5HDN

Crystal structure of heat shock factor1-DBD complex with ds-DNA and TtT

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d5hdna1	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	Heat-shock transcription factor	automated matches	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d5hdna2	Artifacts	Tags	Tags	Tags	N-terminal Tags	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d5hdnb1	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	Heat-shock transcription factor	automated matches	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d5hdnb2	Artifacts	Tags	Tags	Tags	N-terminal Tags	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
E [auth C]	d5hdnc1	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	Heat-shock transcription factor	automated matches	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
E [auth C]	d5hdnc2	Artifacts	Tags	Tags	Tags	N-terminal Tags	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
F [auth D]	d5hdnd1	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	Heat-shock transcription factor	automated matches	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
F [auth D]	d5hdnd2	Artifacts	Tags	Tags	Tags	N-terminal Tags	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Winged helix DNA-binding domain	8078533	3000034	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Winged helix DNA-binding domain	8078533	3000034	SCOP2B (2022-06-29)
E [auth C]	SCOP2B Superfamily	Winged helix DNA-binding domain	8078533	3000034	SCOP2B (2022-06-29)
F [auth D]	SCOP2B Superfamily	Winged helix DNA-binding domain	8078533	3000034	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00447	e5hdnA1	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF00447	ECOD (1.6)
B	PF00447	e5hdnB1	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF00447	ECOD (1.6)
E [auth C]	PF00447	e5hdnC1	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF00447	ECOD (1.6)
F [auth D]	PF00447	e5hdnD1	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF00447	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
B	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
E [auth C]	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
F [auth D]	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, E [auth C], F [auth D]	PF00447	HSF-type DNA-binding (HSF_DNA-bind)	HSF-type DNA-binding	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, E [auth C], F [auth D]	Heat shock factor protein 1	DNA binding sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding binding double-stranded DNA binding organic cyclic compound binding nucleic acid binding transcription regulatory region nucleic acid binding transcription factor binding RNA polymerase II-specific DNA-binding transcription factor binding protein binding DNA-binding transcription factor binding STAT family protein binding DNA binding sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding binding double-stranded DNA binding organic cyclic compound binding nucleic acid binding transcription regulatory region nucleic acid binding transcription factor binding RNA polymerase II-specific DNA-binding transcription factor binding protein binding DNA-binding transcription factor binding STAT family protein binding heat shock protein binding protein-containing complex binding chromatin binding promoter-specific chromatin binding DNA-binding transcription factor activity, RNA polymerase II-specific transcription regulator activity DNA-binding transcription factor activity DNA-binding transcription activator activity, RNA polymerase II-specific DNA-binding transcription activator activity identical protein binding general transcription initiation factor binding DNA-binding transcription repressor activity, RNA polymerase II-specific DNA-binding transcription repressor activity Hsp90 protein binding RNA polymerase II transcription regulatory region sequence-specific DNA binding cis-regulatory region sequence-specific DNA binding RNA polymerase II cis-regulatory region sequence-specific DNA binding protein heterodimerization activity protein dimerization activity kinase binding enzyme binding protein kinase binding chromatin DNA binding RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding intronic transcription regulatory region sequence-specific DNA binding translation elongation factor binding sequence-specific single stranded DNA binding single-stranded DNA binding	regulation of biological process regulation of cellular process regulation of cellular component organization negative regulation of cellular component organization regulation of inclusion body assembly biological regulation negative regulation of biological process inclusion body assembly regulation of cellular component biogenesis cellular component biogenesis cellular component organization cellular component assembly negative regulation of cellular process cellular process regulation of biological process regulation of cellular process regulation of cellular component organization negative regulation of cellular component organization regulation of inclusion body assembly biological regulation negative regulation of biological process inclusion body assembly regulation of cellular component biogenesis cellular component biogenesis cellular component organization cellular component assembly negative regulation of cellular process cellular process cellular component organization or biogenesis negative regulation of inclusion body assembly cellular response to nitrogen compound cellular response to stimulus response to organic substance response to stimulus response to oxygen-containing compound response to nitrogen compound response to nitroglycerin cellular response to oxygen-containing compound cellular response to nitroglycerin cellular response to chemical stimulus response to chemical cellular response to arsenic-containing substance response to sodium arsenite response to arsenic-containing substance response to inorganic substance cellular response to salt cellular response to sodium arsenite response to salt cellular biosynthetic process positive regulation of biological process positive regulation of cellular metabolic process positive regulation of macromolecule biosynthetic process positive regulation of