5BKF

Cyro-EM structure of human Glycine Receptor alpha2-beta heteromer, Glycine bound, desensitized state

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF02931	e5bkfA1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Nicotinic receptor ligand binding domain-like (From Topology)	T: Nicotinic receptor ligand binding domain-like	F: PF02931	ECOD (1.6)
A	PF02932	e5bkfA2	A: alpha bundles	X: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	H: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	T: Neurotransmitter-gated ion-channel transmembrane pore	F: PF02932	ECOD (1.6)
B	PF02931	e5bkfB1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Nicotinic receptor ligand binding domain-like (From Topology)	T: Nicotinic receptor ligand binding domain-like	F: PF02931	ECOD (1.6)
B	PF02932	e5bkfB2	A: alpha bundles	X: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	H: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	T: Neurotransmitter-gated ion-channel transmembrane pore	F: PF02932	ECOD (1.6)
C	PF02931	e5bkfC1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Nicotinic receptor ligand binding domain-like (From Topology)	T: Nicotinic receptor ligand binding domain-like	F: PF02931	ECOD (1.6)
C	PF02932	e5bkfC2	A: alpha bundles	X: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	H: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	T: Neurotransmitter-gated ion-channel transmembrane pore	F: PF02932	ECOD (1.6)
D	PF02931	e5bkfD1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Nicotinic receptor ligand binding domain-like (From Topology)	T: Nicotinic receptor ligand binding domain-like	F: PF02931	ECOD (1.6)
D	PF02932	e5bkfD2	A: alpha bundles	X: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	H: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	T: Neurotransmitter-gated ion-channel transmembrane pore	F: PF02932	ECOD (1.6)
E	PF02931	e5bkfE1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Nicotinic receptor ligand binding domain-like (From Topology)	T: Nicotinic receptor ligand binding domain-like	F: PF02931	ECOD (1.6)
E	PF02932	e5bkfE2	A: alpha bundles	X: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	H: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	T: Neurotransmitter-gated ion-channel transmembrane pore	F: PF02932	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF02931	Neurotransmitter-gated ion-channel ligand binding domain (Neur_chan_LBD)	Neurotransmitter-gated ion-channel ligand binding domain	-	Family
E	PF02932	Neurotransmitter-gated ion-channel transmembrane region (Neur_chan_memb)	Neurotransmitter-gated ion-channel transmembrane region	-	Family
E	PF02931	Neurotransmitter-gated ion-channel ligand binding domain (Neur_chan_LBD)	Neurotransmitter-gated ion-channel ligand binding domain	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Glycine receptor subunit alpha-2	transmitter-gated monoatomic ion channel activity monoatomic anion transmembrane transporter activity chloride transmembrane transporter activity molecular transducer activity monoatomic ion channel activity neurotransmitter receptor activity ligand-gated channel activity passive transmembrane transporter activity inorganic molecular entity transmembrane transporter activity transporter activity gated channel activity signaling receptor activity glycine-gated chloride ion channel activity ligand-gated monoatomic anion channel activity transmitter-gated monoatomic ion channel activity monoatomic anion transmembrane transporter activity chloride transmembrane transporter activity molecular transducer activity monoatomic ion channel activity neurotransmitter receptor activity ligand-gated channel activity passive transmembrane transporter activity inorganic molecular entity transmembrane transporter activity transporter activity gated channel activity signaling receptor activity glycine-gated chloride ion channel activity ligand-gated monoatomic anion channel activity ligand-gated monoatomic ion channel activity extracellular ligand-gated monoatomic ion channel activity channel activity transmitter-gated channel activity monoatomic ion transmembrane transporter activity monoatomic anion channel activity transmembrane transporter activity inorganic anion transmembrane transporter activity chloride channel activity inhibitory extracellular ligand-gated monoatomic ion channel activity excitatory extracellular ligand-gated monoatomic ion channel activity extracellularly glycine-gated ion channel activity extracellularly glycine-gated chloride channel activity cation binding organic acid binding binding ion binding anion binding glycine binding amino acid binding carboxylic acid binding small molecule binding transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential postsynaptic neurotransmitter receptor activity neurotransmitter receptor activity involved in regulation of postsynaptic membrane potential metal ion binding transmembrane signaling receptor activity	response to acid chemical response to organic substance response to stimulus response to chemical response to amino acid cellular response to stimulus regulation of biological process regulation of cellular process signaling biological regulation cell