5EW5

Crystal Structure of Colicin E9 In Complex with Its Immunity Protein Im9

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	His-Me finger endonucleases	8042111	3001893	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	His-Me finger endonucleases	8042111	3001893	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	His-Me finger endonucleases	8042111	3001893	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	His-Me finger endonucleases	8042111	3001893	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Colicin E immunity proteins	8037581	3001814	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Colicin E immunity proteins	8037581	3001814	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Colicin E immunity proteins	8037581	3001814	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Colicin E immunity proteins	8037581	3001814	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF03515	e5ew5A1	A: beta complex topology	X: Cloacin translocation domain (From Topology)	H: Cloacin translocation domain (From Topology)	T: Cloacin translocation domain	F: PF03515	ECOD (1.6)
A	PF21431	e5ew5A2	A: few secondary structure elements	X: His-Me finger endonucleases (From Homology)	H: His-Me finger endonucleases	T: His-Me finger endonucleases	F: PF21431	ECOD (1.6)
B	PF03515	e5ew5B2	A: beta complex topology	X: Cloacin translocation domain (From Topology)	H: Cloacin translocation domain (From Topology)	T: Cloacin translocation domain	F: PF03515	ECOD (1.6)
B	PF21431	e5ew5B1	A: few secondary structure elements	X: His-Me finger endonucleases (From Homology)	H: His-Me finger endonucleases	T: His-Me finger endonucleases	F: PF21431	ECOD (1.6)
C	PF03515	e5ew5C1	A: beta complex topology	X: Cloacin translocation domain (From Topology)	H: Cloacin translocation domain (From Topology)	T: Cloacin translocation domain	F: PF03515	ECOD (1.6)
C	PF21431	e5ew5C2	A: few secondary structure elements	X: His-Me finger endonucleases (From Homology)	H: His-Me finger endonucleases	T: His-Me finger endonucleases	F: PF21431	ECOD (1.6)
D	PF03515	e5ew5D2	A: beta complex topology	X: Cloacin translocation domain (From Topology)	H: Cloacin translocation domain (From Topology)	T: Cloacin translocation domain	F: PF03515	ECOD (1.6)
D	PF21431	e5ew5D1	A: few secondary structure elements	X: His-Me finger endonucleases (From Homology)	H: His-Me finger endonucleases	T: His-Me finger endonucleases	F: PF21431	ECOD (1.6)
E	PF01320	e5ew5E1	A: alpha bundles	X: ACP-like	H: Colicin E immunity proteins (From Topology)	T: Colicin E immunity proteins	F: PF01320	ECOD (1.6)
F	PF01320	e5ew5F1	A: alpha bundles	X: ACP-like	H: Colicin E immunity proteins (From Topology)	T: Colicin E immunity proteins	F: PF01320	ECOD (1.6)
G	PF01320	e5ew5G1	A: alpha bundles	X: ACP-like	H: Colicin E immunity proteins (From Topology)	T: Colicin E immunity proteins	F: PF01320	ECOD (1.6)
H	PF01320	e5ew5H1	A: alpha bundles	X: ACP-like	H: Colicin E immunity proteins (From Topology)	T: Colicin E immunity proteins	F: PF01320	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
E	1.10.1200.20	Mainly Alpha	Orthogonal Bundle	Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein	Chain A	CATH (4.3.0)
F	1.10.1200.20	Mainly Alpha	Orthogonal Bundle	Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein	Chain A	CATH (4.3.0)
G	1.10.1200.20	Mainly Alpha	Orthogonal Bundle	Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein	Chain A	CATH (4.3.0)
H	1.10.1200.20	Mainly Alpha	Orthogonal Bundle	Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein	Chain A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF21431	Colicin/Pyocin-S2, DNase domain (Col-Pyo_DNase)	Colicin/Pyocin-S2, DNase domain	This entry represents the DNAse domain from colicin/pyocin bacteriocins, such as pyocin-S2 from Pseudomonas aeruginosa and colicin E7/E9 from E. coli. This domain has an alpha/beta fold containing a Zn2+ ion binding site [1-5].	Domain
A, B, C, D	PF03515	Colicin-like bacteriocin tRNase domain (Cloacin)	Colicin-like bacteriocin tRNase domain	-	Family
A, B, C, D	PF11570	Coiled-coil receptor-binding R-domain of colicin E2 (E2R135)	Coiled-coil receptor-binding R-domain of colicin E2	-	Coiled-coil
E, F, G, H	PF01320	Colicin immunity protein / pyocin immunity protein (Colicin_Pyocin)	Colicin immunity protein / pyocin immunity protein	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Colicin-E9	protein binding binding protein domain specific binding endonuclease activity nuclease activity hydrolase activity, acting on ester bonds hydrolase activity catalytic activity cation binding ion binding metal ion binding small molecule binding	biological process involved in interspecies interaction between organisms cellular process disruption of cell in another organism disruption of anatomical structure in another organism killing of cells of another organism cell killing response to stimulus response to other organism defense response response to external stimulus response to biotic stimulus defense response to bacterium defense response to other organism response to stress biological process involved in interspecies interaction between organisms cellular process disruption of cell in another organism disruption of anatomical structure in another organism killing of cells of another organism cell killing response to stimulus response to other organism defense response response to external stimulus response to biotic stimulus defense response to bacterium defense response to other organism response to stress response to bacterium response to external biotic stimulus	intracellular anatomical structure extrachromosomal circular DNA cellular anatomical entity extrachromosomal DNA protein-containing complex
E, F, G, H	Colicin-E9 immunity protein	protein binding binding protein domain specific binding toxic substance binding	cellular metabolic process metabolic process cellular process bacteriocin immunity toxin metabolic process secondary metabolic process	protein-containing complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR024575	Cloacin/colicin	Family
A, B, C, D	IPR016128	Pyosin/cloacin translocation domain	Domain
A, B, C, D	IPR044925	His-Me finger superfamily	Homologous Superfamily
A, B, C, D	IPR024566	Colicin, receptor domain	Domain
A, B, C, D	IPR036302	Pyosin/cloacin translocation domain superfamily	Homologous Superfamily
A, B, C, D	IPR037146	Colicin/pyocin, DNase domain superfamily	Homologous Superfamily
E, F, G, H	IPR000290	Colicin immunity protein/pyocin immunity protein	Family
E, F, G, H	IPR035900	Colicin E immunity protein superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.