Annotations: 5EAY

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Nucleic acid-binding proteins	8037540	3000135	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Nucleic acid-binding proteins	8037540	3000135	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Nucleic acid-binding proteins	8037540	3000135	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Nucleic acid-binding proteins	8037540	3000135	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Rep-A_N	e5eayA1	A: beta barrels	X: OB-fold	H: Nucleic acid-binding proteins (From Topology)	T: Nucleic acid-binding proteins	F: Rep-A_N	ECOD (1.6)
B	Rep-A_N	e5eayB1	A: beta barrels	X: OB-fold	H: Nucleic acid-binding proteins (From Topology)	T: Nucleic acid-binding proteins	F: Rep-A_N	ECOD (1.6)
C	Rep-A_N	e5eayC1	A: beta barrels	X: OB-fold	H: Nucleic acid-binding proteins (From Topology)	T: Nucleic acid-binding proteins	F: Rep-A_N	ECOD (1.6)
D	Rep-A_N	e5eayD1	A: beta barrels	X: OB-fold	H: Nucleic acid-binding proteins (From Topology)	T: Nucleic acid-binding proteins	F: Rep-A_N	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.40.50.140	Mainly Beta	Beta Barrel	OB fold (Dihydrolipoamide Acetyltransferase, E2P)	Nucleic acid-binding proteins	CATH (4.3.0)
B	2.40.50.140	Mainly Beta	Beta Barrel	OB fold (Dihydrolipoamide Acetyltransferase, E2P)	Nucleic acid-binding proteins	CATH (4.3.0)
C	2.40.50.140	Mainly Beta	Beta Barrel	OB fold (Dihydrolipoamide Acetyltransferase, E2P)	Nucleic acid-binding proteins	CATH (4.3.0)
D	2.40.50.140	Mainly Beta	Beta Barrel	OB fold (Dihydrolipoamide Acetyltransferase, E2P)	Nucleic acid-binding proteins	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF04057	Replication factor-A protein 1, N-terminal domain (Rep-A_N)	Replication factor-A protein 1, N-terminal domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Replication protein A 70 kDa DNA-binding subunit	chromatin-protein adaptor activity protein-macromolecule adaptor activity molecular adaptor activity DNA binding binding organic cyclic compound binding damaged DNA binding nucleic acid binding sequence-specific DNA binding G-rich strand telomeric DNA binding sequence-specific single stranded DNA binding single-stranded telomeric DNA binding single-stranded DNA binding telomeric DNA binding chromatin-protein adaptor activity protein-macromolecule adaptor activity molecular adaptor activity DNA binding binding organic cyclic compound binding damaged DNA binding nucleic acid binding sequence-specific DNA binding G-rich strand telomeric DNA binding sequence-specific single stranded DNA binding single-stranded telomeric DNA binding single-stranded DNA binding telomeric DNA binding cation binding ion binding metal ion binding small molecule binding	cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA replication organic substance metabolic process metabolic process primary metabolic process DNA metabolic process macromolecule metabolic process cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA replication organic substance metabolic process metabolic process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process protein localization localization cellular localization protein localization to chromosome protein localization to organelle cellular macromolecule localization macromolecule localization cellular biosynthetic process telomere organization RNA-templated DNA biosynthetic process telomere maintenance via telomere lengthening cellular component organization nucleic acid biosynthetic process organic substance biosynthetic process cellular component organization or biogenesis telomere maintenance via telomerase cellular metabolic process chromosome organization nucleobase-containing compound biosynthetic process macromolecule biosynthetic process telomere maintenance organelle organization biosynthetic process organic cyclic compound biosynthetic process DNA biosynthetic process mismatch repair DNA repair DNA-templated DNA replication protein localization to site of double-strand break DNA recombination double-strand break repair recombinational repair double-strand break repair via homologous recombination base-excision repair nucleotide-excision repair sexual reproduction reproductive process meiotic cell cycle cell cycle	membrane-bounded organelle protein-containing complex intracellular anatomical structure nucleus membrane-enclosed lumen nuclear protein-containing complex nuclear chromosome chromosome intracellular organelle lumen intracellular organelle nuclear replisome replication fork non-membrane-bounded organelle DNA replication factor A complex membrane-bounded organelle protein-containing complex intracellular anatomical structure nucleus membrane-enclosed lumen nuclear protein-containing complex nuclear chromosome chromosome intracellular organelle lumen intracellular organelle nuclear replisome replication fork non-membrane-bounded organelle DNA replication factor A complex protein-DNA complex nuclear replication fork intracellular membrane-bounded organelle cellular anatomical entity organelle lumen organelle intracellular non-membrane-bounded organelle nuclear lumen replisome nucleoplasm site of double-strand break site of DNA damage chromosome, telomeric region chromosomal region nuclear body PML body
E, F, G, H	DNA replication ATP-dependent helicase/nuclease DNA2	ATP-dependent activity, acting on DNA DNA helicase activity catalytic activity, acting on DNA 5'-3' DNA helicase activity catalytic activity, acting on a nucleic acid helicase activity catalytic activity ATP-dependent activity nuclease activity hydrolase activity, acting on ester bonds hydrolase activity 5'-flap endonuclease activity endonuclease activity endodeoxyribonuclease activity, producing 5'-phosphomonoesters ATP-dependent activity, acting on DNA DNA helicase activity catalytic activity, acting on DNA 5'-3' DNA helicase activity catalytic activity, acting on a nucleic acid helicase activity catalytic activity ATP-dependent activity nuclease activity hydrolase activity, acting on ester bonds hydrolase activity 5'-flap endonuclease activity endonuclease activity endodeoxyribonuclease activity, producing 5'-phosphomonoesters DNA nuclease activity DNA endonuclease activity flap endonuclease activity endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters RNA binding binding organic cyclic compound binding nucleic acid binding iron-sulfur cluster binding 4 iron, 4 sulfur cluster binding metal cluster binding small molecule binding site-specific endodeoxyribonuclease activity, specific for altered base adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding DNA binding pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity, acting on acid anhydrides single-stranded DNA helicase activity cation binding metal ion binding	nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA replication organic substance metabolic process metabolic process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process cellular response to stimulus double-strand break repair response to stimulus DNA double-strand break processing cellular response to stress nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA replication organic substance metabolic process metabolic process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process cellular response to stimulus double-strand break repair response to stimulus DNA double-strand break processing cellular response to stress DNA damage response response to stress cellular process DNA repair DNA replication, removal of RNA primer catabolic process RNA metabolic process macromolecule catabolic process organic cyclic compound catabolic process nucleobase-containing compound catabolic process nucleic acid catabolic process organic substance catabolic process RNA catabolic process DNA-templated DNA replication DNA-templated DNA replication maintenance of fidelity replication fork reversal replication fork processing positive regulation of DNA replication positive regulation of biological process regulation of primary metabolic process positive regulation of metabolic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process positive regulation of nucleobase-containing compound metabolic process regulation of biological process biological regulation regulation of macromolecule metabolic process positive regulation of DNA metabolic process regulation of metabolic process regulation of DNA metabolic process regulation of DNA replication cellular component organization G-quadruplex DNA unwinding organelle organization DNA geometric change chromosome organization DNA conformation change cellular component organization or biogenesis mitochondrial genome maintenance mitochondrial DNA metabolic process mitochondrial DNA replication mitochondrion organization formation of extrachromosomal circular DNA telomere organization telomere maintenance cellular component biogenesis cellular component assembly telomere maintenance via telomere trimming t-circle formation nuclear DNA replication cell cycle DNA replication telomere maintenance via semi-conservative replication cell cycle cell cycle process regulation of cellular process regulation of cell cycle phase transition signaling negative regulation of biological process negative regulation of cell cycle cell cycle phase transition intracellular signal transduction cell communication negative regulation of cellular process negative regulation of cell cycle phase transition signal transduction DNA replication checkpoint signaling regulation of cell cycle regulation of cell cycle process DNA integrity checkpoint signaling negative regulation of cell cycle process cell cycle checkpoint signaling lagging strand elongation DNA strand elongation DNA replication, Okazaki fragment processing DNA strand elongation involved in DNA replication base-excision repair mitochondrial DNA repair mitotic cell cycle mitotic telomere maintenance via semi-conservative replication mitotic cell cycle process	membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle nucleus membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle nucleus membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen nucleoid mitochondrial matrix intracellular non-membrane-bounded organelle mitochondrial nucleoid non-membrane-bounded organelle chromosome chromosome, telomeric region chromosomal region