communication G protein-coupled receptor signaling pathway cellular process signal transduction response to acid chemical response to organic substance response to stimulus response to chemical response to amino acid cellular response to stimulus regulation of biological process regulation of cellular process signaling biological regulation cell communication G protein-coupled receptor signaling pathway cellular process signal transduction neuropeptide signaling pathway transport monoatomic ion transmembrane transport inorganic ion transmembrane transport inorganic anion transmembrane transport establishment of localization chloride transport transmembrane transport localization chloride transmembrane transport monoatomic anion transport monoatomic anion transmembrane transport monoatomic ion transport inorganic anion transport cellular response to acid chemical cellular response to amino acid stimulus cellular response to chemical stimulus response to alcohol response to oxygen-containing compound cellular response to oxygen-containing compound cellular response to alcohol cellular response to organic substance cellular response to ethanol response to ethanol cellular response to inorganic substance cellular response to metal ion response to zinc ion response to inorganic substance response to metal ion cellular response to zinc ion	protein-containing complex transmembrane transporter complex membrane chloride channel complex membrane protein complex cellular anatomical entity transporter complex monoatomic ion channel complex cell periphery postsynapse postsynaptic membrane synapse cell junction plasma membrane protein-containing complex transmembrane transporter complex membrane chloride channel complex membrane protein complex cellular anatomical entity transporter complex monoatomic ion channel complex cell periphery postsynapse postsynaptic membrane synapse cell junction plasma membrane plasma membrane region synaptic membrane glycinergic synapse membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle intracellular membrane-bounded organelle cell projection neuron projection plasma membrane bounded cell projection
E	Glycine receptor subunit beta,Green fluorescent protein	monoatomic anion transmembrane transporter activity chloride transmembrane transporter activity gated channel activity monoatomic ion channel activity inhibitory extracellular ligand-gated monoatomic ion channel activity excitatory extracellular ligand-gated monoatomic ion channel activity ligand-gated monoatomic anion channel activity ligand-gated channel activity ligand-gated monoatomic ion channel activity extracellular ligand-gated monoatomic ion channel activity extracellularly glycine-gated ion channel activity passive transmembrane transporter activity inorganic molecular entity transmembrane transporter activity channel activity monoatomic ion transmembrane transporter activity monoatomic anion transmembrane transporter activity chloride transmembrane transporter activity gated channel activity monoatomic ion channel activity inhibitory extracellular ligand-gated monoatomic ion channel activity excitatory extracellular ligand-gated monoatomic ion channel activity ligand-gated monoatomic anion channel activity ligand-gated channel activity ligand-gated monoatomic ion channel activity extracellular ligand-gated monoatomic ion channel activity extracellularly glycine-gated ion channel activity passive transmembrane transporter activity inorganic molecular entity transmembrane transporter activity channel activity monoatomic ion transmembrane transporter activity monoatomic anion channel activity transporter activity extracellularly glycine-gated chloride channel activity transmembrane transporter activity inorganic anion transmembrane transporter activity chloride channel activity cation binding organic acid binding binding ion binding anion binding glycine binding amino acid binding carboxylic acid binding small molecule binding protein-containing complex binding transmitter-gated monoatomic ion channel activity molecular transducer activity transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential signaling receptor activity neurotransmitter receptor activity postsynaptic neurotransmitter receptor activity neurotransmitter receptor activity involved in regulation of postsynaptic membrane potential transmitter-gated channel activity transmembrane signaling receptor activity	bioluminescence cellular metabolic process metabolic process cellular process generation of precursor metabolites and energy cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation cell communication G protein-coupled receptor signaling pathway signal transduction bioluminescence cellular metabolic process metabolic process cellular process generation of precursor metabolites and energy cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation cell communication G protein-coupled receptor signaling pathway signal transduction neuropeptide signaling pathway membrane organization protein localization cellular localization gamma-aminobutyric acid receptor clustering postsynapse organization neurotransmitter-gated ion channel clustering localization within membrane