4YN3

Crystal structure of Cucumisin complex with pro-peptide

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF17766	e4yn3A3	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF17766	ECOD (1.6)
A	F_UNCLASSIFIED	e4yn3A2	A: a/b three-layered sandwiches	X: The swivelling beta/beta/alpha domains	H: The swivelling beta/beta/alpha domain (From Topology)	T: The swivelling beta/beta/alpha domain	F: F_UNCLASSIFIED	ECOD (1.6)
A	PF00082	e4yn3A1	A: a/b three-layered sandwiches	X: Subtilisin-like	H: Subtilisin-like (From Topology)	T: Subtilisin-like	F: PF00082	ECOD (1.6)
B	PF05922	e4yn3B1	A: a+b two layers	X: Alpha-beta plaits	H: Protease propeptides/inhibitors (From Topology)	T: Protease propeptides/inhibitors	F: PF05922	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.200	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Peptidase S8/S53 domain	CATH (4.3.0)
A	3.50.30.30	Alpha Beta	3-Layer(bba) Sandwich	Glucose Oxidase	domain 1	CATH (4.3.0)
A	2.60.40.2310	Mainly Beta	Sandwich	Immunoglobulin-like		CATH (4.3.0)
B	3.30.70.80	Alpha Beta	2-Layer Sandwich	Alpha-Beta Plaits	Peptidase S8 propeptide/proteinase inhibitor I9	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00082	Subtilase family (Peptidase_S8)	Subtilase family	Subtilases are a family of serine proteases. They appear to have independently and convergently evolved an Asp/Ser/His catalytic triad, like that found in the trypsin serine proteases (see Pfam:PF00089). Structure is an alpha/beta fold containing a 7 ...	Subtilases are a family of serine proteases. They appear to have independently and convergently evolved an Asp/Ser/His catalytic triad, like that found in the trypsin serine proteases (see Pfam:PF00089). Structure is an alpha/beta fold containing a 7-stranded parallel beta sheet, order 2314567.	Domain
A	PF17766	Fibronectin type-III domain (fn3_6)	Fibronectin type-III domain	This FN3 like domain is found at the C-terminus of cucumisin proteins [1].	Domain
B	PF05922	Peptidase inhibitor I9 (Inhibitor_I9)	Peptidase inhibitor I9	This family includes the proteinase B inhibitor from Saccharomyces cerevisiae and the activation peptides from peptidases of the subtilisin family. The subtilisin propeptides are known to function as molecular chaperones, assisting in the folding of ...	This family includes the proteinase B inhibitor from Saccharomyces cerevisiae and the activation peptides from peptidases of the subtilisin family. The subtilisin propeptides are known to function as molecular chaperones, assisting in the folding of the mature peptidase [1], but have also been shown to act as 'temporary inhibitors' [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Cucumisin	peptidase activity catalytic activity, acting on a protein serine hydrolase activity serine-type peptidase activity hydrolase activity serine-type endopeptidase activity catalytic activity endopeptidase activity	organic substance metabolic process organonitrogen compound metabolic process proteolysis metabolic process primary metabolic process protein metabolic process macromolecule metabolic process	extracellular region cellular anatomical entity
B	Cucumisin	peptidase activity catalytic activity, acting on a protein serine hydrolase activity serine-type peptidase activity hydrolase activity serine-type endopeptidase activity catalytic activity endopeptidase activity	organic substance metabolic process organonitrogen compound metabolic process proteolysis metabolic process primary metabolic process protein metabolic process macromolecule metabolic process	extracellular region cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR015500	Peptidase S8, subtilisin-related	Family
A	IPR023828	Peptidase S8, subtilisin, Ser-active site	Active Site
A	IPR036852	Peptidase S8/S53 domain superfamily	Homologous Superfamily
A	IPR041469	Subtilisin-like protease, fibronectin type-III domain	Domain
A	IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	Domain
A	IPR045051	Subtilisin-like protease	Family
A	IPR034197	Cucumisin-like catalytic domain	Domain
A	IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	Homologous Superfamily
A	IPR000209	Peptidase S8/S53 domain	Domain
B	IPR015500	Peptidase S8, subtilisin-related	Family
B	IPR023828	Peptidase S8, subtilisin, Ser-active site	Active Site
B	IPR036852	Peptidase S8/S53 domain superfamily	Homologous Superfamily
B	IPR041469	Subtilisin-like protease, fibronectin type-III domain	Domain
B	IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	Domain
B	IPR045051	Subtilisin-like protease	Family
B	IPR034197	Cucumisin-like catalytic domain	Domain
B	IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	Homologous Superfamily
B	IPR000209	Peptidase S8/S53 domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.