metabolic process organic substance metabolic process positive regulation of biosynthetic process organic substance biosynthetic process positive regulation of macromolecule metabolic process positive regulation of cellular process regulation of biosynthetic process cellular metabolic process regulation of cellular metabolic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process positive regulation of gene expression regulation of macromolecule biosynthetic process metabolic process macromolecule metabolic process regulation of metabolic process positive regulation of cellular biosynthetic process response to ketone response to lipid response to testosterone response to organic cyclic compound cellular response to heat cellular response to stress response to stress response to heat response to abiotic stimulus response to temperature stimulus cellular response to inorganic substance cellular response to metal ion response to potassium ion response to metal ion cellular response to potassium ion positive regulation of DNA-binding transcription factor activity RNA metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process positive regulation of molecular function nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process primary metabolic process nucleobase-containing compound biosynthetic process nucleic acid metabolic process regulation of molecular function organic cyclic compound metabolic process regulation of DNA-binding transcription factor activity organic cyclic compound biosynthetic process regulation of DNA-templated transcription positive regulation of cellular component organization positive regulation of cellular component biogenesis positive regulation of inclusion body assembly protein-containing complex organization negative regulation of protein-containing complex assembly regulation of protein-containing complex assembly protein-containing complex assembly mRNA processing mRNA metabolic process RNA processing defense response signaling intracellular signal transduction cell communication intracellular signaling cassette MAPK cascade signal transduction cellular response to acid chemical cellular response to amino acid stimulus cellular response to L-glutamine response to organonitrogen compound response to endogenous stimulus response to L-glutamine response to amino acid cellular response to endogenous stimulus response to acid chemical cellular response to organonitrogen compound cellular response to organic substance DNA metabolic process DNA damage response DNA repair negative regulation of cardiac muscle cell apoptotic process muscle cell apoptotic process negative regulation of programmed cell death regulation of apoptotic process regulation of cardiac muscle cell apoptotic process regulation of muscle cell apoptotic process regulation of programmed cell death cell death cardiac muscle cell apoptotic process negative regulation of apoptotic process regulation of striated muscle cell apoptotic process striated muscle cell apoptotic process negative regulation of muscle cell apoptotic process negative regulation of striated muscle cell apoptotic process apoptotic process programmed cell death multicellular organismal process myeloid leukocyte differentiation regulation of leukocyte differentiation cellular developmental process regulation of developmental process cell development regulation of cell differentiation mononuclear cell differentiation regulation of cell development positive regulation of developmental process multicellular organism development positive regulation of cell differentiation positive regulation of hemopoiesis cell differentiation anatomical structure development regulation of immune system process regulation of multicellular organismal process regulation of macrophage differentiation positive regulation of immune system process developmental process regulation of multicellular organismal development hemopoiesis regulation of myeloid cell differentiation positive regulation of cell development macrophage differentiation regulation of myeloid leukocyte differentiation positive regulation of myeloid leukocyte differentiation positive regulation of macrophage differentiation myeloid cell differentiation immune system process positive regulation of myeloid cell differentiation regulation of hemopoiesis positive regulation of multicellular organismal process positive regulation of leukocyte differentiation leukocyte differentiation cellular response to xenobiotic stimulus response to xenobiotic stimulus cellular response to topologically incorrect protein cellular response to unfolded protein response to unfolded protein response to topologically incorrect protein response to diamide cellular response to diamide negative regulation of cellular metabolic process negative regulation of RNA biosynthetic process transcription by RNA polymerase II negative regulation of nucleobase-containing compound metabolic process regulation of transcription by RNA polymerase II negative regulation of DNA-templated transcription negative regulation of transcription by RNA polymerase II negative regulation of metabolic process negative regulation of cellular biosynthetic process negative regulation of macromolecule biosynthetic process negative regulation of RNA metabolic process negative regulation of macromolecule metabolic process negative regulation of biosynthetic process response to cadmium ion cellular response to cadmium ion response to hypobaric hypoxia response to decreased oxygen levels response to hypoxia response to oxygen levels positive regulation of nucleobase-containing compound metabolic process positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process positive regulation of DNA-templated transcription positive regulation of RNA biosynthetic process regulation of cellular response to heat regulation of cellular response to stress regulation of response to stimulus regulation of response to stress RNA transport nitrogen compound transport transport organic substance transport nucleic acid transport mRNA transport establishment of localization localization establishment of RNA localization macromolecule localization RNA localization nucleobase-containing compound transport cellular