Annotations: 5AUN

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	HypA-like	8048619	4006166	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	HypA-like	8057059	3002385	SCOP2 (2022-06-29)
B	SCOP2 Family	FlhG-like	8045215	4003984	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Simibi-like P-loop NTPases	8057058	3002018	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	HypA	e5aunA2	A: a+b three layers	X: Bacillus chorismate mutase-like	H: HypA Ni-binding domain (From Topology)	T: HypA Ni-binding domain	F: HypA	ECOD (1.6)
A	HypA_1	e5aunA1	A: few secondary structure elements	X: Rubredoxin-like	H: Rubredoxin-related	T: Rubredoxin-related	F: HypA_1	ECOD (1.6)
B	AAA_31	e5aunB1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: AAA_31	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.2320.50	Alpha Beta	2-Layer Sandwich	hypothetical protein PF0899 fold		CATH (4.3.0)
B	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF01155	Hydrogenase/urease nickel incorporation, metallochaperone, hypA (HypA)	Hydrogenase/urease nickel incorporation, metallochaperone, hypA	HypA is a metallochaperone that binds nickel to bring it safely to its target. The targets for Hypa are the nickel-containing enzymes [Ni,Fe]-hydrogenase and urease. The nickel coordinates with four nitrogens within the protein. The four conserved cy ...	HypA is a metallochaperone that binds nickel to bring it safely to its target. The targets for Hypa are the nickel-containing enzymes [Ni,Fe]-hydrogenase and urease. The nickel coordinates with four nitrogens within the protein. The four conserved cysteines towards the C-terminus bind one zinc moiety probably to stabilise the protein fold [1].	Domain
B	PF10609	NUBPL iron-transfer P-loop NTPase (ParA)	NUBPL iron-transfer P-loop NTPase	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Probable hydrogenase nickel incorporation protein HypA	cation binding transition metal ion binding ion binding binding nickel cation binding metal ion binding small molecule binding zinc ion binding	organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process biosynthetic process organic substance biosynthetic process metabolic process cellular process protein metabolic process macromolecule metabolic process organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process biosynthetic process organic substance biosynthetic process metabolic process cellular process protein metabolic process macromolecule metabolic process macromolecule modification protein modification process	-
B	ATPase involved in chromosome partitioning, ParA/MinD family, Mrp homolog	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity ATP-dependent activity ATP-dependent FeS chaperone activity metallochaperone activity molecular carrier activity iron-sulfur cluster binding 4 iron, 4 sulfur cluster binding metal cluster binding cation binding metal ion binding	cellular component biogenesis cellular component organization metallo-sulfur cluster assembly cellular component assembly iron-sulfur cluster assembly cellular process cellular component organization or biogenesis	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR020538	Hydrogenase nickel incorporation protein HypA/HybF, conserved site	Conserved Site
A	IPR000688	Hydrogenase maturation factor HypA/HybF	Family
B	IPR033756	Flagellum site-determining protein YlxH/ Fe-S cluster assembling factor NBP35	Family
B	IPR019591	Mrp/NBP35 ATP-binding protein	Family
B	IPR044304	Iron-sulfur protein NUBPL-like	Family
B	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.