4ZJS

Crystal structure of a chimeric acetylcholine binding protein from Aplysia Californica (Ac-AChBP) containing the main immunogenic region (MIR) from the human alpha 1 subunit of the muscle nicotinic acetylcholine receptor in complex with anatoxin-A.

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d4zjsa_	All beta proteins	Nicotinic receptor ligand binding domain-like	Nicotinic receptor ligand binding domain-like	automated matches	automated matches	Human (Homo sapiens ) [TaxId: 9606 ], California sea hare (Aplysia californica ) [TaxId: 6500 ], Human (Homo sapiens ) [TaxId: 9606 ], California sea hare (Aplysia californica ) [TaxId: 6500 ],	SCOPe (2.08)
B	d4zjsb_	All beta proteins	Nicotinic receptor ligand binding domain-like	Nicotinic receptor ligand binding domain-like	automated matches	automated matches	Human (Homo sapiens ) [TaxId: 9606 ], California sea hare (Aplysia californica ) [TaxId: 6500 ], Human (Homo sapiens ) [TaxId: 9606 ], California sea hare (Aplysia californica ) [TaxId: 6500 ],	SCOPe (2.08)
C	d4zjsc_	All beta proteins	Nicotinic receptor ligand binding domain-like	Nicotinic receptor ligand binding domain-like	automated matches	automated matches	Human (Homo sapiens ) [TaxId: 9606 ], California sea hare (Aplysia californica ) [TaxId: 6500 ], Human (Homo sapiens ) [TaxId: 9606 ], California sea hare (Aplysia californica ) [TaxId: 6500 ],	SCOPe (2.08)
D	d4zjsd_	All beta proteins	Nicotinic receptor ligand binding domain-like	Nicotinic receptor ligand binding domain-like	automated matches	automated matches	Human (Homo sapiens ) [TaxId: 9606 ], California sea hare (Aplysia californica ) [TaxId: 6500 ], Human (Homo sapiens ) [TaxId: 9606 ], California sea hare (Aplysia californica ) [TaxId: 6500 ],	SCOPe (2.08)
E	d4zjse_	All beta proteins	Nicotinic receptor ligand binding domain-like	Nicotinic receptor ligand binding domain-like	automated matches	automated matches	Human (Homo sapiens ) [TaxId: 9606 ], California sea hare (Aplysia californica ) [TaxId: 6500 ], Human (Homo sapiens ) [TaxId: 9606 ], California sea hare (Aplysia californica ) [TaxId: 6500 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF02931	e4zjsA1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Nicotinic receptor ligand binding domain-like (From Topology)	T: Nicotinic receptor ligand binding domain-like	F: PF02931	ECOD (1.6)
B	PF02931	e4zjsB1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Nicotinic receptor ligand binding domain-like (From Topology)	T: Nicotinic receptor ligand binding domain-like	F: PF02931	ECOD (1.6)
C	PF02931	e4zjsC1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Nicotinic receptor ligand binding domain-like (From Topology)	T: Nicotinic receptor ligand binding domain-like	F: PF02931	ECOD (1.6)
D	PF02931	e4zjsD1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Nicotinic receptor ligand binding domain-like (From Topology)	T: Nicotinic receptor ligand binding domain-like	F: PF02931	ECOD (1.6)
E	PF02931	e4zjsE1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Nicotinic receptor ligand binding domain-like (From Topology)	T: Nicotinic receptor ligand binding domain-like	F: PF02931	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.70.170.10	Mainly Beta	Distorted Sandwich	Acetylcholine Binding Protein	Chain: A,	CATH (4.3.0)
B	2.70.170.10	Mainly Beta	Distorted Sandwich	Acetylcholine Binding Protein	Chain: A,	CATH (4.3.0)
C	2.70.170.10	Mainly Beta	Distorted Sandwich	Acetylcholine Binding Protein	Chain: A,	CATH (4.3.0)
D	2.70.170.10	Mainly Beta	Distorted Sandwich	Acetylcholine Binding Protein	Chain: A,	CATH (4.3.0)
E	2.70.170.10	Mainly Beta	Distorted Sandwich	Acetylcholine Binding Protein	Chain: A,	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E	PF02931	Neurotransmitter-gated ion-channel ligand binding domain (Neur_chan_LBD)	Neurotransmitter-gated ion-channel ligand binding domain	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E	Acetylcholine receptor subunit alpha,Soluble acetylcholine receptor,Acetylcholine receptor subunit alpha,Soluble acetylcholine receptor	neurotransmitter receptor activity postsynaptic neurotransmitter receptor activity molecular transducer activity signaling receptor activity transmembrane signaling receptor activity acetylcholine receptor activity cation binding acetylcholine binding ion binding binding small molecule binding passive transmembrane transporter activity monoatomic ion channel activity channel activity neurotransmitter receptor activity postsynaptic neurotransmitter receptor activity molecular transducer activity signaling receptor activity transmembrane signaling receptor activity acetylcholine receptor activity cation binding acetylcholine binding ion binding binding small molecule binding passive transmembrane transporter activity monoatomic ion channel activity channel activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity transmitter-gated monoatomic ion channel activity gated channel activity transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential ligand-gated channel activity ligand-gated monoatomic ion channel activity extracellular ligand-gated monoatomic ion channel