4WQM

Structure of the toluene 4-monooxygenase NADH oxidoreductase T4moF, K270S K271S variant

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00970	e4wqmA2	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PF00970	ECOD (1.6)
A	PF00111	e4wqmA3	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: PF00111	ECOD (1.6)
A	PF00175	e4wqmA1	A: a/b three-layered sandwiches	X: Ferredoxin reductase-like, C-terminal NADP-linked domain (From Topology)	H: Ferredoxin reductase-like, C-terminal NADP-linked domain (From Topology)	T: Ferredoxin reductase-like, C-terminal NADP-linked domain	F: PF00175	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.10.20.30	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Beta-grasp domain	CATH (4.3.0)
A	2.40.30.10	Mainly Beta	Beta Barrel	Elongation Factor Tu (Ef-tu)	domain 3	CATH (4.3.0)
A	3.40.50.80	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00175	Oxidoreductase NAD-binding domain (NAD_binding_1)	Oxidoreductase NAD-binding domain	Xanthine dehydrogenases, that also bind FAD/NAD, have essentially no similarity.	Domain
A	PF00111	2Fe-2S iron-sulfur cluster binding domain (Fer2)	2Fe-2S iron-sulfur cluster binding domain	-	Domain
A	PF00970	Oxidoreductase FAD-binding domain (FAD_binding_6)	Oxidoreductase FAD-binding domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Toluene-4-monooxygenase electron transfer component	iron-sulfur cluster binding 2 iron, 2 sulfur cluster binding binding metal cluster binding small molecule binding ferredoxin-NAD(P) reductase activity oxidoreductase activity oxidoreductase activity, acting on iron-sulfur proteins as donors oxidoreductase activity, acting on iron-sulfur proteins as donors, NAD or NADP as acceptor ferredoxin-NAD+ reductase activity catalytic activity cation binding ion binding metal ion binding	cellular response to stimulus catabolic process response to stimulus hydrocarbon catabolic process hydrocarbon metabolic process response to xenobiotic stimulus cellular catabolic process organic substance metabolic process toluene catabolic process toluene-containing compound catabolic process benzene-containing compound metabolic process cellular response to xenobiotic stimulus cellular metabolic process toluene-containing compound metabolic process cellular response to stimulus catabolic process response to stimulus hydrocarbon catabolic process hydrocarbon metabolic process response to xenobiotic stimulus cellular catabolic process organic substance metabolic process toluene catabolic process toluene-containing compound catabolic process benzene-containing compound metabolic process cellular response to xenobiotic stimulus cellular metabolic process toluene-containing compound metabolic process xenobiotic catabolic process organic cyclic compound catabolic process xenobiotic metabolic process organic cyclic compound metabolic process cellular response to chemical stimulus response to chemical metabolic process cellular process toluene metabolic process organic substance catabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR001709	Flavoprotein pyridine nucleotide cytochrome reductase	Domain
A	IPR001433	Oxidoreductase FAD/NAD(P)-binding	Domain
A	IPR036010	2Fe-2S ferredoxin-like superfamily	Homologous Superfamily
A	IPR001041	2Fe-2S ferredoxin-type iron-sulfur binding domain	Domain
A	IPR006058	2Fe-2S ferredoxin, iron-sulphur binding site	Binding Site
A	IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	Homologous Superfamily
A	IPR008333	Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain	Domain
A	IPR017938	Riboflavin synthase-like beta-barrel	Homologous Superfamily
A	IPR017927	FAD-binding domain, ferredoxin reductase-type	Domain
A	IPR012675	Beta-grasp domain superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.