protein localization to membrane cellular component organization localization cell junction organization receptor clustering cellular macromolecule localization macromolecule localization cellular component organization or biogenesis synapse organization postsynaptic membrane organization trans-synaptic signaling cell-cell signaling synaptic transmission, glycinergic chemical synaptic transmission synaptic signaling anterograde trans-synaptic signaling transport monoatomic ion transmembrane transport inorganic ion transmembrane transport inorganic anion transmembrane transport establishment of localization chloride transport transmembrane transport chloride transmembrane transport monoatomic anion transport monoatomic anion transmembrane transport monoatomic ion transport inorganic anion transport reflex response to external stimulus righting reflex startle response multicellular organismal process system process nervous system process neuromuscular process sexual reproduction acrosome reaction reproductive process fertilization single fertilization developmental process multicellular organism development anatomical structure development nervous system development system development sensory perception of light stimulus sensory perception visual perception adult walking behavior adult locomotory behavior behavior adult behavior locomotory behavior walking behavior	cell periphery postsynapse postsynaptic membrane synapse cell junction membrane plasma membrane plasma membrane region cellular anatomical entity synaptic membrane glycinergic synapse protein-containing complex plasma membrane protein complex membrane protein complex cell periphery postsynapse postsynaptic membrane synapse cell junction membrane plasma membrane plasma membrane region cellular anatomical entity synaptic membrane glycinergic synapse protein-containing complex plasma membrane protein complex membrane protein complex transporter complex transmembrane transporter complex chloride channel complex glycine-gated chloride channel complex monoatomic ion channel complex somatodendritic compartment cell projection dendrite dendritic tree neuron projection plasma membrane bounded cell projection intracellular anatomical structure cytoplasm postsynaptic specialization organelle GABA-ergic synapse

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR008129	Glycine receptor alpha2	Family
A, B, C, D	IPR006202	Neurotransmitter-gated ion-channel ligand-binding domain	Domain
A, B, C, D	IPR036719	Neurotransmitter-gated ion-channel transmembrane domain superfamily	Homologous Superfamily
A, B, C, D	IPR006028	Gamma-aminobutyric acid A receptor/Glycine receptor alpha	Family
A, B, C, D	IPR008127	Glycine receptor alpha	Family
A, B, C, D	IPR006029	Neurotransmitter-gated ion-channel transmembrane domain	Domain
A, B, C, D	IPR036734	Neurotransmitter-gated ion-channel ligand-binding domain superfamily	Homologous Superfamily
A, B, C, D	IPR018000	Neurotransmitter-gated ion-channel, conserved site	Conserved Site
A, B, C, D	IPR038050	Neuronal acetylcholine receptor	Homologous Superfamily
A, B, C, D	IPR006201	Neurotransmitter-gated ion-channel	Family
E	IPR000786	Green fluorescent protein, GFP	Family
E	IPR011584	Green fluorescent protein-related	Family
E	IPR009017	Green fluorescent protein	Homologous Superfamily
E	IPR006202	Neurotransmitter-gated ion-channel ligand-binding domain	Domain
E	IPR036719	Neurotransmitter-gated ion-channel transmembrane domain superfamily	Homologous Superfamily
E	IPR047031	Glycine receptor subunit beta, extracellular domain	Domain
E	IPR006029	Neurotransmitter-gated ion-channel transmembrane domain	Domain
E	IPR036734	Neurotransmitter-gated ion-channel ligand-binding domain superfamily	Homologous Superfamily
E	IPR018000	Neurotransmitter-gated ion-channel, conserved site	Conserved Site
E	IPR038050	Neuronal acetylcholine receptor	Homologous Superfamily
E	IPR008060	Glycine receptor beta	Family
E	IPR047029	Glycine receptor subunit beta, transmembrane domain	Domain
E	IPR006201	Neurotransmitter-gated ion-channel	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C, D	Pharos: P23416	hereditary hyperekplexia medulloblastoma schizophrenia intellectual disability onchocerciasis strongyloidiasis large cell medulloblastoma psoriasis
E	Pharos: P48167	hyperekplexia 2 hereditary hyperekplexia medulloblastoma ovarian cancer Pick disease subependymal giant cell astrocytoma adenoma atypical teratoid rhabdoid tumor Epileptic encephalopathy hyperekplexia 1 large cell medulloblastoma malignant ependymoma oligodendroglioma pancreatic intraductal papillary-mucinous carcinoma primitive neuroectodermal tumor hyperekplexia 2 hereditary hyperekplexia medulloblastoma ovarian cancer Pick disease subependymal giant cell astrocytoma adenoma atypical teratoid rhabdoid tumor Epileptic encephalopathy hyperekplexia 1 large cell medulloblastoma malignant ependymoma oligodendroglioma pancreatic intraductal papillary-mucinous carcinoma primitive neuroectodermal tumor astrocytic tumor breast cancer lung cancer malignant glioma osteosarcoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia psoriasis atopic eczema ductal breast carcinoma in situ glioblastoma hydrolethalus syndrome lung carcinoma