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR007199	Replication factor-A protein 1, N-terminal	Domain
A, B, C, D	IPR047192	Replication factor A, C-terminal, eukaryotes	Domain
A, B, C, D	IPR013955	Replication factor A, C-terminal	Domain
A, B, C, D	IPR031657	Replication protein A, OB domain	Domain
A, B, C, D	IPR004365	OB-fold nucleic acid binding domain, AA-tRNA synthetase-type	Domain
A, B, C, D	IPR012340	Nucleic acid-binding, OB-fold	Homologous Superfamily
A, B, C, D	IPR004591	Replication factor A protein 1	Family
E, F, G, H	IPR041677	DNA2/NAM7 helicase, helicase domain	Domain
E, F, G, H	IPR026851	DNA replication ATP-dependent helicase/nuclease Dna2/JHS1, DEXXQ-box helicase domain	Domain
E, F, G, H	IPR041679	DNA2/NAM7 helicase-like, C-terminal	Domain
E, F, G, H	IPR048459	DNA2, rift barrel domain	Domain
E, F, G, H	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
E, F, G, H	IPR014808	DNA replication factor Dna2, N-terminal	Domain
E, F, G, H	IPR011604	PD-(D/E)XK endonuclease-like domain superfamily	Homologous Superfamily
E, F, G, H	IPR047187	Upf1-like, C-terminal helicase domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C, D	Pharos: P27694	Chloracne astrocytic tumor breast cancer glioblastoma malignant ependymoma ovarian cancer atypical teratoid rhabdoid tumor Hodgkins lymphoma malignant mesothelioma psoriasis B-cell chronic lymphocytic leukemia dermatomyositis lymphoplasmacytic lymphoma medulloblastoma
E, F, G, H	Pharos: P51530	mitochondrial DNA deletion syndrome with progressive myopathy progressive external ophthalmoplegia Seckel syndrome 8 intellectual disability breast cancer isolated growth hormone deficiency type IA malignant colon neoplasm microcephaly muscle tissue disease nasopharyngeal carcinoma Primary microcephaly psoriasis cerebrovascular disorder large cell medulloblastoma malignant mesothelioma mitochondrial DNA deletion syndrome with progressive myopathy progressive external ophthalmoplegia Seckel syndrome 8 intellectual disability breast cancer isolated growth hormone deficiency type IA malignant colon neoplasm microcephaly muscle tissue disease nasopharyngeal carcinoma Primary microcephaly psoriasis cerebrovascular disorder large cell medulloblastoma malignant mesothelioma muscular disease non-small cell lung carcinoma primitive neuroectodermal tumor lung cancer medulloblastoma myopathy ovarian cancer

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.