response to ionizing radiation cellular response to radiation cellular response to environmental stimulus response to ionizing radiation cellular response to gamma radiation response to radiation cellular response to abiotic stimulus response to gamma radiation response to activity regulation of double-strand break repair negative regulation of double-strand break repair via nonhomologous end joining negative regulation of DNA repair negative regulation of double-strand break repair regulation of double-strand break repair via nonhomologous end joining double-strand break repair double-strand break repair via nonhomologous end joining negative regulation of response to stimulus regulation of DNA repair negative regulation of DNA metabolic process regulation of DNA metabolic process biological process involved in interspecies interaction between organisms response to other organism cellular response to lipopolysaccharide response to external stimulus cellular response to molecule of bacterial origin response to lipopolysaccharide response to biotic stimulus cellular response to biotic stimulus response to bacterium cellular response to lipid response to external biotic stimulus response to molecule of bacterial origin response to copper ion cellular response to copper ion response to psychosocial stress cellular response to oxidative stress cellular response to chemical stress cellular response to hydrogen peroxide response to reactive oxygen species response to hydrogen peroxide response to oxidative stress cellular response to reactive oxygen species catabolic process macromolecule catabolic process regulation of DNA catabolic process execution phase of apoptosis positive regulation of DNA catabolic process DNA catabolic process apoptotic nuclear changes regulation of apoptotic DNA fragmentation cellular component disassembly positive regulation of apoptotic DNA fragmentation regulation of catabolic process organic cyclic compound catabolic process nucleobase-containing compound catabolic process positive regulation of catabolic process apoptotic DNA fragmentation positive regulation of DNA metabolic process cellular component disassembly involved in execution phase of apoptosis nucleic acid catabolic process organic substance catabolic process homeostatic process adaptive thermogenesis multicellular organismal-level homeostasis regulation of cold-induced thermogenesis cold-induced thermogenesis temperature homeostasis positive regulation of cold-induced thermogenesis positive regulation of phosphorylation positive regulation of phosphate metabolic process regulation of phosphate metabolic process peptidyl-amino acid modification phosphate-containing compound metabolic process protein modification process phosphorus metabolic process positive regulation of protein phosphorylation tyrosine phosphorylation of STAT protein protein phosphorylation positive regulation of phosphorus metabolic process phosphorylation regulation of protein metabolic process macromolecule modification regulation of phosphorus metabolic process cell surface receptor signaling pathway via STAT regulation of protein modification process regulation of protein phosphorylation positive regulation of protein metabolic process peptidyl-tyrosine modification positive regulation of peptidyl-tyrosine phosphorylation positive regulation of protein modification process protein metabolic process organonitrogen compound metabolic process cell surface receptor signaling pathway regulation of tyrosine phosphorylation of STAT protein regulation of peptidyl-tyrosine phosphorylation positive regulation of tyrosine phosphorylation of STAT protein regulation of phosphorylation peptidyl-tyrosine phosphorylation cell surface receptor signaling pathway via JAK-STAT cellular response to organic cyclic compound response to estradiol cellular response to estradiol stimulus negative regulation of gene expression positive regulation of cell cycle mitotic cell cycle regulation of mitotic cell cycle positive regulation of mitotic cell cycle cell cycle regulation of cell cycle response to nutrient levels response to nutrient response to angiotensin response to peptide hormone cellular response to peptide hormone stimulus cellular response to angiotensin response to hormone cellular response to hormone stimulus	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol intracellular organelle protein-containing complex protein-DNA complex organelle intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle protein folding chaperone complex intracellular protein-containing complex intracellular anatomical structure cytoplasm cellular anatomical entity cytosol intracellular organelle protein-containing complex protein-DNA complex organelle intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle protein folding chaperone complex intracellular protein-containing complex heterochromatin perinuclear region of cytoplasm mitotic spindle spindle cytoskeleton mitotic spindle pole spindle pole microtubule cytoskeleton centrosome microtubule organizing center membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen chromosomal region kinetochore chromosome, centromeric region condensed chromosome, centromeric region supramolecular complex condensed chromosome nuclear stress granule ribonucleoprotein granule euchromatin ribonucleoprotein complex nuclear body PML body
C [auth E], D [auth F], G, H	DNA (5'-D(GPGPTPTPCPTPAPGPAPAPCPC)-3')	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, E [auth C], F [auth D]	IPR010542	Vertebrate heat shock transcription factor, C-terminal domain	Domain
A, B, E [auth C], F [auth D]	IPR000232	Heat shock factor (HSF)-type, DNA-binding	Domain
A, B, E [auth C], F [auth D]	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
A, B, E [auth C], F [auth D]	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
A, B, E [auth C], F [auth D]	IPR027725	Heat shock transcription factor family	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, E [auth C], F [auth D]	Pharos: Q00613	amyotrophic lateral sclerosis type 1 cancer AMYOTROPHIC LATERAL SCLEROSIS 1 benign neoplasm large cell medulloblastoma breast cancer plantar fibromatosis

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.