5AKM

THE CRYSTAL STRUCTURE OF I-DMOI G20S IN COMPLEX WITH ITS TARGET DNA IN THE PRESENCE OF 2MM MG

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Homing endonucleases	8035762	3000266	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Homing endonucleases	8035767	3000266	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Homing endonucleases	8035767	3000266	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Homing endonucleases	8035762	3000266	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Homing endonucleases	8035767	3000266	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Homing endonucleases	8035762	3000266	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	LAGLIDADG_3	e5akmA2	A: a+b two layers	X: Homing endonucleases-like	H: Homing endonucleases (From Topology)	T: Homing endonucleases	F: LAGLIDADG_3	ECOD (1.6)
F	LAGLIDADG_3	e5akmF2	A: a+b two layers	X: Homing endonucleases-like	H: Homing endonucleases (From Topology)	T: Homing endonucleases	F: LAGLIDADG_3	ECOD (1.6)
K	LAGLIDADG_3	e5akmK2	A: a+b two layers	X: Homing endonucleases-like	H: Homing endonucleases (From Topology)	T: Homing endonucleases	F: LAGLIDADG_3	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.10.28.10	Alpha Beta	Roll	Endonuclease I-creI	Homing endonucleases	CATH (4.3.0)
F	3.10.28.10	Alpha Beta	Roll	Endonuclease I-creI	Homing endonucleases	CATH (4.3.0)
K	3.10.28.10	Alpha Beta	Roll	Endonuclease I-creI	Homing endonucleases	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, F, K	PF14528	LAGLIDADG-like domain (LAGLIDADG_3)	LAGLIDADG-like domain	This domain is part of the LAGLIDADG superfamily [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, F, K	HOMING ENDONUCLEASE I-DMOI	endonuclease activity nuclease activity hydrolase activity, acting on ester bonds hydrolase activity catalytic activity	intein-mediated protein splicing organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process biosynthetic process proteolysis organic substance biosynthetic process protein processing metabolic process intein-mediated protein splicing organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process biosynthetic process proteolysis organic substance biosynthetic process protein processing metabolic process cellular process protein metabolic process macromolecule metabolic process protein splicing nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA recombination DNA metabolic process nucleic acid metabolic process intron homing	-
B, G, L	5'-D(GPCPCPTPTPGPCPCPGPGPGPTPAPAP)-3'	-	-	-
C, H, M	5'-D(GPTPTPCPCPGPGPCPGPCP*GP)-3'	-	-	-
D, I, N	5'-D(CPGPCPGPCPCPGPGPAPAPCPTPTPAP*CP)-3'	-	-	-
E, J, O	5'-D(CPCPGPGPCPAPAPGPGPCP)-3'	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, F, K	IPR004860	Homing endonuclease, LAGLIDADG	Domain
A, F, K	IPR006142	Intein	Domain
A, F, K	IPR004042	Intein DOD homing endonuclease	Domain
A, F, K	IPR027434	Homing endonuclease	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.