation of gene expression regulation of RNA metabolic process organic substance biosynthetic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process regulation of biological quality RNA catabolic process mRNA catabolic process regulation of biological process positive regulation of nucleobase-containing compound metabolic process cellular metabolic process negative regulation of metabolic process positive regulation of mRNA metabolic process primary metabolic process regulation of cellular metabolic process negative regulation of cellular biosynthetic process nucleobase-containing compound catabolic process positive regulation of RNA metabolic process organic cyclic compound metabolic process regulation of gene expression negative regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process nucleic acid catabolic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process deadenylation-dependent decapping of nuclear-transcribed mRNA regulation of metabolic process RNA decapping nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay catabolic process cellular biosynthetic process positive regulation of biological process RNA metabolic process macromolecule catabolic process mRNA destabilization organic substance metabolic process regulation of mRNA catabolic process negative regulation of cellular process mRNA methylguanosine-cap decapping negative regulation of macromolecule metabolic process regulation of biosynthetic process nuclear-transcribed mRNA catabolic process RNA destabilization biological regulation regulation of macromolecule metabolic process regulation of catabolic process gene expression mRNA metabolic process macromolecule biosynthetic process nucleic acid metabolic process organic cyclic compound catabolic process regulation of cellular biosynthetic process positive regulation of catabolic process regulation of mRNA stability negative regulation of biosynthetic process positive regulation of mRNA catabolic process regulation of RNA stability cellular process regulation of mRNA metabolic process organic substance catabolic process nuclear-transcribed mRNA catabolic process, nonsense-mediated decay mRNA processing RNA processing	intracellular anatomical structure cytoplasmic ribonucleoprotein granule cellular anatomical entity ribonucleoprotein granule intracellular organelle organelle intracellular non-membrane-bounded organelle cytoplasm supramolecular complex non-membrane-bounded organelle cytoplasmic stress granule cytosol protein-containing complex RNA decapping complex intracellular anatomical structure cytoplasmic ribonucleoprotein granule cellular anatomical entity ribonucleoprotein granule intracellular organelle organelle intracellular non-membrane-bounded organelle cytoplasm supramolecular complex non-membrane-bounded organelle cytoplasmic stress granule cytosol protein-containing complex RNA decapping complex P-body membrane-bounded organelle nucleus intracellular membrane-bounded organelle
B [auth C], E [auth F]	Proline-rich nuclear receptor coactivator 2	-	regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process nucleobase-containing compound metabolic process organic substance biosynthetic process RNA catabolic process nuclear-transcribed mRNA catabolic process, deadenylation-independent decay mRNA catabolic process regulation of biological process cellular metabolic process negative regulation of metabolic process primary metabolic process regulation of cellular metabolic process negative regulation of cellular biosynthetic process regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process nucleobase-containing compound metabolic process organic substance biosynthetic process RNA catabolic process nuclear-transcribed mRNA catabolic process, deadenylation-independent decay mRNA catabolic process regulation of biological process cellular metabolic process negative regulation of metabolic process primary metabolic process regulation of cellular metabolic process negative regulation of cellular biosynthetic process nucleobase-containing compound catabolic process organic cyclic compound metabolic process regulation of gene expression negative regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process nucleic acid catabolic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of metabolic process RNA decapping catabolic process cellular biosynthetic process RNA metabolic process macromolecule catabolic process organic substance metabolic process deadenylation-independent decapping of nuclear-transcribed mRNA negative regulation of cellular process mRNA methylguanosine-cap decapping negative regulation of macromolecule metabolic process regulation of biosynthetic process nuclear-transcribed mRNA catabolic process biological regulation regulation of macromolecule metabolic process gene expression mRNA metabolic process macromolecule biosynthetic process nucleic acid metabolic process organic cyclic compound catabolic process regulation of cellular biosynthetic process negative regulation of biosynthetic process cellular process organic substance catabolic process nuclear-transcribed mRNA catabolic process, nonsense-mediated decay	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol membrane-bounded organelle intracellular organelle Golgi apparatus organelle intracellular membrane-bounded organelle endomembrane system cytoplasmic ribonucleoprotein granule ribonucleoprotein granule intracellular non-membrane-bounded organelle supramolecular complex intracellular anatomical structure cytoplasm cellular anatomical entity cytosol membrane-bounded organelle intracellular organelle Golgi apparatus organelle intracellular membrane-bounded organelle endomembrane system cytoplasmic ribonucleoprotein granule ribonucleoprotein granule intracellular non-membrane-bounded organelle supramolecular complex P-body non-membrane-bounded organelle nucleus membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen
C [auth B], F [auth E]	mRNA decapping complex subunit 2	RNA binding mRNA cap binding binding organic cyclic compound binding nucleic acid binding RNA cap binding mRNA binding cation binding manganese ion binding transition metal ion binding ion binding metal ion binding small molecule binding single-stranded RNA binding RNA binding mRNA cap binding binding organic cyclic compound binding nucleic acid binding RNA cap binding mRNA binding cation binding manganese ion binding transition metal ion binding ion binding metal ion binding small molecule binding single-stranded RNA binding magnesium ion binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding pyrophosphatase activity 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity 5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity	regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process positive regulation of metabolic process post-transcriptional regulation of gene expression regulation of RNA metabolic process organic substance biosynthetic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process regulation of biological quality RNA catabolic process mRNA catabolic process regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process positive regulation of metabolic process post-transcriptional regulation of gene expression regulation of RNA metabolic process organic substance biosynthetic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process regulation of biological quality RNA catabolic process mRNA catabolic process regulation of biological process positive regulation of nucleobase-containing compound metabolic process cellular metabolic process negative regulation of metabolic process positive regulation of mRNA metabolic process primary metabolic process regulation of cellular metabolic process negative regulation of cellular biosynthetic process nucleobase-containing compound catabolic process positive regulation of RNA metabolic process organic cyclic compound metabolic process regulation of gene expression negative regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process nucleic acid catabolic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process deadenylation-dependent decapping of nuclear-transcribed mRNA regulation of metabolic process RNA decapping nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay catabolic process cellular biosynthetic process positive regulation of biological process RNA metabolic process macromolecule catabolic process mRNA destabilization organic substance metabolic process regulation of mRNA catabolic process negative regulation of cellular process mRNA methylguanosine-cap decapping negative regulation of macromolecule metabolic process regulation of biosynthetic process nuclear-transcribed mRNA catabolic process RNA destabilization biological regulation regulation of macromolecule metabolic process regulation of catabolic process gene expression mRNA metabolic process macromolecule biosynthetic process nucleic acid metabolic process organic cyclic compound catabolic process regulation of cellular biosynthetic process positive regulation of catabolic process regulation of mRNA stability negative regulation of biosynthetic process positive regulation of mRNA catabolic process regulation of RNA stability cellular process regulation of mRNA metabolic process organic substance catabolic process mRNA processing RNA processing nuclear-transcribed mRNA catabolic process, nonsense-mediated decay	intracellular anatomical structure cytoplasmic ribonucleoprotein granule cellular anatomical entity ribonucleoprotein granule intracellular organelle organelle intracellular non-membrane-bounded organelle cytoplasm supramolecular complex non-membrane-bounded organelle cytoplasmic stress granule cytosol protein-containing complex RNA decapping complex intracellular anatomical structure cytoplasmic ribonucleoprotein granule cellular anatomical entity ribonucleoprotein granule intracellular organelle organelle intracellular non-membrane-bounded organelle cytoplasm supramolecular complex non-membrane-bounded organelle cytoplasmic stress granule cytosol protein-containing complex RNA decapping complex P-body membrane-bounded organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, D	IPR010334	mRNA-decapping enzyme subunit 1	Family
A, D	IPR011993	PH-like domain superfamily	Homologous Superfamily
B [auth C], E [auth F]	IPR026780	Proline-rich nuclear receptor coactivator 1/2	Family
C [auth B], F [auth E]	IPR015797	NUDIX hydrolase-like domain superfamily	Homologous Superfamily
C [auth B], F [auth E]	IPR036189	mRNA decapping protein 2, Box A domain superfamily	Homologous Superfamily
C [auth B], F [auth E]	IPR007722	mRNA decapping protein 2, Box A domain	Domain
C [auth B], F [auth E]	IPR044099	mRNA decapping enzyme 2 , NUDIX hydrolase domain	Domain
C [auth B], F [auth E]	IPR000086	NUDIX hydrolase domain	Domain
C [auth B], F [auth E]	IPR020084	NUDIX hydrolase, conserved site	Conserved Site

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
B [auth C], E [auth F]	Pharos: Q9NPJ4	adenoma myopathy ovarian cancer polycystic ovary syndrome dermatomyositis non-small cell lung carcinoma pancreatic intraductal papillary-mucinous carcinoma lymphoplasmacytic lymphoma osteosarcoma plasma cell myeloma

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.