5E4H

Crystal Structure of Apoenzyme Alpha-kinase Domain of Myosin-II Heavy Chain Kinase A

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Protein Modification Annotation

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
G	Alpha_kinase	e5e4hG1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Alpha_kinase	ECOD (1.6)
A	Alpha_kinase	e5e4hA1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Alpha_kinase	ECOD (1.6)
B	Alpha_kinase	e5e4hB1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Alpha_kinase	ECOD (1.6)
C	Alpha_kinase	e5e4hC1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Alpha_kinase	ECOD (1.6)
D	Alpha_kinase	e5e4hD1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Alpha_kinase	ECOD (1.6)
E	Alpha_kinase	e5e4hE1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Alpha_kinase	ECOD (1.6)
F	Alpha_kinase	e5e4hF1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Alpha_kinase	ECOD (1.6)
H	Alpha_kinase	e5e4hH1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Alpha_kinase	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
G	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
G	3.20.200.10	Alpha Beta	Alpha-Beta Barrel	Protein kinase-like fold	MHCK/EF2 kinase	CATH (4.3.0)
A	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
A	3.20.200.10	Alpha Beta	Alpha-Beta Barrel	Protein kinase-like fold	MHCK/EF2 kinase	CATH (4.3.0)
B	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
B	3.20.200.10	Alpha Beta	Alpha-Beta Barrel	Protein kinase-like fold	MHCK/EF2 kinase	CATH (4.3.0)
C	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
C	3.20.200.10	Alpha Beta	Alpha-Beta Barrel	Protein kinase-like fold	MHCK/EF2 kinase	CATH (4.3.0)
D	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
D	3.20.200.10	Alpha Beta	Alpha-Beta Barrel	Protein kinase-like fold	MHCK/EF2 kinase	CATH (4.3.0)
E	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
E	3.20.200.10	Alpha Beta	Alpha-Beta Barrel	Protein kinase-like fold	MHCK/EF2 kinase	CATH (4.3.0)
F	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
F	3.20.200.10	Alpha Beta	Alpha-Beta Barrel	Protein kinase-like fold	MHCK/EF2 kinase	CATH (4.3.0)
H	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
H	3.20.200.10	Alpha Beta	Alpha-Beta Barrel	Protein kinase-like fold	MHCK/EF2 kinase	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G, H	PF02816	Alpha-kinase family (Alpha_kinase)	Alpha-kinase family	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G, H	Myosin-II heavy chain kinase A	cytoskeletal protein binding actin binding protein-containing complex binding protein binding binding actin filament binding protein serine/threonine kinase activity catalytic activity, acting on a protein myosin heavy chain kinase activity calmodulin-dependent protein kinase activity kinase activity protein kinase activity transferase activity catalytic activity cytoskeletal protein binding actin binding protein-containing complex binding protein binding binding actin filament binding protein serine/threonine kinase activity catalytic activity, acting on a protein myosin heavy chain kinase activity calmodulin-dependent protein kinase activity kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups myosin II binding myosin binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding cation binding AMP binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding purine ribonucleoside triphosphate binding ATP binding ADP binding pyrophosphatase activity nucleoside diphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity ADP phosphatase activity hydrolase activity, acting on acid anhydrides cyclic nucleotide binding cAMP binding identical protein binding	cellular component disassembly actin cytoskeleton organization protein-containing complex organization myosin filament organization protein-containing complex disassembly myosin filament disassembly cellular component organization myosin II filament organization myosin II filament disassembly organelle organization actin filament-based process cellular process cellular component organization or biogenesis supramolecular fiber organization cellular component disassembly actin cytoskeleton organization protein-containing complex organization myosin filament organization protein-containing complex disassembly myosin filament disassembly cellular component organization myosin II filament organization myosin II filament disassembly organelle organization actin filament-based process cellular process cellular component organization or biogenesis supramolecular fiber organization cytoskeleton organization mitotic cell cycle process cytoskeleton-dependent cytokinesis mitotic cytokinesis cytokinesis cell division cell cycle mitotic cell cycle cell cycle process protein phosphorylation organonitrogen compound metabolic process protein autophosphorylation phosphorylation cellular metabolic process primary metabolic process organic substance metabolic process macromolecule modification phosphate-containing compound metabolic process metabolic process protein metabolic process macromolecule metabolic process protein modification process phosphorus metabolic process chemotaxis response to stimulus taxis response to chemical response to external stimulus locomotion actin filament organization actin crosslink formation cellular component biogenesis cellular component assembly actin filament bundle organization actin filament bundle assembly	cell periphery cell cortex intracellular anatomical structure cytoplasm cellular anatomical entity contractile ring cell division site cortical cytoskeleton actomyosin contractile ring actin cytoskeleton intracellular organelle cytoskeleton organelle intracellular non-membrane-bounded organelle cell periphery cell cortex intracellular anatomical structure cytoplasm cellular anatomical entity contractile ring cell division site cortical cytoskeleton actomyosin contractile ring actin cytoskeleton intracellular organelle cytoskeleton organelle intracellular non-membrane-bounded organelle cortical actin cytoskeleton non-membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G, H	IPR019775	WD40 repeat, conserved site	Conserved Site
A, B, C, D, E A, B, C, D, E, F, G, H	IPR004166	Alpha-type protein kinase, alpha-kinase domain	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	IPR015943	WD40/YVTN repeat-like-containing domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR036322	WD40-repeat-containing domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR001680	WD40 repeat	Repeat
A, B, C, D, E A, B, C, D, E, F, G, H	IPR020472	G-protein beta WD-40 repeat	Repeat

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
A, B, C, D, E A, B, C, D, E, F, G, H	PHD	Parent Component: ASP RESID: AA0033 PSI-MOD : L-aspartic 4-phosphoric anhydride MOD:00042
A, B, C, D, E A, B, C, D, E, F, G, H	TPO	Parent Component: THR RESID: AA0033 , AA0038 PSI-MOD : L-aspartic 4-phosphoric anhydride MOD:00042 , O-phospho-L-threonine MOD:00047

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.