activity neurotransmitter receptor activity involved in regulation of postsynaptic membrane potential transmitter-gated channel activity monoatomic cation transmembrane transporter activity acetylcholine-gated monoatomic cation-selective channel activity ligand-gated monoatomic cation channel activity excitatory extracellular ligand-gated monoatomic ion channel activity monoatomic cation channel activity metal ion binding identical protein binding protein binding	cellular component organization cell junction organization neuromuscular junction development cellular process synapse organization cellular component organization or biogenesis multicellular organismal movement musculoskeletal movement multicellular organismal process system process nervous system process neuromuscular process skeletal muscle contraction muscle contraction cellular component organization cell junction organization neuromuscular junction development cellular process synapse organization cellular component organization or biogenesis multicellular organismal movement musculoskeletal movement multicellular organismal process system process nervous system process neuromuscular process skeletal muscle contraction muscle contraction muscle system process striated muscle contraction developmental process developmental growth skeletal muscle tissue development animal organ development growth tissue development muscle structure development skeletal muscle organ development skeletal muscle tissue growth anatomical structure development muscle tissue development muscle organ development cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation signal transduction cell communication trans-synaptic signaling cell-cell signaling chemical synaptic transmission synaptic signaling anterograde trans-synaptic signaling neuromuscular synaptic transmission action potential transmission of nerve impulse neuronal action potential regulation of biological quality regulation of membrane potential response to chemical response to nicotine synaptic transmission, cholinergic cellular homeostasis homeostatic process muscle cell cellular homeostasis neuron cellular homeostasis	cell periphery postsynapse postsynaptic membrane synapse cell junction membrane plasma membrane plasma membrane region cellular anatomical entity synaptic membrane cell surface postsynaptic specialization membrane postsynaptic specialization organelle cell periphery postsynapse postsynaptic membrane synapse cell junction membrane plasma membrane plasma membrane region cellular anatomical entity synaptic membrane cell surface postsynaptic specialization membrane postsynaptic specialization organelle cell projection neuron projection plasma membrane bounded cell projection neuromuscular junction protein-containing complex plasma membrane protein complex membrane protein complex transporter complex receptor complex acetylcholine-gated channel complex transmembrane transporter complex plasma membrane signaling receptor complex monoatomic ion channel complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E	IPR006202	Neurotransmitter-gated ion-channel ligand-binding domain	Domain
A, B, C, D, E	IPR036719	Neurotransmitter-gated ion-channel transmembrane domain superfamily	Homologous Superfamily
A, B, C, D, E	IPR002394	Nicotinic acetylcholine receptor	Family
A, B, C, D, E	IPR006029	Neurotransmitter-gated ion-channel transmembrane domain	Domain
A, B, C, D, E	IPR036734	Neurotransmitter-gated ion-channel ligand-binding domain superfamily	Homologous Superfamily
A, B, C, D, E	IPR018000	Neurotransmitter-gated ion-channel, conserved site	Conserved Site
A, B, C, D, E	IPR038050	Neuronal acetylcholine receptor	Homologous Superfamily
A, B, C, D, E	IPR006201	Neurotransmitter-gated ion-channel	Family
A, B, C, D, E	IPR006202	Neurotransmitter-gated ion-channel ligand-binding domain	Domain
A, B, C, D, E	IPR036734	Neurotransmitter-gated ion-channel ligand-binding domain superfamily	Homologous Superfamily
A, B, C, D, E	IPR006201	Neurotransmitter-gated ion-channel	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C, D, E	Pharos: P02708	congenital myasthenic syndrome myasthenic syndrome, congenital, 1B, fast-channel lethal multiple pterygium syndrome multiple pterygium syndrome congenital myasthenic syndrome 1A Fibrosis myopathy peripheral neuropathy amyotrophic lateral sclerosis Becker muscular dystrophy Emery-Dreifuss muscular dystrophy malignant glioma non-immune hydrops fetalis pituitary cancer arthrogryposis congenital myasthenic syndrome myasthenic syndrome, congenital, 1B, fast-channel lethal multiple pterygium syndrome multiple pterygium syndrome congenital myasthenic syndrome 1A Fibrosis myopathy peripheral neuropathy amyotrophic lateral sclerosis Becker muscular dystrophy Emery-Dreifuss muscular dystrophy malignant glioma non-immune hydrops fetalis pituitary cancer arthrogryposis juvenile dermatomyositis limb-girdle muscular dystrophy medulloblastoma peripheral nervous system disease psoriasis ulcerative colitis atrial fibrillation Duchenne muscular dystrophy large cell medulloblastoma lung cancer

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.